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pTK583

Plasmid

#114695

Purpose

SNAP-tagging at NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

SNAP

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTK587

Plasmid

#114692

Purpose

SNAP-tagging at p150's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

SNAP

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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kaiBC-

Plasmid

#101826

Purpose

deletes kaiBC locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

CmR knocking out kaiBC operon

Use

Cloning vector

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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C152

Plasmid

#174355

Purpose

Repair plasmid for introducing AID, T2A and mCherry at the C-teminus of human PABPC1

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Auxin-inducible degron (AID) and mCherry

Availability

Academic Institutions and Nonprofits only

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pGL3 RARE-RFP

Plasmid

#183054

Purpose

Fluorescent reporter for Retinoic Acid activity

Depositor

Chaya Kalcheim

Article

Rekler et al Elife. 2022 Apr 8;11. pii: 72723. doi: 1

Insert

RFP

Promoter

RARE, SV40

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-HaloTag7-mGFP

Plasmid

#184903

Purpose

Stable expression of HaloTag7-mGFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

monomeric EGFP

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-tTA

Plasmid

#104102

Purpose

Expresses tTA2 under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tTA2

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pQE31-rsEGFP2

Plasmid

#102879

Purpose

Expresses rsEGFP2 in bacterial cells

Depositor

Stefan Jakobs

Article

Grotjohann et al eLife. 2012 Dec 31:1:e00248. doi: 10.755

Insert

rsEGFP2

Tags

6xHisTag

Expression

Bacterial

Available Since

Jan. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-jAspSnFR3

Plasmid

#203488

Purpose

jAspSnFR3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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A1-20B1(P004)

Plasmid

#184056

Purpose

This plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.

Depositor

C. Ron Yu

Article

Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

Insert

A1-20B1 preamplifier

Use

Synthetic Biology

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTK630

Plasmid

#114697

Purpose

Express RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

RFP-Nano

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTK750

Plasmid

#114718

Purpose

Express mCherry-NuMA(5A-3) from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA 5A-3

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTK586

Plasmid

#114716

Purpose

SNAP-tagging at LGN's N-terminal locus (BSD)

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

SNAP

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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NK804

Plasmid

#166058

Purpose

Puromycin resistance and MRLC-mTFP expression for Salpingoeca rosetta

Depositor

Nicole King

Article

Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

Insert

MRLC-mTFP

Use

S. rosetta expression

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-Vph1-HaloTag

Plasmid

#140149

Purpose

Plasmid for pop-in/pop-out replacement of Vph1 with Vph1-HaloTag

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

VPH1

Expression

Yeast

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pTK503

Plasmid

#114717

Purpose

Express mCherry-NuMA(WT) from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pACS45

Plasmid

#204581

Purpose

GST-lamin A (389-646)

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

lamin A (389-646)

Tags

GST - lamin A (389-646)

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR1a-jAspSnFR3

Plasmid

#203460

Purpose

jAspSnFR3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3

Use

Gateway entry vector

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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YIplac211-Mup1-mScarlet2I

Plasmid

#140152

Purpose

Plasmid for pop-in/pop-out replacement of Mup1 with Mup1-mScarlet2I

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

MUP1

Expression

Yeast

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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NK802

Plasmid

#166056

Purpose

Puromycin resistance and mCherry expression for Salpingoeca rosetta

Depositor

Nicole King

Article

Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

Insert

Pac puromycin resistance gene

Use

S. rosetta expression

Tags

mCherry

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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