We narrowed to 4,416 results for: Pet2
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Plasmid#114159PurposeExpresses ClbI S178ADepositorInsertclbI
Tags6xHis and 6xHis-Thrombin siteExpressionBacterialMutationMutated S178 to AlaAvailable SinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbH-C-A2-PCP
Plasmid#114157PurposeExpresses ClbH-C-A2-PCPDepositorInsertclbH
Tags6xHis-thrombin-T7 tagExpressionBacterialMutationOnly contains the C-A2-PCP domains of ClbHAvailable SinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P46A)
Plasmid#112721PurposeThis mutation (P46A) marks the first residue within the evolved region of U1A.DepositorInsert6His-TEV-TBP6.7(P46A)
Tags6His-TEVExpressionBacterialMutationP46APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47A)
Plasmid#112722PurposeArg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R47A)
Tags6His-TEVExpressionBacterialMutationR47APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47K)
Plasmid#112723PurposeArg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.DepositorInsert6His-TEV-TBP6.7(R47K)
Tags6His-TEVExpressionBacterialMutationR47KPromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48A)
Plasmid#112724PurposeGln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.DepositorInsert6His-TEV-TBP6.7(Q48A)
Tags6His-TEVExpressionBacterialMutationQ48APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48T)
Plasmid#112725PurposeThis mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.DepositorInsert6His-TEV-TBP6.7(Q48T)
Tags6His-TEVExpressionBacterialMutationQ48TPromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(T50A)
Plasmid#112727PurposeLav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.DepositorInsert6His-TEV-TBP6.7(T50A)
Tags6His-TEVExpressionBacterialMutationT50APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P51A)
Plasmid#112728PurposeSimilar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.DepositorInsert6His-TEV-TBP6.7(P51A)
Tags6His-TEVExpressionBacterialMutationP51APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R52A)
Plasmid#112729Purpose52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.DepositorInsert6His-TEV-TBP6.7(R52A)
Tags6His-TEVExpressionBacterialMutationR52APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q54A)
Plasmid#112730PurposeAlthough not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.DepositorInsert6His-TEV-TBP6.7(Q54A)
Tags6His-TEVExpressionBacterialMutationQ54APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RupA_T1-NHis
Plasmid#109259PurposeNRPS T domain with N-terminal 6His tagDepositorArticleInsertRupA_T1
TagsHis tagExpressionBacterialMutationcontains aa 917..1025 of WP_015523502.1PromoterT7Available SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-RupA_T2-R-CHis
Plasmid#109258PurposeNRPS di-domain containing TR domains with C-terminal 6His tagDepositorArticleInsertRupA_T2-R
TagsHis tag and S-TagExpressionBacterialMutationcontains aa 1939..2443 of WP_015523502.1PromoterT7Available SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RupA_R-NHis
Plasmid#109257PurposeNRPS R domain with N-terminal 6His tagDepositorArticleInsertRupA_R
TagsHis tagExpressionBacterialMutationcontains aa 2038..2443 of WP_015523502.1PromoterT7Available SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-RupA_C2-A2-T2-R-CHis
Plasmid#109256PurposeNRPS module containing CATR domains with C-terminal 6His tagDepositorArticleInsertRupA_C2-A2-T2-R
TagsHis tag and S-TagExpressionBacterialMutationcontains aa 1023..2443 of WP_015523502.1PromoterT7Available SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-RupA_C1-A1-T1-CHis
Plasmid#109255PurposeNRPS module containing CAT domains with C-terminal 6His tagDepositorArticleInsertRupA_C1-A1-T1
TagsHis tag and S-TagExpressionBacterialMutationcontains aa 1..1025 of WP_015523502.1PromoterT7Available SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Ec-ClpX D144L
Plasmid#111514PurposeExpresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144DepositorInsertClpX
TagsHis tagExpressionBacterialMutationAspartatic acid 144 to LeucinePromoterT7Available SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-1xTS
Plasmid#109418PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to one tyrosine sulfation (TS) motif. VHH-1xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys
Plasmid#110541PurposeBacterial protein of E.coli DHFR C85A/C152S mutantDepositorInsertecDHFR
ExpressionBacterialMutationC85 mutated to A85, C152 mutated to S152PromoterT7Available SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP
Plasmid#110828PurposeBacterial expression of E.coli DHFR N23PP mutantDepositorInsertecDHFR
ExpressionBacterialMutationN23 mutated to P23; a proline residue inserted be…PromoterT7Available SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b+R3(R112C/T180C)C
Plasmid#108194PurposeExpresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coliDepositorInsertPdR-PCNA3(R112C/T180C)-P450cam fusion protein
TagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b+3(R112C/T180C)C
Plasmid#108193PurposeExpresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coliDepositorInsertPCNA3(R112C/T180C)-P450cam fusion protein
TagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-Metavinculin tail
Plasmid#107152Purposebacterial expressionDepositorAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-EPN01(∆M)-His
Plasmid#84510PurposeE.coli version of EPN01(∆M)DepositorInsertEPN01(∆M)-His
