We narrowed to 10,611 results for: t7

1. pET-28a-His6-SUMO-VAMP3

   Plasmid

   #135552

   Purpose

   Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP3
   

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G06_ZIKV NS5

   Plasmid

   #145358

   Purpose

   Cell-free expression vector for Zika virus NS5 protein with C-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS5
   

   Tags

   mCherry and 8xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCSG28-SH3-588

   Plasmid

   #185920

   Purpose

   Expresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferase

   Depositor

   Brian Kuhlman

   Article

   Park et al Nat Chem Biol. 2023 Apr;19(4):460-467. d

   Insert

   SH3-588
   

   Tags

   6x HIS tag

   Expression

   Bacterial

   Mutation

   Designed enzyme with novel interface between huma…

   Promoter

   T7

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2CT-His-MBP-Lbu_C2c2_R1079A

   Plasmid

   #83494

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R1079A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R1079A

   Promoter

   T7

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-NtLAtC60αβC20

   Plasmid

   #160873

   Purpose

   Expression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcL, cpn60α, cpn60β, cpn20
   

   Expression

   Bacterial

   Promoter

   rbcL: pBAD, chaperones: T7

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-kcnk3a

   Plasmid

   #208985

   Purpose

   Riboprobe generation

   Depositor

   GuangJun Zhang

   Article

   Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

   Insert

   kcnk3a (kcnk3a Zebrafish)
   

   Use

   Cloning

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET29b-eGFP-dspB(E184Q)-6xHis

   Plasmid

   #175783

   Purpose

   Plasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Tags

   Hexahistidine tag and eGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE80l A-CC-A

   Plasmid

   #196478

   Purpose

   Protein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELP

   Depositor

   Fei Sun

   Article

   Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

   Insert

   A-CC-A
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TAL2431

   Plasmid

   #36830

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   HDAC2-TALEN_Right (Hdac2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pmCellFree_KA1_ZIKVC

    Plasmid

    #145339

    Purpose

    Cell-free/mammalian expression vector for Zika virus capsid protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV Capsid
    

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Promoter

    T7- CMV/CAG

    Available Since

    April 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7C-DNAJB4

    Plasmid

    #177656

    Purpose

    Recombinant protein synthesis of human DNAJB4

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    DNAJB4 (DNAJB4 Human)
    

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-RABIF-FLAG

    Plasmid

    #135558

    Purpose

    Express human RABIF in mammalian cells, FLAG at N terminal

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    RABIF (RABIF Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    T7 promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pThuS

    Plasmid

    #171674

    Purpose

    ThuS expression in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

    Insert

    Glycosyltransferase ThuS
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-R4-uAb

    Plasmid

    #101801

    Purpose

    Bacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

    Depositor

    Matthew DeLisa

    Article

    Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

    Insert

    R4-uAb
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. TAL2302

    Plasmid

    #36731

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BRCA2-TALEN_Left (Brca2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

16. TAL2303

    Plasmid

    #36732

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BRCA2-TALEN_Right (Brca2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pET28a-ybbR-HIS-sfGFP-DocI

    Plasmid

    #58708

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    sfGFP-DocI
    

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

    Plasmid

    #87350

    Purpose

    S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/S355C/C574S/S867C

    Promoter

    T7

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. hisSUMO-ZFP57

    Plasmid

    #102716

    Purpose

    Express DNA binding domain of human Zinc finger protein 57 in E. coli

    Depositor

    Gary Stormo

    Article

    Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

    Insert

    Zinc finger protein 57 (ZNF57 Human)
    

    Tags

    hisSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. PsaA

    Plasmid

    #159997

    Purpose

    Expresses Streptococcus pneumoniae PsaA (19-309) in bacterial cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    PsaA (19-309)
    

    Tags

    His6-tag and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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