We narrowed to 10,783 results for: T7

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SAE1

Plasmid

#175241

Purpose

Bacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimer

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

SAE1 (SAE1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-M2-S2-S3-S4T3D

Plasmid

#33302

Depositor

Terence Dermody

Article

Kobayashi et al Virology. 2010 Mar 15;398(2):194-200. Ep

Inserts

S2 (S2 Orthoreovirus T3D)
S3 (S3 Orthoreovirus T3D)
S4 (S4 Orthoreovirus T3D)
M2 (M2 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pET-NtLNtC60αESC60β

Plasmid

#160875

Purpose

Expression of N. tabacum Rubisco large subunit (rbcL), Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coli

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, groES

Expression

Bacterial

Promoter

rbcL: pBAD, chaperones: T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

Plasmid

#168192

Purpose

HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-LaG6-(EAAAK)4-OGT(4)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTEV5_ RpMatB_2

Plasmid

#78206

Purpose

Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding

Depositor

Martin Webb

Article

Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

Insert

matB (His6 C106A S170A R286C Q457C K488A)

Tags

His6

Expression

Bacterial

Mutation

C106A S170A R286C Q457C K488A

Promoter

T7

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pTU398

Plasmid

#178980

Purpose

13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Insert

gRAMP

Tags

Strep-Tag II SUMO

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_WTDHFR_WTTS

Plasmid

#91226

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GDI2

Plasmid

#67093

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GDI2 (GDI2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pNP561

Plasmid

#222723

Purpose

Target and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Tags

6xHis

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET sfGFP(150TAG)

Plasmid

#133455

Purpose

expresses sfGFP with an unnatural amino acid at the 150th position in bacterial cells

Depositor

Yu-Hsuan Tsai

Article

Williams et al Amino Acids. 2021 Jan;53(1):89-96. doi:

Insert

sfGFP

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ABKAR(T/A)

Plasmid

#140052

Purpose

Negative control mutant for ABKAR biosensor.

Depositor

Jin Zhang

Article

Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

Insert

ABKAR(T/A)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

Nov. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a(+) 6xHis-TEV-IFIT1

Plasmid

#53557

Purpose

bacterial expression of IFIT1

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT1 (IFIT1 Human)

Tags

6xHIS and TEV

Expression

Bacterial

Promoter

T7

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only
pET26_MraYaa

Plasmid

#100166

Purpose

recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Chung et al Science. 2013 Aug 30;341(6149):1012-1016

Insert

mraY (phospho-MurNAc-pentapeptide translocase)

Tags

MBP-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-EF20

Plasmid

#52645

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF20

Expression

Mammalian

Mutation

D362N/D366N mutated from GCaMP3

Promoter

T7

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
pHB-2

Plasmid

#140955

Purpose

For PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.

Depositor

Kang Zhou

Article

Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

Insert

phaCAB

Expression

Bacterial

Mutation

R238S mutation in PhaC

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-BCA-Q11

Plasmid

#185781

Purpose

Encodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

BCA-Q11

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRSETb-ffDronpa

Plasmid

#78182

Purpose

Expresses ffDronpa E. coli strains

Depositor

Peter Dedecker

Article

Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

Insert

ffDronpa

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAcrVIB1

Plasmid

#184566

Purpose

Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

anti-CRISPR protein inhibiting Cas13b nucleases

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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TAL2430

Plasmid

#36829

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HDAC2-TALEN_Left (Hdac2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pPbuCas13b

Plasmid

#184565

Purpose

Inducible expression of PbuCas13b nuclease in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Cas13b nuclease from Prevotella buccae

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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