We narrowed to 13,291 results for: cDNA

1. (137) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4)

   Plasmid

   #160740

   Purpose

   Expresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobody

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   HA-nEPEA-(EAAAK)4-OGT(4)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-dCas9-MQ1(C141S)-EGFP

   Plasmid

   #89635

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR

   Tags

   3*FLAG, 6*His, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   C141S, TGC-->TCC

   Promoter

   CMV

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO/Flag-tGFP-HuR.wt

   Plasmid

   #110376

   Purpose

   Mammalian expression of turboGFP-HuR (wild type) with FLAG tag, Flp-In T-Rex system

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Tags

   FLAG and tGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 LIC 6A HIS-TEV-DPP8

   Plasmid

   #166793

   Purpose

   Mammalian cell expression vector for Flag-tagged DPP8

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP8 (DPP8 Human)
   

   Use

   Tags

   HIS-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nano-lantern-plasma membrane/pcDNA3

   Plasmid

   #51975

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   HIS and Lyn

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCDNA3 5'cMyc-SMCl- S957A/S966A

   Plasmid

   #32372

   Depositor

   Michael Kastan

   Article

   Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

   Insert

   SMC1A (SMC1A Human)
   

   Use

   Tags

   c-Myc

   Expression

   Mammalian

   Mutation

   S957A/S996A

   Promoter

   Available Since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1-tvD-Cit-tevs-DHFR

   Plasmid

   #116054

   Purpose

   Reporter plasmid for measuring protease activity

   Depositor

   Michael Elowitz

   Article

   Gao et al Science. 2018 Sep 21;361(6408):1252-1258

   Insert

   tvD-Cit-tevs-DHFR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-myc3-CUL4A delta N52

   Plasmid

   #19952

   Depositor

   Yue Xiong

   Article

   Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

   Insert

   CUL4A (cullin 4A) (CUL4A Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   Missing 52 residues from N terminal CUL4A.

   Promoter

   Available Since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5 FRT TO Myc ArB WT RNAiR

   Plasmid

   #59806

   Purpose

   Allows the integration of Myc ArB in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

   Insert

   Aurora B (AURKB Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   RNAi resistant

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO SBBP-CDK1 WT (2007)

    Plasmid

    #61847

    Purpose

    mammalian expression of CDK1 WT, used for Ips

    Depositor

    Jonathon Pines

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Tags

    SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCDNA3 Flag p38 gamma (agf)

    Plasmid

    #20354

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 gamma(agf) (Mapk12 Rat)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Replace Thr 183 and Tyr 185 with Ala and Phe resp…

    Promoter

    Available Since

    April 29, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3 Flag-MBD1 R44Q-14-mVenusC

    Plasmid

    #191394

    Purpose

    R44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    pcDNA3 Flag-MBD1 R44Q-14-mVenusC
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R44Q

    Promoter

    CMV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Flag mTOR S2035T R2109A

    Plasmid

    #26606

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S2035T R2109A

    Promoter

    Available Since

    Nov. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

    Plasmid

    #181853

    Purpose

    mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Myc3-Cul3 H2M/H5M

    Plasmid

    #21591

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    cullin 3 (CUL3 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Mutated residues H2M/H5M

    Promoter

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1 SARS-CoV-2 S D614-trunc

    Plasmid

    #158076

    Purpose

    Expresses SARS-CoV-2 spike protein with a truncation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1/CIP2A(1-905) R522D V5 His

    Plasmid

    #119289

    Purpose

    CIP2A R522D V5 overexpression, CIP2A dimer defective mutant

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Use

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    R522D

    Promoter

    T7

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-Cas9-T2A-STOP-TdT

    Plasmid

    #126425

    Purpose

    Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-T2A-STOP-TdT (DNTT Human, Streptococcus pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alanine-6 of TdT has been changed to a STOP codon

    Promoter

    pCMV

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 LIC 6A CARD8-FLAG S297A

    Plasmid

    #169949

    Purpose

    Mammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S297A

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Nano-lantern(Ca2+)_300/pcDNA3

    Plasmid

    #51983

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-3GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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