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pSLQ11296: pHR-HSP-hyperdCas12a-miniVPR-EPS-puro

Plasmid

#210604

Purpose

heat shock promoter - activated hyperdCas12a-based transcriptional activator

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

hyperdCas12a-miniVPR

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

truncated hsp70

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pExpreS2-2

Plasmid

#175362

Purpose

G418R backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

Depositor

ExpreS2ion Biotechnologies

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Fused Actin-HSP70

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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DT12A

Plasmid

#80416

Purpose

Expresses 12 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBPhsFlp2::PEST

Plasmid

#63171

Purpose

Drosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock Promoter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

hsFlp2::PEST

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pRK010

Plasmid

#69874

Purpose

expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

Depositor

Georg Nagel, Robert Kittel

Article

Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

Insert

Channelrhodopsin-2

Use

Unspecified

Tags

Expression

Mutation

D156C (XXL)

Promoter

Drosophila Hsp70

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ11274:pHR-hU6-crTet-HSP-hyperdCas12a-miniVPR-CMV-mCherry

Plasmid

#210603

Purpose

heat shock promoter - activated hyperdCas12a-based transcriptional activator

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

hyperdCas12a-miniVPR

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

truncated hsp70

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pExpreS2-1

Plasmid

#175360

Purpose

ZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

Depositor

ExpreS2ion Biotechnologies

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Fused Actin-HSP70

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ11299: pHR-HSP-ABE8e-hyperdCas12a-EPS-puro

Plasmid

#210605

Purpose

heat shock promoter - activated hyperdCas12a-based transcriptional base editor

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

ABE8e-hyperdCas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

truncated hsp70

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pHRW-CRY2olig-VHH(GFP)

Plasmid

#126023

Purpose

express RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogenetics

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CRY2 PHRolig-VHH(GFP)

Use

Tags

RFP

Expression

Insect

Mutation

CRY2 E490G

Promoter

Hsp70

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pJFRC7-UAS-NPRR_ANP

Plasmid

#133243

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

rat atrial natriuretic peptide-fused codon-optimized GCaMP6s

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pHRW-CRY2-VHH(GFP)

Plasmid

#126022

Purpose

express RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogenetics

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CRY2 PHR-VHH(GFP)

Use

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS-hActin-luc2

Plasmid

#48081

Purpose

Luciferase Reporter with Gal4 DNA binding site upstream of hACTB promoter

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Luciferase

Use

Tags

Expression

Mammalian

Mutation

removed hsp70 promoter, inserted amplified 615 bp…

Promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pHW-CIBN-MP-HRW-CRY2-VHH(GFP)

Plasmid

#126024

Purpose

express RFP-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHR-VHH(GFP)

Use

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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HS-DN-Plk1

Plasmid

#105949

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Plk1 (PLK1 Human)

Use

Tags

Expression

Bacterial

Mutation

Dominant negative K82 R

Promoter

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHW-CIBN-MP-HFW-CRY2-VHH(GFP)

Plasmid

#126027

Purpose

express Flag-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHR-VHH(GFP)

Use

Tags

Flag

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLdSaCN

Plasmid

#123261

Purpose

Expresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Insert

gRNA and SaCas9

Use

CRISPR; Leishmania

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-WingGFP-tan

Plasmid

#83473

Purpose

EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

Depositor

Patricia Wittkopp

Article

Lamb et al Fly (Austin). 2016 Aug 5:1-12.

Insert

WingGFP-tan

Use

CRISPR

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Hsp70 and enhancer from D. melanogaster yellow ge…

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pDownstream1Lox

Plasmid

#164574

Purpose

Integration of 1 LoxN site in the genome of P. berghei

Depositor

Olivier Silvie

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Bidirectional PbEF1alpha promoter (PBANKA_1133300…

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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p3.2mar-GFP

Plasmid

#63694

Purpose

3.2 kb marine armor plate enhancer driving GFP reporter

Depositor

David Kingsley

Article

O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755

Insert

3.2 kb marine stickleback armor plate enhancer

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

hsp70

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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pUASTattB-GFP-Msp300KASH

Plasmid

#170806

Purpose

Expresses Msp300 KASH domain fused to GFP under Gal4/UAS control for Drosophila transgenesis

Depositor

Vikki Weake

Article

Jauregui-Lozano et al Genetics. 2021 May 22. pii: 6281219. doi

Insert

GFP-Msp300KASH

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

5xUAS + hsp70 minimal promoter

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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