We narrowed to 282 results for: PPIC

1. pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)

   Plasmid

   #227609

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 2-544, 651-757 only [TM deleted]

   Promoter

   AOX1

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPICZ A-SLC-His+MFN2-Strep (SB255)

   Plasmid

   #227604

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPICZ A-SLC25A46[R247A,R347A]-His (SB257)

   Plasmid

   #227606

   Purpose

   Inducible expression of His-tagged SLC25A46 with R247A and R347A mutations in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   SLC25A46 (SLC25A46 Human)
   

   Tags

   10xHis

   Expression

   Yeast

   Mutation

   R247A,R347A

   Promoter

   AOX1

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZ-pre-Ost1-pro-alphaf-Btl2

   Plasmid

   #117665

   Purpose

   Study secretion efficiency of Btl2

   Depositor

   Benjamin Glick, Pau Ferrer

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-Ost1-pro-alphaf-Btl2
   

   Expression

   Yeast

   Mutation

   This plasmid encodes the lipase Btl2 together wit…

   Promoter

   pAOX1

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson

   Plasmid

   #117663

   Purpose

   Test intracellular localisation of E2-Crimson and study the secretion efficiency

   Depositor

   Benjamin Glick, Pau Ferrer

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pOst1-pro-af(MUT2)-E2-Crimson
   

   Expression

   Yeast

   Mutation

   This plasmid encodes the fluorescent protein E2-C…

   Promoter

   pAOX1

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

   Plasmid

   #227611

   Purpose

   Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Tags

   MBP, 10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 2-88 only

   Promoter

   AOX1

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

   Plasmid

   #227610

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 2-400, 706-757 [TM and HB2 deleted]

   Promoter

   AOX1

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKdH_P-GAP-ScADH2

   Plasmid

   #126734

   Purpose

   yeast plasmid overexpressing ADH2 from Saccharomyces cerevisiae

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   adh2
   

   Tags

   6xHis

   Expression

   Yeast

   Promoter

   GAP

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKdH_P-GAP-ScACS1*

   Plasmid

   #126736

   Purpose

   yeast plasmid overexpressing ACS1 from Saccharomyces cerevisiae

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   acs1*
   

   Tags

   6xHis,Myc

   Expression

   Yeast

   Mutation

   T3542C and A30S, P113S, and S629N (please see dep…

   Promoter

   GAP

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKdH_P-GAP-ScADH2+P-GAP*-ScACS1*

    Plasmid

    #126738

    Purpose

    yeast plasmid overexpressing ADH2 and ACS1 from Saccharomyces cerevisiae

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    adh2,acs1*
    

    Tags

    6xHis,Myc

    Expression

    Yeast

    Mutation

    T3542C

    Promoter

    GAP

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKdH_P-GAP-ScADH2+P-GAP*-ScALD6

    Plasmid

    #126737

    Purpose

    yeast plasmid overexpressing ADH2 and ALD6 from Saccharomyces cerevisiae

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    adh2,ald6
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*

    Plasmid

    #126739

    Purpose

    yeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiae

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    adh2, acs1*, ald6
    

    Tags

    6xHis,Myc

    Expression

    Yeast

    Mutation

    T3542C and A30S, S629N (please see depositor comm…

    Promoter

    GAP

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEZYpci

    Plasmid

    #18707

    Purpose

    pPIC3.5K converted Gateway Destionation vector for multiple copy integration of gene of interest into Pichia

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSF4 CMV intron renilla TRICK CTE polyA

    Plasmid

    #84443

    Purpose

    TRICK reporter mRNA

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    Renill-TRICK
    

    Use

    Luciferase; Frt

    Expression

    Mammalian

    Promoter

    Tet-CMV

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. phage UBC NLS-HA-2XMCP-tagRFPt

    Plasmid

    #64541

    Purpose

    Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to tagRFP.

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    MCP
    

    Use

    Lentiviral

    Tags

    HA, NLS, and tagRFP-T

    Expression

    Mammalian

    Promoter

    human ubiquitin C promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. phage ubc nls ha pcp gfp

    Plasmid

    #64539

    Purpose

    Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    PCP
    

    Use

    Lentiviral

    Tags

    EGFP, FactorXa site, HA, and NLS

    Expression

    Mammalian

    Promoter

    human ubiquitin C promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHsp83-NLS-HA-2xMCP-2xTagRFP-T

    Plasmid

    #71242

    Purpose

    Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.

    Depositor

    Anne Ephrussi

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    NLS-2xMCP-2xTagRFP-T
    

    Use

    P element-mediated germline transformation

    Tags

    TagRFP-T

    Expression

    Insect

    Promoter

    Hsp83

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. phage ubc nls ha 2xmcp HALO

    Plasmid

    #64540

    Purpose

    Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    MCP
    

    Use

    Lentiviral

    Tags

    HA, HALO, and NLS

    Expression

    Mammalian

    Promoter

    human ubiquitin C promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHsp83-NLS-HA-2xPCP-2xGFP

    Plasmid

    #71243

    Purpose

    Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

    Depositor

    Anne Ephrussi

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    2xPCP-2xGFP
    

    Use

    P element-mediated germline transformation

    Tags

    EGFP

    Expression

    Insect

    Promoter

    Hsp83

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTMEM-PhoCl-Insert

    Plasmid

    #219674

    Purpose

    Caging at cytosolic N-ter of POI: TMEM-PhoCl-rtta3mScarlet

    Depositor

    Helge Ewers

    Article

    Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

    Insert

    TMEM-PhoCl-rtTA3
    

    Tags

    PhoCl

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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