We narrowed to 16,513 results for: por
-
Plasmid#238338PurposeproUBI10::VIH2-FL::FLAG-P378V-G500V-G501A ubiquitous expression in Arabidopsis plantsDepositorInsertVIP1 (VIP1 Mustard Weed)
UseTags3x Flag-tagExpressionPlantMutationAtVIH2-P378V-G500V-G501APromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
proUBI10::VIH2-FL::FLAG-R722S-D724A-T726A
Plasmid#238339PurposeproUBI10::VIH2-FL::FLAG-R722S-D724A-T726A ubiquitous expression in Arabidopsis plantsDepositorInsertVIP1 (VIP1 Mustard Weed)
UseTags3x Flag-tagExpressionPlantMutationAtVIH2-R722S-D724A-T726APromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
proUBI10::VIH2-FL::FLAG-K383A-K447A-K598A-R722A
Plasmid#238340PurposeproUBI10::VIH2-FL::FLAG-K383A-K447A-K598A-R722A ubiquitous expression in Arabidopsis plantsDepositorInsertVIP1 (VIP1 Mustard Weed)
UseTags3x Flag-tagExpressionPlantMutationAtVIH2-K383A-K447A-K598A-R722APromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A
Plasmid#238334PurposeproUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A ubiquitous expression in Arabidopsis plantsDepositorInsertVIP1 (VIP1 Mustard Weed)
UseTags3x Flag-tagExpressionPlantMutationAtVIH2-R372A-H373A-R376A-E882APromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
proUBI10::VIH2-FL::FLAG-K379A-K381A-K498A
Plasmid#238341PurposeproUBI10::VIH2-FL::FLAG-K379A-K381A-K498A ubiquitous expression in Arabidopsis plantsDepositorInsertVIP1 (VIP1 Mustard Weed)
UseTags3x Flag-tagExpressionPlantMutationAtVIH2-K379A-K381A-K498APromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
mScarlet-LRRK1 K746G
Plasmid#233586PurposeExpresses LRRK1 K746G in mammalian cellsDepositorInsertLRRK1 K746G (LRRK1 Human)
UseTagsmScarletExpressionMammalianMutationPromoterAvailable sinceJune 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR2b-PTPN11(D425A,C459S)
Plasmid#215515PurposeGateway entry vector with a phosphatase-inactive Shp2 mutant (D425A,C459S)DepositorInsertPTPN11 (PTPN11 Human)
UseGateway entry vectorTagsExpressionMutationD425A, C459S (Phosphatase inactive)PromoternoneAvailable sinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR2b-ITGB1(WT)
Plasmid#215450PurposeGateway entry vector with integrin beta1 wild-type (WT)DepositorInsertITGB1 (ITGB1 Human)
UseGateway entry vectorTagsExpressionMutationSilent mutations added to disrupt shRNA binding a…PromoternoneAvailable sinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV.tetO.Nurr1
Plasmid#234848Purpose3rd generation lentiviral vector, expresses Nurr1 under control of TetON promoterDepositorInsertNurr1 (Nr4a2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH
Plasmid#235266PurposeComMAND EGFP-FMRP open-loop circuitDepositorInsertEGFP-Fmr1 (Fmr1 Mouse)
UseSynthetic BiologyTagsFLAGExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH
Plasmid#235267PurposeComMAND EGFP-FMRP closed-loop circuitDepositorInsertEGFP-Fmr1 (Fmr1 Mouse)
UseSynthetic BiologyTagsFLAGExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
SSH-pSUMO-β′-COPI-WD40-Lys17Ala
Plasmid#208171PurposeBacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Lys17Ala mutant (residues 1-301)DepositorInsertN-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Lys17Ala substitution
UseTagsHis-tag, SUMO-tag, and Strep-tagExpressionBacterialMutationLys17Ala substitutionPromoterAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAED2
Plasmid#234599PurposeFluorescent reporter of the SOS responseDepositorInsertsmScarlet-I
gfp-mut2
UseReporterTagsExpressionBacterialMutationGFPmut2 was derived from avGFP with the following…PromoterPtet+dnaK P1 and cdaAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
BD-pGEX5X3-BD-SARS-CoV-2-S-tail-WT
Plasmid#218455PurposeBacterial expression plasmid for the SARS-CoV-2 cytosolic tail with an N-terminal GST tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying an N-terminal GST-tag (S SARS-CoV-2)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
MPC1-AA-FLAG
Plasmid#228904PurposeExpression of mouse MPC1 in which K45 and K46 motif sites were replaced with alanine blocking acetylation with a C terminal FLAG tagDepositorInsertMPC1 (Mpc1 Mouse)
UseTagsFLAGExpressionMammalianMutationK45 and K46 motif sites were both replaced with a…PromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-mTagBFP2-rMPC1 shRNA
Plasmid#229016PurposeExpression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfectionDepositorInsertMPC1 (Mpc1 Rat)
UseLentiviralTagsmTagBFP2ExpressionMammalianMutationPromoterU6Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 2xHA LTB4R
Plasmid#226810PurposeTet-inducible lentiviral vector for 2xHA LTB4R expressionDepositorInsertLTB4R (LTB4R Human)
UseLentiviralTags2xHA tag followed by a GDPPVAT linkerExpressionMutationSequence optimized from NP_858043.1PromoterAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A 2xHA GPR153
Plasmid#226808PurposeGateway entry vector for 2xHA GPR153DepositorInsertGPR153 (GPR153 Human)
UseGateway entryTags2xHA tag followed by a GDPPVAT linkerExpressionMutationSequence optimized from NP_997253.2PromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A 2xHA LTB4R
Plasmid#226806PurposeGateway entry vector for 2xHA LTB4RDepositorInsertLTB4R (LTB4R Human)
UseGateway entryTags2xHA tag followed by a GDPPVAT linkerExpressionMutationSequence optimized from NP_858043.1PromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 2xHA GPR137
Plasmid#226813PurposeTet-inducible lentiviral vector for 2xHA GPR137 expressionDepositorInsertGPR137 (GPR137 Human)
UseLentiviralTags2xHA tag followed by a GDPPVAT linkerExpressionMutationSequence optimized from NP_001164351PromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.CBX8ΔChromo
Plasmid#224708PurposeExpresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tagDepositorInsertCBX8 chromodomain truncation (CBX8 Human)
UseTagsHMBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.mEGFP-CBX8
Plasmid#224710PurposeExpresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.DepositorInsertCBX8 (CBX8 Human)
UseTagsmEGFPExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFB1.HMBP.PrS.CBX8-KR21A
Plasmid#224709PurposeExpresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertCBX8 KR21A mt (CBX8 Human)
UseTagsHMBPExpressionInsectMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.CBX8
Plasmid#224707PurposeExpresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertCBX8 (CBX8 Human)
UseTagsHMBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-cDELE1(142-515)-sumo
Plasmid#226107PurposecDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assaysDepositorInsertDELE1 (DELE1 Human)
UseTagsSumoExpressionMammalianMutationencodes only residues 142-515 of DELE1 (represent…PromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-cDELE1(142-515)-GFP-IRES-mCherry
Plasmid#226102PurposecDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cellsDepositorInsertDELE1 (DELE1 Human)
UseTagsGFPExpressionMammalianMutationonly encodes for residues 142-515 of DELE1 corres…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-SLC66A1_STOP
Plasmid#221418PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC66A1 (PQLC2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-SLC66A5_STOP
Plasmid#221422PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC66A5 (MPDU1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-MPP1
Plasmid#221428PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertMPP1 (MPP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala
Plasmid#208172PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
UseTagsStrep-tagExpressionMammalianMutationArg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…PromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only