59,541 results

1. TFORF3095

   Plasmid

   #144571

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   LIN9 (LIN9 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLexM-SLC7A11

   Plasmid

   #197993

   Purpose

   Expresses SLC7A11 with an N-terminal FLAG tag in mammalian cells

   Depositor

   Simon Newstead

   Article

   Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

   Insert

   SLC7A11 (SLC7A11 Human)
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mEmerald-Integrin-Beta1-N-18

   Plasmid

   #54129

   Purpose

   Localization: Integrin/Focal Adhesions, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Integrin-Beta1 (ITGB1 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. MSCV-(mcs) human CSF1 full length-IRES-Puro

   Plasmid

   #86797

   Purpose

   retroviral expression of CSF-1

   Depositor

   Martine Roussel, Charles Sherr

   Article

   Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

   Insert

   CSF-1 (CSF1 Human)
   

   Use

   Retroviral

   Tags

   IRES-Puro

   Expression

   Mammalian

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCE-K2N

   Plasmid

   #154879

   Purpose

   Episomally expresses KLF2 and NANOG in mammalian cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

   Insert

   KLF2-2A-NANOG (KLF2, NANOG Human)
   

   Expression

   Mammalian

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Desmoplakin I tension sensor (F40-based)

   Plasmid

   #119186

   Purpose

   The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. plenti-CAG-IRF4-FLAG-IRES-GFP

   Plasmid

   #107389

   Purpose

   Lentiviral expression of IRF4-FLAG

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IRF4 (IRF4 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. IDG_CHRNA2_OE_1

   Plasmid

   #161648

   Purpose

   Overexpresses CHRNA2

   Depositor

   Mike McManus

   Insert

   CHRNA2 (CHRNA2 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ADRA2B-Tango

   Plasmid

   #66217

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ADRA2B (ADRA2B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

10. mEmerald-Keratin-17

    Plasmid

    #54134

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

    Insert

    Keratin (KRT18 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pDEST-FRT/TO-3xFLAG-REV7

    Plasmid

    #114127

    Purpose

    Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    REV7 (MAD2L2 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV/TetO2

    Available Since

    Aug. 16, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. TFORF1920

    Plasmid

    #142887

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF1682

    Plasmid

    #142825

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SIM1 (SIM1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP VAMP7 (1-120)

    Plasmid

    #42317

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Longin domain (1-120) (VAMP7 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Δ120-220

    Promoter

    CMV

    Available Since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pH6HTC_FAM98A-Halo

    Plasmid

    #175329

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    FAM98A (FAM98A Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET30-2-GPD1L

    Plasmid

    #83912

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP SMG1 WT

    Plasmid

    #199571

    Purpose

    Expresses WT SMG1 fused to GFP

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    SMG1 WT (SMG1 Human)
    

    Tags

    GFP fusion protein

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Human RNF149-3X FLAG

    Plasmid

    #225332

    Purpose

    Lentiviral expression of human RNF149 with 3x FLAG tag in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

    Insert

    RNF149 (RNF149 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-CMV-Blast-DNTGFBR2-HA

    Plasmid

    #130888

    Purpose

    Expresses dominant negative mutant human TGFBR2

    Depositor

    Gian-Paolo Dotto

    Article

    Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

    Insert

    TGFBR2 (TGFBR2 Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Dominant Negative receptor lacking cytosolic cata…

    Promoter

    CMV

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223-CAMK2B

    Plasmid

    #23820

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CAMK2B (CAMK2B Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only