Addgene

Showing: 581 - 600 of 1036 results

Results per page:

Sort results by:

pCDNA3.1 hSCN5AdeltaSIV GFP Nter

Plasmid

#145770

Purpose

expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

Depositor

Hugues Abriel

Article

Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-GFP-AMA1-Flag(K546M/I547P/I548Y/I549Y)

Plasmid

#155308

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged AMA1 K546M/I547P/I548Y/I549Y variant

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfAMA1

Use

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Mutation

K546M/I547P/I548Y/I549Y

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pL-CRISPR.EFS.GFP

Plasmid

#57818

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

EFS and hU6

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
CL20_mEGFP_RUNX1_RUNX1T1

Plasmid

#205915

Purpose

Express mEGFP-tagged fusion protein, RUNX1_RUNX1T1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

RUNX1_RUNX1T1 (RUNX1, RUNX1T1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-GFP-EBA175-Flag(E1418K/P1424Q/Y1426S)

Plasmid

#155006

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfEBA175

Use

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Mutation

E1418K/P1424Q/Y1426S; insert codes for EBA175 res…

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1426H)

Plasmid

#155312

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1426H variant (residues 1284-1462) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfEBA175

Use

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Mutation

M1423R/P1424V/Y1426H; insert codes for EBA175 res…

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-GFP-EBA175-Flag(A1419R/P1424Q/Y1426S)

Plasmid

#155007

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfEBA175

Use

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Mutation

A1419R/P1424Q/Y1426S; insert codes for EBA175 res…

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-GFP-EBA175-Flag(S1421R/P1424Q/Y1426S)

Plasmid

#155008

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfEBA175

Use

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Mutation

S1421R/P1424Q/Y1426S; insert codes for EBA175 res…

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-GFP-EBA175-Flag(P1424I/Y1425I/Y1426I)

Plasmid

#154977

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424A/Y1425I/Y1426I variant (residues 1284-1462) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfEBA175

Use

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Mutation

P1424/Y1425I/Y1426I; insert codes for EBA175 resi…

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-GFP-IRES-hSNCA (A30P)

Plasmid

#185720

Purpose

AAV expression of GFP and human α-Synuclein with A30P mutation from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (SNCA Human)

Use

AAV

Tags

Expression

Mutation

Changed Ala 30 to Pro

Promoter

hSyn

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-GFP-IRES-hSNCA (S129D)

Plasmid

#185719

Purpose

AAV expression of GFP and human α-Synuclein with S129D mutation from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (SNCA Human)

Use

AAV

Tags

Expression

Mutation

Changed Ser 129 to Asp

Promoter

hSyn

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-GFP-IRES-hSNCA (S129A)

Plasmid

#185718

Purpose

AAV expression of GFP and human α-Synuclein with S129A mutation from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (SNCA Human)

Use

AAV

Tags

Expression

Mutation

Changed Ser 129 to Ala

Promoter

hSyn

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CL20_mEGFP_ETV6_RUNX1

Plasmid

#205818

Purpose

Express mEGFP-tagged fusion protein, ETV6_RUNX1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ETV6_RUNX1 (ETV6, RUNX1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CL20_mEGFP_RUNX1_CBFA2T3

Plasmid

#205914

Purpose

Express mEGFP-tagged fusion protein, RUNX1_CBFA2T3 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

RUNX1_CBFA2T3 (RUNX1, CBFA2T3 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(-6kb/-58)-GFP

Plasmid

#30460

Depositor

Gerhart Ryffel

Article

Ryffel et al Mech Dev. 2000 Jan . 90(1):65-75.

Insert

HNF1a promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP Rab11a S25N pcDNA3.1 Zeo (+)

Plasmid

#206080

Purpose

dominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recycling

Depositor

Annette Dolphin

Article

Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

Insert

RAB11A (RAB11A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S25N

Promoter

CMV

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Aurora A

Plasmid

#198402

Purpose

Expression of EGFP-tagged Aurora A in mammalian cells

Depositor

Kenji Sugimoto

Article

Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

Insert

Aurora A

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-C1-CAMKIIbeta

Plasmid

#21227

Depositor

Tobias Meyer

Article

Shen et al Neuron. 1998 Sep . 21(3):593-606.

Insert

CAMKIIbeta (Camk2b Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Lab plasmid KS 0002.

Promoter

Available since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Hyg-mEGFP-pp4640

Plasmid

#191846

Purpose

Expresses Salmon Alphavirus polyprotein with mEGFP N-terminal fusion

Depositor

Bertrand Collet

Article

Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

Insert

mEGFP-pp4640

Use

Tags

mEGFP

Expression

Mammalian

Mutation

mEGFP fusion in Nterminal, some silent point muta…

Promoter

CMV

Available since

Nov. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AICSDP-4:TUBA1B-mEGFP

Plasmid

#87421

Purpose

Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

March 15, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge