We narrowed to 2,111 results for: D10

1. pST1374-D10A-GCN4

   Plasmid

   #113025

   Purpose

   Expresses D10A-GCN4 in mammalian cells

   Depositor

   Xingxu Huang

   Article

   Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

   Insert

   D10A-GCN4 (GCN4 S. pyogenes, Budding Yeast)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CMV

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX335A_hCas9(D10A)_IRF8gRNA2

   Plasmid

   #89720

   Purpose

   Knockout IRF8 in human cells

   Depositor

   Martin Zenke

   Article

   Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

   Insert

   IRF8 gRNA2 (IRF8 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE-PIK3CB-D1067Y

   Plasmid

   #116551

   Purpose

   Lentiviral expression of PIK3CB D1067Y

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   PIK3CB (PIK3CB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D1067Y

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUNIV-SIG-5HTR4D100A

   Plasmid

   #16312

   Depositor

   Bruce Conklin

   Article

   Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

   Insert

   h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (HTR4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Added FLAG tag; Aspartic Acid 100 changed to Alan…

   Promoter

   Available since

   Jan. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pRK5-mFzd10-1D4

   Plasmid

   #42272

   Depositor

   Jeremy Nathans, Chris Garcia

   Article

   Yu et al Development. 2012 Dec 1;139(23):4383-94.

   Insert

   Frizzled 10 (Fzd10 Mouse)
   

   Use

   Tags

   1D4

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pMIR-D10 UTR

   Plasmid

   #19117

   Depositor

   Bob Weinberg

   Article

   Ma et al Nature. 2007 Oct 11. 449(7163):682-8.

   Insert

   HOXD10 3' UTR (HOXD10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pMIR-D10 mutUTR

   Plasmid

   #19116

   Depositor

   Bob Weinberg

   Article

   Ma et al Nature. 2007 Oct 11. 449(7163):682-8.

   Insert

   HOXD10 mut 3' UTR (HOXD10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

8. G273D HA-dynamin 1 pUHD10-3

   Plasmid

   #34689

   Depositor

   Sandra Schmid

   Article

   Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

   Insert

   dynamin 1 (DNM1 Human)
   

   Use

   Tags

   HA (variant)

   Expression

   Mammalian

   Mutation

   G273D

   Promoter

   Available since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pBS cHoxD10 (CT#718)

   Plasmid

   #14010

   Depositor

   Cliff Tabin

   Insert

   HoxD10
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 2xHA MED10 pBacPAK8

    Plasmid

    #17321

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Koutelou et al J Biol Chem. 2007 Dec 12. ():.

    Insert

    MED10 (Med10 Mouse)
    

    Use

    Tags

    2xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 11, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pLV-EF1a-V5-Mfsd10

    Plasmid

    #120238

    Purpose

    Expresses V5-tagged Mfsd10 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Mfsd10 (Mfsd10 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FZR1 D10.1 gRNA

    Plasmid

    #90693

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FZR1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    FZR1 (Guide Designation D10.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJExpress401-KDAC8 D101N

    Plasmid

    #224348

    Purpose

    Expresses human KDAC8 (HDAC8) D101N in E. coli

    Depositor

    Terry Watt

    Article

    Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

    Insert

    KDAC8 (HDAC8 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    Aspartate 101 mutated to asparagine

    Promoter

    T5

    Available since

    Feb. 19, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pGEX-parkin D106A

    Plasmid

    #227362

    Purpose

    For bacterial expression of parkin with a mutated ACT.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin D106A (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJExpress401-KDAC8 D101R

    Plasmid

    #224349

    Purpose

    Expresses human KDAC8 (HDAC8) D101R in E. coli

    Depositor

    Terry Watt

    Article

    Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

    Insert

    KDAC8 (HDAC8 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    Aspartate 101 mutated to arginine

    Promoter

    T5

    Available since

    Oct. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pJExpress401-KDAC8 D101A

    Plasmid

    #224347

    Purpose

    Expresses human KDAC8 (HDAC8) D101A in E. coli

    Depositor

    Terry Watt

    Article

    Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

    Insert

    KDAC8 (HDAC8 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    Aspartate 101 mutated to alanine

    Promoter

    T5

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. Salsa-BC238-P3D10

    Plasmid

    #203079

    Purpose

    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC238

    Depositor

    Noah Palm

    Article

    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

    Insert

    EGFP/BC238
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet Response Element (TRE)

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Salsa-BC142-P2D10

    Plasmid

    #202983

    Purpose

    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC142

    Depositor

    Noah Palm

    Article

    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

    Insert

    EGFP/BC142
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet Response Element (TRE)

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mito-PDCD10-mScarlet-I

    Plasmid

    #208887

    Purpose

    Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM3 (PDCD10 Human)
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    PDCD10 targeted to the mitonchondria

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Salsa-BC46-P1D10

    Plasmid

    #202887

    Purpose

    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC46

    Depositor

    Noah Palm

    Article

    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

    Insert

    EGFP/BC46
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet Response Element (TRE)

