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We narrowed to 195 results for: RBM

Showing: 101 - 150 of 195 results

  1. pcDNA3.1-3'3xFlag

    Plasmid
    #208616
    Purpose
    Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208616-plasmid-map-sequence-id-418312

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  2. pcDNA3.1-StrepII

    Plasmid
    #208617
    Purpose
    Empty vector for mammalian expression, insert a Strep II tag (with ATG) using XbaI and XhoI site of pcDNA3.1 (-), both site retained
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Strep II
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208617-plasmid-map-sequence-id-418313

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  3. pET28-H12TEV

    Plasmid
    #208635
    Purpose
    for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208635-plasmid-map-sequence-id-433640

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  4. pET28-3'TwStrep

    Plasmid
    #208053
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208053-plasmid-map-sequence-id-415923

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  5. pET28-5'TwStrep

    Plasmid
    #208620
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208620-plasmid-map-sequence-id-415919

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  6. pcDNA3.1-3'TwStrep

    Plasmid
    #208618
    Purpose
    Empty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Twin Strep
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208618-plasmid-map-sequence-id-418314

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  7. pUC-ODN

    Plasmid
    #208642
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Long single strand dna production
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208642-plasmid-map-sequence-id-418350

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  8. pcDNA3.1-Flag

    Plasmid
    #208051
    Purpose
    Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208051-plasmid-map-sequence-id-418310

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  9. pET28-RPL3-Twstrep

    Plasmid
    #208623
    Purpose
    for E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) (RPL3 Human)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208623-plasmid-map-sequence-id-418316

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  10. pET28-RPL3L-TwStrep

    Plasmid
    #208649
    Purpose
    for E. coli expression of C-ter Twin Strep tagged human RPL3L (ENST00000268661), contain SNP rs753338989, rs746044959, rs752134949
    Depositor
    Quanfu Ma
    Insert
    human RPL3L (ENST00000268661) (RPL3L Human)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    contain rs753338989, rs746044959, rs752134949, pr…
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208649-plasmid-map-sequence-id-433652

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  11. pcDNA3.1-CRISPR-Puro

    Plasmid
    #208646
    Purpose
    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208646-plasmid-map-sequence-id-418362

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  12. pUC-T7-J1~398-Luc

    Plasmid
    #208639
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19
    Depositor
    Quanfu Ma
    Insert
    Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
    Use
    Luciferase; In vitro transcription template
    Tags
    Firefly Luciferase (F-Luc and T7 promoter
    Expression
    Mutation
    nt 1~398, in frame with donstream F-Luc
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208639-plasmid-map-sequence-id-418341

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  13. pET28-eIF4E-TwStrep

    Plasmid
    #208638
    Purpose
    for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)
    Depositor
    Quanfu Ma
    Insert
    human eIF4E (ENST00000450253) (EIF4E Human)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208638-plasmid-map-sequence-id-415918

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  14. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

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  16. pET28-RPL3-1~41-mEGFP(A206K)-TwStrep

    Plasmid
    #208628
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 1~41, other residues not included
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208628-plasmid-map-sequence-id-433639

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  17. pET28-RPL3-Δ1~34,Δ215~284-TwStrep

    Plasmid
    #208630
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    Δ1~34,Δ215~284
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208630-plasmid-map-sequence-id-418338

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  18. pET28-RPL3-53~77-mEGFP(A206K)-TwStrep

    Plasmid
    #208632
    Purpose
    for E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 53~77, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208632-plasmid-map-sequence-id-418343

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  19. pET28-RPL3-1~77-mEGFP(A206K)-TwStrep

    Plasmid
    #208624
    Purpose
    for E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 1~77, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208624-plasmid-map-sequence-id-418336

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  21. pET28-H12TEV-hEIF5B-1~1220(FL)

    Plasmid
    #208636
    Purpose
    for E. coli expression of human eIF5B (ENST00000289371)
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) (EIF5B Human)
    Use
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    Contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    208636-plasmid-map-sequence-id-433645

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  22. pET28-RPL3-72~197-mEGFP(A206K)-TwStrep

    Plasmid
    #208625
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa72~197, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 72~197 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 72~197, other residues not included
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208625-plasmid-map-sequence-id-433638

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  23. pUC-ODN-hsRPL3-CterTwStrep

    Plasmid
    #208644
    Purpose
    for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.
    Depositor
    Quanfu Ma
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
    Use
    CRISPR; Ssdna donnor preparation
    Tags
    Twin Strep
    Expression
    Mutation
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208644-plasmid-map-sequence-id-418358

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  24. pcDNA3.1-msEGFP1.8

    Plasmid
    #208619
    Purpose
    Mammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.
    Depositor
    Quanfu Ma
    Insert
    msEGFP1.8
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (compared with pEGFP-C1, protein level) S30R, Y39…
    Promoter
    CMV
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208619-plasmid-map-sequence-id-418315

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  25. pET28-H12TEV-hEIF5B-587~1220

    Plasmid
    #208637
    Purpose
    for E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
    Use
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208637-plasmid-map-sequence-id-433646

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  26. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

    Plasmid
    #208626
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 191~307, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208626-plasmid-map-sequence-id-415912

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  27. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid
    #208627
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 299~398, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208627-plasmid-map-sequence-id-415915

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  28. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

    Plasmid
    #208629
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 212~285, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208629-plasmid-map-sequence-id-415924

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  29. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid
    #208647
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.
    Depositor
    Quanfu Ma
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Synthetic, Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208647-plasmid-map-sequence-id-418374

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  30. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid
    #208979
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.
    Depositor
    Quanfu Ma
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Synthetic, Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208979-plasmid-map-sequence-id-418375

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  33. SMN2E7-Fluc-Nluc-reporter

    Plasmid
    #221005
    Purpose
    Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    Fluc-SMN2E7-STOP-Nluc
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    221005-plasmid-map-sequence-id-437012

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  36. pUC-T7-hTP53-P0~670-FLuc

    Plasmid
    #208640
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.
    Depositor
    Quanfu Ma
    Insert
    TP53, UTR and partial CDS (TP53 Human)
    Use
    Luciferase; In vitor transcription template
    Tags
    Firefly Luciferase (F-Luc) and T7 promoter
    Expression
    Mutation
    UTR and partial CDS in-frame with F-Luc
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208640-plasmid-map-sequence-id-418347

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  37. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

    Plasmid
    #208645
    Purpose
    Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.
    Depositor
    Quanfu Ma
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
    Use
    CRISPR; Ssdna donnor preparation for knock-in of…
    Tags
    Twin Strep
    Expression
    Mutation
    Two SNP (rs749555789, rs760194863) were introduce…
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208645-plasmid-map-sequence-id-415926

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  38. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid
    #221004
    Purpose
    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    CasRx 30nt processed direct repeat with SD1 mutation
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    DR1 A>T
    Promoter
    U6
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221004-plasmid-map-sequence-id-436033

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  48. TFORF1311

    Plasmid
    #143786
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RBMS1 (RBMS1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143786-plasmid-map-sequence-id-396236

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  49. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

    Plasmid
    #229498
    Purpose
    This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.
    Depositor
    Davide Ruggero
    Article
    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/
    Insert
    MYC 5'Untranslated region + destabilized GFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229498-plasmid-map-sequence-id-456853

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Showing: 101 - 150 of 195 results