TagsHis tagExpressionBacterialAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308/pET21a(+)
Plasmid#83293Purposecsd2, construct: 121-308, pET21a(+) no-tagDepositorInserthp1544
ExpressionBacterialMutationdeleted amino acid 1-120Available SinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd5_41-192/pET28b(+)
Plasmid#83296Purposecsd5, construct: 41-192, pET28b(+) N-tagDepositorInserthp1250
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-40Available SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd4_22-438/pET28b(+)
Plasmid#83295Purposecsd4, construct: 22-438, pET28b(+) N-tagDepositorInserthp1075
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-21Available SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd2_140-251/pET21a(+)
Plasmid#83294Purposecsd2, construct:140-251, pET21a(+) C-tagDepositorInserthp1544
TagsHis tagExpressionBacterialMutationdeleted amino acids 1-139 and 252-308Available SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd1_54-312/pET21a(+)
Plasmid#83291Purposecsd1, construct: 54-312, pET21a(+) C-tagDepositorInserthp1543
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-53Available SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308/pET28b(+)
Plasmid#83292Purposecsd2, construct: 121-308, pET28b(+) NC-tagDepositorInserthp1544
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-120Available SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd1_125-312/pET28b(+)
Plasmid#83290Purposecsd1, construct: 125-312, pET28b(+) NC-tagDepositorInserthp1543
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-124Available SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmUBB(SnaBI)
Plasmid#69558PurposeIn vitro transcription/translation (T7)DepositorInsertUBB (Ubb Mouse)
ExpressionBacterialMutationThe two last codons (TATTAA; encoding the last ty…PromoterT7Available SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmUBB(Y305P)
Plasmid#69557PurposeBacterial expression; in vitro transcription/translation (T7)DepositorInsertUBB (Ubb Mouse)
ExpressionBacterialMutationCodon TAT encoding tyrosine 305 was changed to CC…PromoterT7Available SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Cow 1-82)
Plasmid#62062PurposeExpression of Cow p53 (residues 1-82) in e. coliDepositorInsertp53
TagsHisExpressionBacterialMutationcontains TAD residues 1-82PromoterT7Available SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-73) (K24N)
Plasmid#62079PurposeExpression of human p53 (1-73) (K24N) in e. coliDepositorInsertp53 (TP53 Human)
TagsHisExpressionBacterialMutationcontains TAD residues 1-73; K24NPromoterT7Available SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Rabbit 1-87)
Plasmid#62058PurposeExpression of Rabbit p53 (residues 1-87) in e. coliDepositorInsertp53
TagsHisExpressionBacterialMutationcontains TAD residues 1-87PromoterT7Available SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (PPP)
Plasmid#62067PurposeExpression of human p53 (1-73) (P12A, P13A, P27A) in e. coliDepositorInsertp53 (TP53 Human)
TagsHisExpressionBacterialMutationContains TAD residues 1-73; P12A, P13A, P27APromoterT7Available SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab2(1-214)
Plasmid#59550Purposemakes 6x Fusion of Full length ceRab2DepositorInsert1-214
Available SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab5(1-208)
Plasmid#59551Purposemakes 6x Fusion of Full length ceRab5DepositorInsert1-208
Tags6x HisAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab10(1-201)
Plasmid#59552Purposemakes 6x Fusion of Full length ceRab10DepositorInsert1-208
Tags6x HisAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab11(1-211)
Plasmid#59553Purposemakes 6x Fusion of Full length ceRab11DepositorInsert1-211
Tags6x HisAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab21(1-207)
Plasmid#59554Purposemakes 6x Fusion of Full length ceRab21DepositorInsert1-207
Tags6x HisAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab21(1-209)
Plasmid#59555Purposemakes 6x Fusion of Full length ceRab35DepositorInsert1-209
Tags6x HisAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET29b-ClbP-S95A
Plasmid#48245PurposeUntagged, full-length protein with active site mutation Serine95 to Alanine for in vivo peptidase assaysDepositorInsertClbP
ExpressionBacterialMutationChanged Serine 95 to AlaninePromoterT7Available SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Ile170Thr
Plasmid#50725Purposeoverexpression of the human TPI1 Ile170Thr allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
Tags6xHIS tagExpressionBacterialMutationchanged Isoleucine 170 to ThreoninePromoterT7Available SinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-HpDsbG C162A
Plasmid#39330DepositorInsertoxidoreductase
TagsHis-tagExpressionBacterialMutationchanged Cysteine 162 to AlaninePromoterT7Available SinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)-HP0377 C92A
Plasmid#39335DepositorInsertoxidoreductase
TagsHis-tagExpressionBacterialMutationchanged Cysteine 92 to AlaninePromoterT7Available SinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pET28b-ZnF-UBPdel
Plasmid#90299Purposeprotein expressionDepositorInsertSAD1
TagsHis tagExpressionBacterialMutationZnF-UBP deletedAvailabilityAcademic Institutions and Nonprofits only -
pET28b-sad1-H79A
Plasmid#90303Purposeprotein expressionDepositorInsertSAD1
TagsHis tagExpressionBacterialMutationH79AAvailabilityAcademic Institutions and Nonprofits only