We narrowed to 4,062 results for: p2a

1. mKate2-P2A-APEX2-TAPP1-PH

   Plasmid

   #67662

   Purpose

   Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-TAPP1-PH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-ABE7.10-P2A-GFP

   Plasmid

   #162509

   Purpose

   Express ABE7.10-P2A-GFP by CMV promoter in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

   Insert

   ABE7.10-P2A-GFP
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-IDO2-p2a-eGFP

   Plasmid

   #187027

   Purpose

   Expresses IDO2 and GFP (seperately) in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

   Insert

   Indoleamine 2,3-dioxygenase 2 (IDO2 Human)
   

   Use

   Tags

   P2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. modRNAc0-ABE8e-P2A-GFP

   Plasmid

   #178177

   Purpose

   IVT template plasmid for making ABE8e-P2A-GFP modRNA

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

   Insert

   ABE8e
   

   Use

   Ivt template plasmid

   Tags

   P2A-GFP

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK445 (BSD-P2A-BromoTag)

   Plasmid

   #214375

   Purpose

   BromoTag for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BSD-P2A-BromoTag
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMMLV-mRFP1-P2A-Puro

   Plasmid

   #160228

   Purpose

   Expresses mRFP in mammalian cells

   Depositor

   Jason Sheltzer

   Article

   Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

   Insert

   mRFP1-P2A-Puro
   

   Use

   Retroviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-fDIO-mCherryP2ACre

   Plasmid

   #161773

   Purpose

   Flp-Dependent mCherry and Cre expression

   Depositor

   Baoji Xu

   Article

   An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

   Insert

   mCherry-P2A-Cre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-TO-dCas9-P2A-HSA

   Plasmid

   #121936

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   dCas9-P2A-HSA
   

   Use

   CRISPR and Lentiviral

   Tags

   HSA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-PGK-RAP2A-WT-neo

   Plasmid

   #156177

   Purpose

   Lentiviral vector for expression of RAP2A-WT, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   wild type form

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS613_pTetR-P2A-BFPnls/sgTelo

    Plasmid

    #108649

    Purpose

    Telomere-targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Telomere-targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFH2.12_GagZip-MCP-P2A-eGFP

    Plasmid

    #205527

    Purpose

    Express HIV GagZip-MCP-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    GagZip-MCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Nucleocapsid replaced with leucine zipper

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-CB-FLPe-P2A-tdTomato

    Plasmid

    #72259

    Purpose

    Express FLPe and tdTomato under CB promoter

    Depositor

    Kazuhiro Oka

    Insert

    FLPe-P2A-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T230I in tdTomato (please see depositor's co…

    Promoter

    CB promoter and chicken beta-actin + CMV enhancer

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-P2A-APEX2-ChBP

    Plasmid

    #108879

    Purpose

    Expression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    EGFP-P2A-APEX2-ChBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid

    #137730

    Purpose

    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA

    Depositor

    Fredrik Wermeling

    Article

    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKISox1-P2A-eGFP-Hygro

    Plasmid

    #115685

    Purpose

    Knock-in of P2A-eGFP at the mouse Sox1 gene

    Depositor

    David Suter

    Article

    Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

    Insert

    P2A-eGFP-loxP-PGK-Hygro-loxP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMGS54 (AID-P2A-Hygromycin)

    Plasmid

    #126583

    Purpose

    An AID-P2A-HygR cassette to tag the protein of interests with auxin-inducible degron at the c-terminal

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    AID-P2A-HygR
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-FNLSNG-P2A-Puro

    Plasmid

    #136900

    Purpose

    Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSNG
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

    Promoter

    EF1s

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVH64-Tier1-PSV40-Citrine_p2a_SEAP

    Plasmid

    #169558

    Purpose

    Tier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PSV40-driven citrine-P2A-SEAP fusion construct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PSV40

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3E-APEX2-P2A-EGFP

    Plasmid

    #108896

    Purpose

    Multisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    APEX2-P2A-EGFP
    

    Use

    Multisite gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. mKate2-P2A-APEX2-csGBP

    Plasmid

    #108875

    Purpose

    Expression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    mKate2-P2A-APEX2-csGBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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