We narrowed to 14,438 results for: NTS

1. pDECKO_Malat1_B

   Plasmid

   #72621

   Purpose

   Expresses two gRNAs targeting MALAT1 promoter

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward Malat1
   

   Expression

   Mammalian

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQCas12j2

   Plasmid

   #189780

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoter

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Insert

   Cas12j2
   

   Use

   CRISPR; Gateway compatible cas12j2 entry clone

   Tags

   NLS

   Expression

   Plant

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ131C2.0

   Plasmid

   #99886

   Purpose

   Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   OsU6

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ132C2.0

   Plasmid

   #99889

   Purpose

   Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   OsU6

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC

   Plasmid

   #192406

   Purpose

   To be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::Turbo GFP
   

   Use

   Synthetic Biology

   Tags

   N7

   Expression

   Plant

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. mVenus-RnBarr2

   Plasmid

   #137799

   Purpose

   Visualization of beta-arrestin2

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Rat beta-arrestin2 (Arrb2 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL2-100bp

   Plasmid

   #16294

   Depositor

   Wafik El-Deiry

   Article

   Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

   Insert

   p53 promoter fragment (TP53 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   fragment -88 to +12 relative to first transcripti…

   Available Since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. TDP43 NOTAG3

   Plasmid

   #28208

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   aa170-414 only

   Available Since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

9. sgRNA scaffold-spacer-human U6

   Plasmid

   #214697

   Purpose

   provide "sgRNA scaffold-spacer-human U6" fragment

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Insert

   sgRNA scaffold-spacer-human U6
   

   Use

   CRISPR

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pROF394

    Plasmid

    #155333

    Purpose

    Expression of PULSE-controlled LFY-VP16-2A-RLuc

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    (etr)8-(C120)5-PhCMVmin-LFY-VP16-NLS-2A-RLuc-TSV40
    

    Use

    Synthetic Biology; Eukaryotic expression

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS416-Aga1p-FAPB2.3.6L1TAG

    Plasmid

    #158148

    Purpose

    Yeast display reporter for non-display yeast strains (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p-FAPB2.3.6
    

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first position of th…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOD_A0601

    Plasmid

    #91016

    Purpose

    Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9_D10A nickase
    

    Use

    CRISPR

    Mutation

    D10A

    Promoter

    35S

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL2-50bp

    Plasmid

    #16295

    Depositor

    Wafik El-Deiry

    Article

    Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

    Insert

    p53 promoter fragment (TP53 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    fragment -38 to +12 relative to first transcripti…

    Available Since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB528

    Plasmid

    #74870

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGE652

    Plasmid

    #153230

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCTCON2-GFP-TAG

    Plasmid

    #158131

    Purpose

    Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    sfGFP
    

    Expression

    Yeast

    Mutation

    Introduced a stop codon in place of the tyrosine …

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. FusionRed-VASP-5

    Plasmid

    #56136

    Purpose

    Localization: Focal Adhesions, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    VASP
    

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available Since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pBABE-neo largeTcDNA delta434-444

    Plasmid

    #8582

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    deletion in part of the bipartite p53 binding dom…

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

19. mCer-Vamp2(qv/fw)

    Plasmid

    #53238

    Purpose

    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Mutated Q76 to V; F77 to W.

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pJG363

    Plasmid

    #91183

    Purpose

    BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nAtCas9_D10A+gNt_R2+donor
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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