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JAK2 (V617F)-pcw107-V5

Plasmid

#64610

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

JAK2 (JAK2 Human)

Use

Lentiviral

Tags

V5

Mutation

V617F

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

Plasmid

#101060

Purpose

AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Publication

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has service

AAV1

Insert

CaMPARI2

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCMV-hAR

Plasmid

#89078

Purpose

mammalian expression of human androgen receptor in pCMV5

Depositor

Elizabeth Wilson

Publication

Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human androgen receptor (hAR) full-length (AR Human)

Expression

Mammalian

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pBABEpuro-gateway

Plasmid

#51070

Purpose

gateway adapted mammalian expression vector

Depositor

Matthew Meyerson

Publication

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pDEST-CMV-N-EGFP

Plasmid

#122842

Purpose

N-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLentiCas9-T2A-BFP

Plasmid

#78547

Purpose

Cas9 protein with BFP

Depositor

Roderic Guigo, Rory Johnson

Publication

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pET Trx His6 LIC cloning vector (2Tc-T)

Plasmid

#37239

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis and Thioredoxin (Trx)

Expression

Bacterial

Promoter

T7

Available since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Blast DEST (w524-1)

Plasmid

#19065

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pVITRO1-dV-IgG1/κ

Plasmid

#52213

Purpose

Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

Depositor

Andrew Beavil

Publication

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

Human immunoglobulin gamma 1 constant region
Human immunoglobulin kappa constant region

Expression

Mammalian

Mutation

Deleted Variable region

Promoter

Mouse Elongation Factor 1 Alpha and Rat Elongatio…

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET-Cas9-6xHis

Plasmid

#62374

Purpose

Expression of Cas9-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

Cas9_6xHis

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV-15F11-HA-mCh

Plasmid

#129591

Purpose

Encodes anti-HA scFv (anti-HA frankenbody) fused with mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Publication

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pcDNA3.2/v5-DEST hGlut1

Plasmid

#18085

Depositor

Wolf Frommer

Publication

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Glut1 (SLC2A1 Human)

Expression

Mammalian

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pK-myc-Par3b

Plasmid

#19388

Depositor

Ian Macara

Publication

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Partitioning-defective protein 3b (PARD3 Human)

Tags

Myc

Expression

Mammalian

Mutation

Full length, isoform b.

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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FU-tetO-Gateway

Plasmid

#43914

Depositor

John Gearhart

Publication

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

6xHis and V5

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLVX-UbC-rtTA-Ngn2:2A:EGFP

Plasmid

#127288

Purpose

all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

Depositor

Fred Gage

Publication

Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

Insert

Ngn2:2A:Ascl1 (NEUROG2 Human)

Use

Lentiviral

Tags

Ngn2 and EGFP linked via 2A

Expression

Mammalian

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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GFP-Ub KO.G76V

Plasmid

#11932

Depositor

Nico Dantuma

Publication

Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ubiquitin KO.G76V (RBX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Conjugation deficient mutant. All 7 lysines of ub…

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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MSCV PRDM16

Plasmid

#15504

Depositor

Bruce Spiegelman

Publication

Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

Insert

PRDM16 (isoform 4) (Prdm16 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Flag-HA-USP22

Plasmid

#22575

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP22 (USP22 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEF5B-FRT-GFP-αTAT1

Plasmid

#27099

Depositor

Maxence Nachury

Publication

Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

α-Tubulin K40 acetyltransferase1 (ATAT1 Human)

Tags

GFP, S-tag, and TEV site

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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E[beta]C

Plasmid

#24312

Depositor

Roel Nusse

Publication

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-betacatenin(deltaGSK) // SV40-mCherry (Ctnnb1 Mouse)

Use

Lentiviral

Mutation

Sites phosphorylated by GSK-3beta have been mutat…

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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