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Showing: 5981 - 6000 of 6203 results

  6. pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174646
    Purpose
    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…
    Promoter
    CMV (with TetOn)
    Available Since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174646-plasmid-map-sequence-id-346959

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  7. pB80-KIF1A(1-365)-mVenus-SSPB(micro)

    Plasmid
    #174635
    Purpose
    Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…
    Promoter
    Chicken beta-actin
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174635-plasmid-map-sequence-id-346993

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  8. pB80-KIF1A(1-383)-GFP-SSPB(micro)

    Plasmid
    #174627
    Purpose
    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-383)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…
    Promoter
    Chicken beta-actin
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174627-plasmid-map-sequence-id-346971

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  14. pFUSEss-CHIg-mG3_CH2charge

    Plasmid
    #105858
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG3 heavy chain (Ighg3 Mouse)
    Expression
    Mammalian
    Mutation
    mutated heavy chain of mouse IgG3, muatations: Hi…
    Promoter
    hEF1-HTLV prom
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    105858-plasmid-map-sequence-id-206030

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  15. pMCh-623

    Plasmid
    #92266
    Purpose
    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cells
    Depositor
    Marina Chekulaeva, Witold Filipowicz
    Article
    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1
    Insert
    dGW182 6W6A (gw Fly)
    Tags
    NHA
    Expression
    Insect
    Mutation
    The following point mutations were introduced W23…
    Promoter
    Ac5
    Available Since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92266-plasmid-map-sequence-id-194528

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  18. EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

    Plasmid
    #198753
    Purpose
    Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a
    Available Since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198753-plasmid-map-sequence-id-398000

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  20. MSCV-opEBNA2-IRES-GFP

    Plasmid
    #220354
    Purpose
    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)
    Depositor
    Klaus Rajewsky
    Article
    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d
    Insert
    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    Use
    Retroviral
    Tags
    3 x HA tag
    Expression
    Mammalian
    Mutation
    codon-optimised for expression in mammalian cells
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    220354-plasmid-map-sequence-id-437068

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Showing: 5981 - 6000 of 6203 results