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Plasmid#131299PurposeCAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).DepositorInsertNLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_P2Aq(1st)
Plasmid#190912Purpose2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assemblyDepositorInsertP2A
UseTagsExpressionMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-BREP
Plasmid#172337PurposeBRET-Based Bioluminescent Red ProteinDepositorInsertBREP
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEW8
Plasmid#84238PurposePlasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiaeDepositorInsertHA-mRuby-P2A-eGFP
UseTagsHA-mRuby and eGFPExpressionYeastMutationPromoterGPDAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-GFP-P2A
Plasmid#80807PurposeGFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptideDepositorInsertGFP-P2A
UseTagsExpressionMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-BREP
Plasmid#172340PurposeAAV transfer plasmid for BREP (bioluminescent red protein)DepositorInsertBREP
UseAAVTagsExpressionMutationPromoterhSyn promoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS1018-Tier1-PhCMV-p2A
Plasmid#169491PurposeTier-1 vector encoding a self-cleaving peptides p2a sequence (PhCMV-p2A-pA).DepositorInsertPCMV-driven 2A sequence of porcine teschovirus-1
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
murine TCR OTII-2A.pMIG II
Plasmid#52112Purposeretroviral expression of both TCRalpha and TCRbeta using a single vectorDepositorUseRetroviralTagsP2A (from porcine teschovirus-1)ExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_D2A_tdTomato
Plasmid#184045PurposeExpresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)DepositorInsertsEnhanced Green Fluorescent Protein
tdTomato
Dual 2A Peptide Sequence
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-PT2A
Plasmid#202138PurposeLevel 0 plasmid containing a dual 2A (P2A and T2A) peptide sequence with CD overhangs used to build level 1 constructsDepositorInsertDual 2A peptide
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGs-NLS-TIR1_P2A_NES-TIR1
Plasmid#117699PurposeExpression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNADepositorInsertsTRANSPORT INHIBITOR RESPONSE 1
TRANSPORT INHIBITOR RESPONSE 1
UseTagsHA tag, Myc-NES, and NLSExpressionMammalianMutationcodon optimized for murine expressionPromoterNone, 2A peptide from porcine teschovirus-1 polyp…Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only