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Plasmid#222915PurposeHomology directed repair template for knocking in mGreenLantern reporter to TFAP2C.DepositorInsertmGreenLantern
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHDR-PMG (pXK-1650)
Plasmid#208858PurposeHDR-base selectable traffic light reporter (TLR) for helping to screen HDR-editing positive cells. Key Construct: iPuroR*-CMV-PuroR.L-mCherry-PuroR.R-GFP.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPW3755 : HDR-HsIRE1a-10xGS-HaloTag
Plasmid#185677PurposeHDR template used for endogenous CRISPR editing of ERN1 gene, inserting a C-terminal HaloTag.DepositorInsertERN1 homology arms (ERN1 Synthetic, Human)
UseCRISPRTagsHaloTagExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHDR-FOXL2-T2A-tdTomato-PuroTK
Plasmid#192892PurposeHDR donor for inserting FOXL2-T2A-tdTomato reporterDepositorInsertFOXL2-tdTomato (FOXL2 Human)
UseCRISPRTagsExpressionMutationT2A-tdTomato fluorescent reporterPromoterPGKAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFlpStop-HDR-UAS-2.1-tdTom
Plasmid#89148PurposeFlpStop plasmid for CRISPR/HDR. Has MCS for homology armsDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoter5XUAS, 3XP3-dsRedAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
3xFlag-HaloTag-SUZ12 HDR - pDY082
Plasmid#171110PurposeHomology directed repair plasmid for generating HaloTag-SUZ12 cellsDepositorInsertSUZ12 (SUZ12 Human)
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHDR-CDKN2A-Ex2-CMV-EGFP
Plasmid#110734PurposeRecombination template for replacing CDKN2A Exon2 with CMV-EGFPDepositorInsertEGFP
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro
Plasmid#196196Purposehomologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locusDepositorInsertG3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
UseHomology repair donorTagsV5-APEX2-mScarlet-IExpressionMutationPromoterAvailable sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xFlag-HaloTag-EZH2 HDR - pDY053
Plasmid#171108PurposeHomology directed repair plasmid for generating HaloTag-EZH2 cellsDepositorInsertEZH2 (EZH2 Human)
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
ppk301-T2A-QF2 HDR plasmid
Plasmid#130667PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 geneDepositorInsertppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
UseDonor templateTagsExpressionMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gr4-T2A-QF2 HDR plasmid
Plasmid#140944PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Gr4 geneDepositorInsertGr4-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr4-right-HDR-arm
UseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHD-FlpTag-DsRed-HDR-ph2
Plasmid#231902PurposedsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 2.DepositorInsertFrt-Frt14-SD-GFP-SA-Frt-Frt14
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHD-FlpTag-DsRed-HDR-ph1
Plasmid#231901PurposedsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 1.DepositorInsertFrt-Frt14-SD-GFP-SA-Frt-Frt14
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHD-FlpTag-DsRed-HDR-ph0
Plasmid#231900PurposedsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 0.DepositorInsertFrt-Frt14-SD-GFP-SA-Frt-Frt14
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
fru-T2A-QF2 HDR plasmid
Plasmid#141099PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru geneDepositorInsertfru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
UseDonor templateTagsExpressionMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY153: Flag(SUZ12) WT HDR plasmid
Plasmid#170793PurposeWT homology directed repair plasmid for performing genome-editing on the SUZ12 LocusDepositorInsertSUZ12 (SUZ12 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ir7f-T2A-QF2 HDR plasmid
Plasmid#140942PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f geneDepositorInsertIr7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
UseTagsExpressionMutation4 point mutations (annotated on plasmid map) were…PromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ir7a-T2A-QF2 HDR plasmid
Plasmid#140943PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7a geneDepositorInsertIr7a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7a-right-HDR-arm
UseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-P2A-3xFLAG-hEEA1 HDR template
Plasmid#214989PurposeEndogenously tag human EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.DepositorInsertEEA1 (EEA1 Human)
UseCRISPRTags3xFLAGExpressionMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-P2A-3xFLAG-msEEA1 HDR template
Plasmid#214990PurposeEndogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.DepositorInsertEEA1 (Eea1 Mouse)
UseCRISPRTags3xFLAGExpressionMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only