We narrowed to 10,611 results for: t7

1. Sc eRF3∆165N (WT)

   Plasmid

   #175418

   Purpose

   Encodes His-MBP-TEV-WT eRF3∆165N

   Depositor

   Joseph Puglisi

   Article

   Lawson et al Science. 2021 Aug 20;373(6557):876-882.

   Insert

   eRF3 (SUP35 Budding Yeast)
   

   Tags

   His-MBP-TEV

   Expression

   Bacterial

   Mutation

   ∆165N

   Promoter

   T7

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET30b jGCaMP8f F366H

   Plasmid

   #204139

   Purpose

   Production of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f F366H
   

   Tags

   N-terminal His tag

   Expression

   Bacterial

   Mutation

   Phenylalanine 366 changed to histidine

   Promoter

   T7

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Fast-GCaMP-EF16

   Plasmid

   #52642

   Purpose

   genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

   Depositor

   Samuel Wang

   Article

   Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

   Insert

   Fast-GCaMP-EF16
   

   Expression

   Mammalian

   Mutation

   D431N/D433N/D435N/E442A mutated from GCaMP3

   Promoter

   T7

   Available Since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

4. CL7-LbCas12aD156R

   Plasmid

   #164659

   Purpose

   Bacterial expression of mutated LbCas12aD156R using CL7/Im7

   Depositor

   Piyush Jain

   Article

   Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

   Insert

   LbCas12a-D156R
   

   Tags

   Thioredoxin, CL7, SUMO

   Expression

   Bacterial

   Mutation

   changed Aspartic Acid 156 to Arginine

   Promoter

   T7

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. RP1B-PP1alpha

   Plasmid

   #51768

   Purpose

   Expresses PP1 alpha residues 7-330

   Depositor

   Wolfgang Peti

   Article

   Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

   Insert

   pp1 alpha
   

   Tags

   His, TEV protease site, and Thio-6

   Expression

   Bacterial

   Mutation

   Contains PP1 alpha residues 7-330

   Promoter

   T7-lac

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCellFree_G03 IL1B

   Plasmid

   #67066

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   IL1B (IL1B Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. Fast-GCaMP-EF19

   Plasmid

   #52644

   Purpose

   genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

   Depositor

   Samuel Wang

   Article

   Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

   Insert

   Fast-GCaMP-EF19
   

   Expression

   Mammalian

   Mutation

   T328D/T330D mutated from GCaMP3

   Promoter

   T7

   Available Since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-Pert Heavy chain-SpyTag003

   Plasmid

   #216309

   Purpose

   Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   H2-18 Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

   Plasmid

   #216292

   Purpose

   Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Tras heavy chain-GSG-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNP1 sfGFP trigger

    Plasmid

    #107359

    Purpose

    Trigger sequence for pNP1 sfGFP toehold switch

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold trigger 7
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDuet-1_DARPinOFF7

    Plasmid

    #182396

    Purpose

    Bacterial expression of DARPinOFF7

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    DARPinOFF7
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. RanBP2-RB3-4-pET23a

    Plasmid

    #85769

    Purpose

    bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag

    Depositor

    Frauke Melchior

    Article

    Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

    Insert

    RanBP2 fragment (RANBP2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    fragment (amino acids 2304-3062)

    Promoter

    T7

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-flag-mKdm4d(H189A)-polyA

    Plasmid

    #61554

    Purpose

    For mRNA synthesis or expression in mammalian cells

    Depositor

    Yi Zhang

    Article

    Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

    Insert

    Kdm4d (catalytic mutant)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    changed Histidine 189 (of Kdm4d) to Alanine

    Promoter

    CMV and T7

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET151-D-TOPO-PARP14-SΔK

    Plasmid

    #216658

    Purpose

    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

    Depositor

    Herwig Schüler

    Article

    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

    Insert

    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    Deletion starting from Q1390 to D1448 and substit…

    Promoter

    T7

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSG5-p110α-WT

    Plasmid

    #55709

    Purpose

    eukaryotic expression of Flag tagged p110a WT

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p110 alpha
    

    Tags

    flag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. dFP-mini

    Plasmid

    #120543

    Purpose

    Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

    Depositor

    Brian Chow

    Article

    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

    Insert

    de novo fluorescence-activating protein mini (dFP-mini)
    

    Tags

    6xHis - TEV cleavage

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCellFree_G03 CALR

    Plasmid

    #67086

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CALR (CALR Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pT7C-HSPH1

    Plasmid

    #177662

    Purpose

    Recombinant protein synthesis of human HSPH1

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSPH1 (HSPH1 Human)
    

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. DUSP6

    Plasmid

    #40772

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

    Insert

    DUSP6 (DUSP6 Human)
    

    Tags

    6His and TEV

    Expression

    Bacterial

    Mutation

    Starting Met removed

    Promoter

    T7

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pTRCHisB-TRF2∆M

    Plasmid

    #50489

    Purpose

    E. Coli N-term HexHis Tagged Protein Expression Vector

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TRF2 Delta Myb (TERF2 Human)
    

    Tags

    6x His, T7 tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Contains amino acids 3-437

    Promoter

    pTrc

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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