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pCS2+-FLAG3-hsAgo2E-D

Plasmid

#221721

Purpose

Expression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2E-D (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

E637D in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-hsAgo2D-A

Plasmid

#221720

Purpose

Expression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2D-A (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

D669A in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-hsAgo2

Plasmid

#221719

Purpose

Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2 (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsAGO4_F

Plasmid

#146317

Purpose

Mammalian Expression of HsAGO4

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO4 (AGO4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-HsAGO2_E

Plasmid

#146224

Purpose

Insect Expression of HsAGO2

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2 (AGO2 Human)

Use

Tags

Expression

Insect

Mutation

Contains a K660E mutation compared to the sequenc…

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsAGO1_C

Plasmid

#146083

Purpose

Mammalian Expression of HsAGO1

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO1 (AGO1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsAGO4_C

Plasmid

#146085

Purpose

Mammalian Expression of HsAGO4

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO4 (AGO4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsAGO3_C

Plasmid

#146084

Purpose

Mammalian Expression of HsAGO3

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO3 (AGO3 Human)

Use

Tags

Expression

Mammalian

Mutation

T25A

Promoter

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH

Plasmid

#148855

Purpose

Mammalian Expression of HsAGO3-sgRNA

Depositor

Elisa Izaurralde

Insert

HsAGO3-sgRNA (AGO3 S.pyogenes)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CJ34 (miR-1003 3'ssAG->GA)

Plasmid

#17770

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1003 minigene 3'ssAG->GA

Use

Drosophila p-element vector

Tags

Expression

Mutation

splice site mutation

Promoter

Available since

May 12, 2008

Availability

Academic Institutions and Nonprofits only
pCS2+-FLAG3-drN-hsAgo2

Plasmid

#221724

Purpose

Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for zebrafish Ago2 N-domain.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drN-hsAgo2 (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

Swap of N-domain for zebrafish Ago2 N-domain

Promoter

simian CMV IE94

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsAGO2-F2V2_L

Plasmid

#146885

Purpose

Mammalian Expression of HsAGO2-F2V2

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsAGO2-F2V2 (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

sequence differs in the first 7 AA from the seque…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsAGO2-F2V2_F

Plasmid

#146287

Purpose

Mammalian Expression of HsAGO2-F2V2

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2-F2V2 (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

sequence differs in the first 7 AA from the seque…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsAGO2-F470V_F

Plasmid

#146288

Purpose

Mammalian Expression of HsAGO2-F470V

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2-F470V (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

sequence differs in the first 7 AA from the seque…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsAGO2-F505V_F

Plasmid

#146289

Purpose

Mammalian Expression of HsAGO2-F505V

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2-F505V (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

sequence differs in the first 7 AA from the seque…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-HsAGO2-F470V_E

Plasmid

#146225

Purpose

Insect Expression of HsAGO2-F470V

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2-F470V (AGO2 Human)

Use

Tags

Expression

Insect

Mutation

sequence differs in the first 7 AA from the seque…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-HsAGO2-F505V_E

Plasmid

#146226

Purpose

Insect Expression of HsAGO2-F505V

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2-F505V (AGO2 Human)

Use

Tags

Expression

Insect

Mutation

sequence differs in the first 7 AA from the seque…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsAGO2-K663AL697Y_AC

Plasmid

#148427

Purpose

Mammalian Expression of HsAGO2-K663AL697Y

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsAGO2-K663AL697Y (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutations and the sequence differs i…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsAGO2-P593GA623FK663AL697Y_AC

Plasmid

#148428

Purpose

Mammalian Expression of HsAGO2-P593GA623FK663AL697Y

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsAGO2-P593GA623FK663AL697Y (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutations and the sequence differs i…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-Puro-V2.0-sg1HsAGO2_AH

Plasmid

#148848

Purpose

Mammalian Expression of HsAGO2-sgRNA1

Depositor

Elisa Izaurralde

Insert

HsAGO2-sgRNA1 (AGO2 S.pyogenes)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge