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Showing: 6001 - 6020 of 8366 results

  3. pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

    Plasmid
    #228530
    Purpose
    Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.
    Depositor
    Roman Jerala
    Article
    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80
    Insert
    myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Synthetic, Photinus pyralis, Human)
    Tags
    HA and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    228530-plasmid-map-sequence-id-456690

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  6. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(deltaI)

    Plasmid
    #204701
    Purpose
    This plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.
    Depositor
    Michael Rosen
    Article
    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1
    Insert
    BRD4-p300(deltaI) (BRD4, EP300 Human)
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    deletion of intrinsically disordered regions of p…
    Promoter
    CMV
    Available Since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    204701-plasmid-map-sequence-id-409083

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  7. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(I)

    Plasmid
    #204700
    Purpose
    This plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.
    Depositor
    Michael Rosen
    Article
    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1
    Insert
    BRD4-p300(I) (BRD4, EP300 Human)
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    deletion of all TF-bidning domains and HAT in p300
    Promoter
    CMV
    Available Since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204700-plasmid-map-sequence-id-405170

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  8. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(HI)

    Plasmid
    #204699
    Purpose
    This plasmid allows for inducible expression of engineered BRD4-p300(HI) fusion tagged with mNeonGreen, in mammalian cells.
    Depositor
    Michael Rosen
    Article
    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1
    Insert
    BRD4-p300 (BRD4, EP300 Human)
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    deletion of all TF-bidning domains in p300
    Promoter
    CMV
    Available Since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204699-plasmid-map-sequence-id-405091

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  13. pD649-HAsp-ADGRF5-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157019
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    ADGRF5 (ADGRF5 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    157019-plasmid-map-sequence-id-303147

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  14. pD649-HAsp-IL18R1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156749
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    IL18R1 (IL18R1 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156749-plasmid-map-sequence-id-302607

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  15. pD649-HAsp-FCGR1B-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156588
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    FCGR1B (FCGR1B Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156588-plasmid-map-sequence-id-302285

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  17. pGEX2T-TEV CHMP1B 186-199 L188A/L192A

    Plasmid
    #108288
    Purpose
    Expresses residues 186-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-286
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Available Since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108288-plasmid-map-sequence-id-245273

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  18. pGEX2T-TEV CHMP1B 186-199 L192A/L195A

    Plasmid
    #108290
    Purpose
    Expresses residues 186-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-287
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Available Since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108290-plasmid-map-sequence-id-245275

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  19. pTW5126

    Plasmid
    #129732
    Purpose
    polyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA protein
    Depositor
    James Fleckenstein
    Article
    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do
    Insert
    eatA-DUF::His8
    Tags
    9His tag
    Expression
    Bacterial
    Mutation
    replaces the domain of unknown function correspon…
    Promoter
    araBAD
    Available Since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    129732-plasmid-map-sequence-id-257612

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  20. B9-COMP-blac-flag-his

    Plasmid
    #111020
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    6-cysteine protein (B9) (PF3D7_0317100 )
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    111020-plasmid-map-sequence-id-224595

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Showing: 6001 - 6020 of 8366 results