We narrowed to 31,069 results for: LIS;

1. pYPQ230-RVQ

   Plasmid

   #200187

   Purpose

   Gateway entry clone (attL1 & attR5) for LbCas12a-RVQ mediated genome editing

   Depositor

   Yiping Qi

   Article

   Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

   Insert

   LbCas12a-RVQ
   

   Use

   CRISPR; Gateway compatible lbcas12a-rv entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   G146R, R182V, E795Q

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. G1BgSVNA-CDK4

   Plasmid

   #16573

   Depositor

   Bert Vogelstein

   Article

   Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pET21-pelB-LaG-21

   Plasmid

   #172749

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-21
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21-pelB-LaG-37

   Plasmid

   #172756

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-37
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTR223-BMRF2

   Plasmid

   #195803

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BMRF2

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BMRF2 (BMRF2 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR223-BKRF2

   Plasmid

   #195773

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BKRF2

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BKRF2 (BKRF2 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21-pelB-LaM-1

   Plasmid

   #172767

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-1
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pICSL11055

   Plasmid

   #68252

   Purpose

   Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-eBE-S3

   Plasmid

   #101093

   Purpose

   Expresses eBE-S3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

   Insert

   eBE-S3
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21-pelB-LaG-29

    Plasmid

    #172753

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-29
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FH-TET3-HxDmut-pEF

    Plasmid

    #127896

    Purpose

    Expresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079A

    Depositor

    Patrick Hogan, Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    TET3-HxDmut (TET3 Human)
    

    Tags

    HA and Flag

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR223-BGLF4

    Plasmid

    #195811

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BGLF4

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BGLF4 (BGLF4 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. px330-UFL1 sgRNA2

    Plasmid

    #134636

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA2 (UFL1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3-V5-PTPN14-PPxY

    Plasmid

    #61006

    Purpose

    Expresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tag

    Depositor

    Jianmin Zhang

    Article

    Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

    Insert

    PTPN14 PPxY mutation (PTPN14 Human)
    

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    PPxY mutation

    Promoter

    CMV

    Available Since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

15. VVT1

    Plasmid

    #65779

    Purpose

    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SaCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hTRAFD1-pCMV14-3xFlag

    Plasmid

    #73587

    Purpose

    Express human TRAFD1 (FLN29) in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

    Insert

    TRAFD1 (TRAFD1 Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    The original vector hTRAFD1-mCMV14-Flag was a gif…

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMV-HIS3

    Plasmid

    #65333

    Purpose

    standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUs

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    HIS3
    

    Use

    Synthetic Biology

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV6- Entry-RPL26DC-DDK

    Plasmid

    #133867

    Purpose

    Expression of C-terminally FLAG-tagged human RPL26 mutant lacking the C-terminal UFMylation sites

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    RPL26DC-myc-FLAG (RPL26 Human)
    

    Tags

    Myc-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lox-stop-Lox p53 R270H

    Plasmid

    #14853

    Depositor

    Tyler Jacks

    Article

    Olive et al Cell. 2004 Dec 17. 119(6):847-60.

    Insert

    p53 R270H (Trp53 Mouse)
    

    Use

    Cre/Lox

    Tags

    Lox-STOP-lox

    Expression

    Mammalian

    Mutation

    Contact mutant R270H. (Murine p53 codon 270 corre…

    Available Since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

20. px330-UFL1 sgRNA1

    Plasmid

    #134635

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA1 (UFL1 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge