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pCMV-HA-hIFITM2

Plasmid

#58398

Purpose

Expressed human IFITM2 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM2 (IFITM2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pET15-UbE2W

Plasmid

#15809

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2W (UBE2W Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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CLuc-FKBP

Plasmid

#31184

Depositor

David Piwnica-Worms

Article

Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

Insert

C-terminus of Firefly Luciferase and FK506-binding protein 12 (FKBP1A Human)

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pQCXIH-Myc-YAP-S94A

Plasmid

#33094

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

S94A

Promoter

CMV/MSV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pGL3 NSE promoter

Plasmid

#11606

Depositor

Rosalyn Adam

Article

Kim et al Endocrinology. 2004 Feb . 145(2):613-9.

Insert

NSE promoter (ENO2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pTALYM3B15

Plasmid

#47878

Purpose

TALE-mClover against mouse major satellite sequence

Depositor

Maria-Elena Torres-Padilla

Article

Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

Insert

TALE-mClover

Use

TALEN

Tags

mClover

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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HA-Cdk9

Plasmid

#28102

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Tags

3x HA

Expression

Bacterial and Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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human oct4-GFP

Plasmid

#21153

Depositor

Rudolf Jaenisch

Article

Green et al Nano Lett. 2008 Oct . 8(10):3126-30.

Insert

hOCT4-GFP (POU5F1 Human)

Use

Human targeting

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry

Plasmid

#78534

Purpose

Backbone to clone single or paired sgRNAs

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA)

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ420 - Peft-3::GFP::H2B (ubiquitous)

Plasmid

#34877

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

Peft-3 GFP H2B tbb-2 UTR

Use

Worm targeting

Mutation

S65C

Promoter

eef-1A.1 (eft-3)

Available Since

Feb. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pFast-FT-N1

Plasmid

#31910

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Fast-FT

Expression

Mammalian

Mutation

M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-ABI-dCas9-P2A-mcherry

Plasmid

#121513

Purpose

expression of AB1-fused-dcas9 plasmids

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

dCas9

Use

Lentiviral

Tags

AB1 and P2A-mcherry

Expression

Mammalian

Promoter

PGK

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-PDGFRA

Plasmid

#23892

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRA (PDGFRA Human)

Use

Gateway donor vector

Mutation

M260I

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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p212 pCMV-EGFP/RFP

Plasmid

#8389

Purpose

Gene-switch Cre reporter plasmid with floxed EGFP gene upstream of RFP.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP

Use

Cre/Lox

Expression

Mammalian

Available Since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

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pDONR223-ABL1

Plasmid

#23939

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ABL1 (ABL1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pWPXLd

Plasmid

#12258

Depositor

Didier Trono

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag Runx1 FL (P#1790)

Plasmid

#14585

Depositor

Matija Peterlin

Article

Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

Insert

Runx1 (Runx1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pKSI-1

Plasmid

#51725

Purpose

cloning backbone

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Xist-PA

Plasmid

#26760

Depositor

Rudolf Jaenisch

Article

Wutz et al Mol Cell. 2000 Apr . 5(4):695-705.

Insert

Xist (Xist Mouse)

Expression

Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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mTOR_1 shRNA

Plasmid

#1855

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

mTOR (MTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 23, 2005

Availability

Academic Institutions and Nonprofits only

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