We narrowed to 17,700 results for: SHE

1. WDR5

   Plasmid

   #36900

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   WDR5 (WDR5 Human)
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   mutation version of plasmid #60121 3UR4

   Available Since

   Aug. 8, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pAIO-AsCpf1

   Plasmid

   #137846

   Purpose

   AsCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

   Depositor

   Josh Beck

   Article

   Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

   Insert

   AsCpf1-NLS-FLAG
   

   Use

   CRISPR

   Available Since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7_circRAJ31_C873U

   Plasmid

   #69932

   Purpose

   Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter.

   Depositor

   Alfonso Jaramillo

   Article

   Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

   Insert

   pT7_circRAJ31_C873U
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Hs.KRAS4a Q61L

   Plasmid

   #83171

   Purpose

   Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61L mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   Q61L

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPNT6-eGFP

   Plasmid

   #71303

   Purpose

   This vector can be used for homologous recombination and allows to obtain recombinant neomycin resistant fluorescent cells that can loose fluorescence after Cre expression

   Depositor

   Gian Luca Forni, Marco Musso

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. LMNB2

   Plasmid

   #36896

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   LMNB2 (LMNB2 Human)
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   contains Laminb2 Ig-like domain, amino acid resid…

   Available Since

   June 19, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

7. MAT2B

   Plasmid

   #39080

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MAT2B (MAT2B Human)
   

   Tags

   Tev His Flag

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pLPS-hAkt1-gfp

   Plasmid

   #49388

   Purpose

   mammalian expression of human Akt1 fused to EGFP

   Depositor

   Randall McKinnon

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Cre/Lox; Acceptor vector

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   tga to cca at nt 1725 with respect to GenBank ID …

   Promoter

   CMV

   Available Since

   Nov. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

9. Hs.KRAS4b A146T

   Plasmid

   #83150

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), A146T mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   A146T

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid

    #174136

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Hs.KRAS4a Q61R

    Plasmid

    #83172

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61R mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61R

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. DHRS4

    Plasmid

    #39071

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DHRS4 (DHRS4 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pTXB1-CRP/CAP

    Plasmid

    #51563

    Purpose

    Expression of E. coli CRP/CAP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

    Depositor

    Sankar Adhya, Francisco Malagon

    Insert

    CRP/CAP
    

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. ASPH

    Plasmid

    #39094

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ASPH (ASPH Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1

    Plasmid

    #163319

    Purpose

    Encodes for first COVID protein - NSP1 fused to nano-luciferase

    Depositor

    Dawn Chandler

    Insert

    Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Luciferase

    Tags

    NanoLuc

    Expression

    Mammalian

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Myc-gamma3 (in pCDNA3.1)

    Plasmid

    #118041

    Purpose

    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3 (Gabrg3 Rat)
    

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Myc-gamma1 (in pCDNA3.1)

    Plasmid

    #118038

    Purpose

    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG1 (Gabrg1 Rat)
    

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Myc-gamma3 (in pCAGGS)

    Plasmid

    #118042

    Purpose

    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3 (Gabrg3 Rat)
    

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. POT5-TEF2p-ycrtYB-ADH1t

    Plasmid

    #65359

    Purpose

    TU for combinatorial construction of ?-carotene pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    TEF2p-ycrtYB-ADH1t
    

    Use

    Unspecified

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. POT2-TEF2p-ycrtE-ADH1t

    Plasmid

    #65353

    Purpose

    TU for combinatorial construction of ?-carotene pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    TEF2p-ycrtE-ADH1t
    

    Use

    Unspecified

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEF1-puro/hpb1(short)

    Plasmid

    #59973

    Purpose

    integrin b1 short headpiece expression (beta-I, hybrid, PSI domains) expression

    Depositor

    Timothy Springer

    Insert

    human integrin b1 (short) (ITGB1 Human)
    

    Tags

    Base coiled-coil and His-tag

    Expression

    Mammalian

    Promoter

    EF1a promoter

    Available Since

    Sept. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

22. Hs.KRAS4b G12A

    Plasmid

    #83142

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12A mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12A

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLenti pTERT sgRNA2(MS2) mCherry

    Plasmid

    #171055

    Purpose

    Targeting of Vp64-dCas9 activation system to hTERT promoter

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    pTERT sgRNA2(MS2) mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. ANKRA2

    Plasmid

    #36904

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ANKRA2 (ANKRA2 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    amino acid residues 148-313 (ANK repeats)

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

25. pPB-CAG-cNanog-pgk-hph

    Plasmid

    #74907

    Purpose

    Mammalian expression of cNanog

    Depositor

    Jose Silva

    Article

    Theunissen et al Development. 2011 Nov;138(22):4853-65. d

    Insert

    Nanog
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pMT-DREDD-V5-His

    Plasmid

    #63749

    Purpose

    Expresses drosophila DREDD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    Death related ced-3/Nedd2-like caspase isoform E (Dredd Fly)
    

    Tags

    V5-6XHis

    Expression

    Insect

    Promoter

    pMT

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. MS2_SC2_S_Lambda

    Plasmid

    #175048

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Lambda SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    Lambda (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. POT4-TDH3p-ycrtI-ADH1t

    Plasmid

    #65355

    Purpose

    TU for combinatorial construction of ?-carotene pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    TDH3p-ycrtI-ADH1t
    

    Use

    Unspecified

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. pTRIPZ (M)-HT-Cbx7-ATLm

    Plasmid

    #82522

    Purpose

    Lentivirus vector. Expresses mutant Halotag-Cbx7-ATLm fusion proteins in mammalian cells. Cbx7 ATL mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.

    Depositor

    Xiaojun Ren

    Insert

    Chromobox Homolog 7 (CBX7 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    mutation of RKR70-72, K75, and KR77-78 to AGA70-7…

    Promoter

    CMV

    Available Since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. pEGFP-rbNHERF1

    Plasmid

    #31636

    Depositor

    Edward Weinman

    Article

    Voltz et al J Biol Chem. 2007 Nov 16. 282(46):33879-

    Insert

    solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Feb. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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