We narrowed to 7,668 results for: CCH

1. pPMVAd36

   Plasmid

   #98302

   Purpose

   Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   PCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

   Expression

   Yeast

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUpstream2Lox

   Plasmid

   #164573

   Purpose

   Integration of 2 LoxN sites in the genome of P. berghei

   Depositor

   Olivier Silvie

   Inserts

   Green fluorescent protein - human dihydrofolate reductase (GFP-2A-hDHFR) (DHFR Synthetic, Human)
   bifunctional yeast enzyme cytosine deaminase and uridyl phosphoribosyl transferase (yFCU) (FCY1, FUR1 Budding Yeast)
   

   Use

   Cre/Lox

   Promoter

   Bidirectional PbEF1alpha promoter (PBANKA_1133300…

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. p413TEF-SirT1

   Plasmid

   #51746

   Purpose

   overexpression of human SirT1 in S. cerevisiae, HIS3 marker

   Depositor

   Markus Ralser

   Article

   Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

   Insert

   SirT1 (SIRT1 Human)
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. prp8-G736E

   Plasmid

   #108450

   Purpose

   prp8-G736E in pRS313 backbone for expression in yeast

   Depositor

   Christine Guthrie

   Article

   Mayerle et al Proc Natl Acad Sci U S A. 2019 Feb 5;116

   Insert

   PRP8 (PRP8 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   changed G736 to E

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB3035(X-4 MarkerFree)

   Plasmid

   #73273

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB700 lenti gRNA KanR neo

   Plasmid

   #167909

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. BAS285 C terminal mKO2 hygBR

   Plasmid

   #74080

   Purpose

   S.pombe recoded mKO2 in universal C-terminal tagging plasmid, hygB resistance.

   Depositor

   Bassem Al-Sady

   Article

   Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

   Insert

   mKO2
   

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

8. hSyn-GW-dCas9-SET2-IRES2-mCherry

   Plasmid

   #202089

   Purpose

   Neuron-specific expression vector of dCas9-Set2

   Depositor

   Elizabeth Heller

   Article

   Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

   Insert

   dCas9-Set2
   

   Tags

   3x FLAG and mCherry

   Expression

   Mammalian

   Promoter

   human Synapsin

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDNA3-Cerulean-FHA1-NES

   Plasmid

   #138202

   Purpose

   Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   Cerulean-FHA1-NES
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-ATG13

    Plasmid

    #171413

    Purpose

    For the purifcation of human ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ATG13 (ATG13 Synthetic)
    

    Expression

    Mammalian

    Available Since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. MmSphk2

    Plasmid

    #118598

    Purpose

    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice

    Depositor

    Kevin Lynch

    Article

    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

    Insert

    Mus musculus Sphingosine Kinase 2 (Sphk2 Mouse)
    

    Tags

    FLAG

    Expression

    Yeast

    Promoter

    GAL1, 10

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMJB1-RPS22B-Cl

    Plasmid

    #160431

    Purpose

    Expresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoter

    Depositor

    Gary Stormo

    Article

    Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

    Insert

    RPS22B (RPS22B Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Removed introns, shuffled codons to change nucleo…

    Promoter

    GAL1 and S. cerevisiae GAL1

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3.1(-) + SLNCR1

    Plasmid

    #86816

    Purpose

    mammalian expression of lncRNA LINC00673/SLNCR1

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCT

    Plasmid

    #60620

    Purpose

    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB3034(X-3 MarkerFree)

    Plasmid

    #73272

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. POT4-RFP

    Plasmid

    #65320

    Purpose

    receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. NAX_CAGGS_CAS9_m4_KanR neo

    Plasmid

    #167869

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-bArrestin2-TevC-P2A-TdTomato

    Plasmid

    #89869

    Purpose

    expresses iTango bArrestin2-TevC component and TdTomato bicistronically

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    bArrestin2-TevC-P2A-TdTomato
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    R173H in TdTomato (please see depositor comments …

    Promoter

    CMV

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. NAX_CAGGS_CAS9_m4_VPR_Hygro

    Plasmid

    #167883

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. mcherry-p62 Double Mutation/Silent

    Plasmid

    #187982

    Purpose

    K7A/D69A p62 double mutant (PB1AA). Internal reference: SMC532

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D7A, D69A, silent mutation

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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