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

    Plasmid

    #199253

    Purpose

    Expression construct encoding the high specificity SpCas9-KA-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    High specificity S. pyogenes Cas9-D10A-KA nickase
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A K848A

    Promoter

    CAG promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV_SEPT9_i1 D10-25-msfGFP

    Plasmid

    #180334

    Purpose

    mammalian expression of human SEPT9_i1 D10-25 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 D10-25

    Promoter

    CMV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-Ace1_Q89D_D100N_E206V-HaloTag

    Plasmid

    #129255

    Purpose

    Express Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace1_Q89D_D100N_E206V-HaloTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q89D, D100N, E206V

    Promoter

    CAG

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG-Ace1_Q89D_D100N_E206V-HaloTag-ST

    Plasmid

    #129260

    Purpose

    Express soma-localized Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace1_Q89D_D100N_E206V-HaloTag-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q89D, D100N, E206V

    Promoter

    CAG

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. phSOD1-D101G-N’His

    Plasmid

    #117704

    Purpose

    mutation on stability/solubility of SOD1

    Depositor

    Elizabeth Fisher

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    His tag with Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    D101G

    Promoter

    T7

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. KIF4A D10.2 gRNA

    Plasmid

    #90725

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KIF4A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    KIF4A (Guide Designation D10.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NUDC D10.4 gRNA

    Plasmid

    #90807

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUDC

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NUDC (Guide Designation D10.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LSM4 D10.3 gRNA

    Plasmid

    #90737

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LSM4

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    LSM4 (Guide Designation D10.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX335A_hCas9(D10A)_IRF8gRNA4

    Plasmid

    #89722

    Purpose

    Knockout IRF8 in human cells

    Depositor

    Martin Zenke

    Article

    Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

    Insert

    IRF8 gRNA4 (IRF8 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX335A_hCas9(D10A)_IRF8gRNA3

    Plasmid

    #89721

    Purpose

    Knockout IRF8 in human cells

    Depositor

    Martin Zenke

    Article

    Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

    Insert

    IRF8 gRNA3 (IRF8 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. hRad51(A89E)–Cas9(D10A)

    Plasmid

    #125563

    Purpose

    Expresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(A89E)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. LD101: pMVP (L3-L2) eCFP + WPRE

    Plasmid

    #121775

    Purpose

    pMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eCFP + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-CMV-SpCas9D10A nickase

    Plasmid

    #216737

    Purpose

    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMVd1

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pKMD106e - intein-Cas9(S219)

    Plasmid

    #64190

    Purpose

    Expresses intein-Cas9(S219) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

    Plasmid

    #98974

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 3 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRS315e_pGal-nCas9(D10A)-PmCDA1

    Plasmid

    #79617

    Purpose

    Expresses nCas9(D10A)-PmCDA1 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Yeast

    Mutation

    D10A for nickase Cas9

    Promoter

    pGal1

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. hRad51(K133R)–Cas9(D10A)

    Plasmid

    #125568

    Purpose

    Expresses the Rad51(K133R)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(K133R)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pX551-miniCMV-SpCas9D10A nickase

    Plasmid

    #216735

    Purpose

    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    T7 and mini-CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid

    #98975

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 4 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

    Plasmid

    #98972

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 1 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

    Plasmid

    #98973

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 2 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pKMD106h - intein-Cas9(C574)

    Plasmid

    #64191

    Purpose

    Expresses intein-Cas9(C574) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pPD1019 TRE3GV DsRedExpress2 in TU2

    Plasmid

    #139254

    Purpose

    TRE3GV promoter and DsRedExpress2 in TUPV2

    Depositor

    Joshua Leonard

    Insert

    DsRedExpress2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GV

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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44. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid

    #60600

    Purpose

    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Inserts

    CRISPR Cas9 D10A
    puromycin resistance gene
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Changed Asp 10 to Ala, Codon usage optimized for …

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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45. pX330-Flag-eSpCas9-D10A

    Plasmid

    #80449

    Purpose

    enhanced SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    ehSpCas9_D10A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCI-FLAG-mTUT4(D1028A)

    Plasmid

    #60220

    Purpose

    Mammalian expression of FLAG-tagged catalytic mutant of TUT4

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

    Insert

    TUT4 (Tut4 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D1028A

    Promoter

    CMV

    Available since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

47. pGEX6P1‐Afu‐rnhB D101N

    Plasmid

    #108695

    Purpose

    For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    rnhB
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    D101N catalytic site mutation

    Promoter

    tac

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. pPD1025 EF1alpha TetON3G in TU6

    Plasmid

    #139262

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV6

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pPD1026 EF1alpha TetON3G in TU2

    Plasmid

    #139258

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV2

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pPD107 VP16-ZF43(RARR)

    Plasmid

    #138812

    Purpose

    Constitutive expression of VP16-ZF43(RARR) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF43(RARR)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R15A

    Promoter

    CMV

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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