We narrowed to 11,284 results for: VARS

1. AT1G06840 X062_pECIA2

   Plasmid

   #115052

   Purpose

   Bait vector AT1G06840 X062_pECIA2 should be used with prey vector AT1G06840 X062_pECIA14.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT1G06840 (AT1G06840 Mustard Weed)
   

   Tags

   Fc, V5 and hexahistidine tags

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AT2G19230 M11_pECIA2

   Plasmid

   #114949

   Purpose

   Bait vector AT2G19230 M11_pECIA2 should be used with prey vector AT2G19230 M11_pECIA14.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT2G19230 (AT2G19230 Mustard Weed)
   

   Tags

   Fc, V5 and hexahistidine tags

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AT1G29730 X066_pECIA14

   Plasmid

   #114855

   Purpose

   Prey vector AT1G29730 X066_pECIA14 should be used with bait vector AT1G29730 X066_pECIA2.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT1G29730 (AT1G29730 Mustard Weed)
   

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AT3G02880 J3_pECIA14

   Plasmid

   #114790

   Purpose

   Prey vector AT3G02880 J3_pECIA14 should be used with bait vector AT3G02880 J3_pECIA2.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT3G02880 (AT3G02880 Mustard Weed)
   

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. AT1G51890 M5_pECIA14

   Plasmid

   #114744

   Purpose

   Prey vector AT1G51890 M5_pECIA14 should be used with bait vector AT1G51890 M5_pECIA2.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT1G51890 (AT1G51890 Mustard Weed)
   

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERP2-FOSB

   Plasmid

   #192901

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of FOSB

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   FOSB (FOSB Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184V

   Plasmid

   #106190

   Purpose

   Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-Venus-iGluSnFR.A184V
   

   Use

   AAV

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

   Promoter

   CAG

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221 SARS-CoV-2 S-frag5_nostop

   Plasmid

   #153177

   Purpose

   Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth, Mikko Taipale

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   S-frag5
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode

   Plasmid

   #170254

   Purpose

   Barcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Lentiviral

   Mutation

   A662C, N664C, V667L

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pS6-TGFB2-STREP

    Plasmid

    #128503

    Purpose

    Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t STREP(2x) tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    TGFB2 (TGFB2 Human)
    

    Tags

    STREP (2X) tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223 SARS-CoV2 TEV-N

    Plasmid

    #154424

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    N (N SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    Sept. 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pDONR223_SV2C_WT

    Plasmid

    #81924

    Purpose

    Gateway Donor vector containing SV2C, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SV2C (SV2C Human)
    

    Use

    Gateway entry vector

    Mutation

    F346S

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPGK-T7/2-CD44v3 (-cyt)

    Plasmid

    #137813

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v3 (-cyt) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region, N280D in the V3 region

    Promoter

    PGK

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139095

    Purpose

    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    KRAB-dSpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGBT-RP8.1

    Plasmid

    #189846

    Purpose

    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 1.

    Depositor

    Stephen Ekker

    Article

    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    mRFP and tagBFP

    Promoter

    beta Actin

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ACTB-(A)3x

    Plasmid

    #112057

    Purpose

    ACTB mRNA tagged with 3 copies of Riboglow (variant A)

    Depositor

    Amy Palmer

    Article

    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

    Insert

    ACTB (ACTB Human)
    

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    3 copies of Riboglow tag (variant A) after stop c…

    Promoter

    CMV

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. ACTB-(A)2x

    Plasmid

    #112056

    Purpose

    ACTB mRNA tagged with 2 copies of Riboglow (variant A)

    Depositor

    Amy Palmer

    Article

    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

    Insert

    ACTB (ACTB Human)
    

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    2 copies of Riboglow tag (variant A) after stop c…

    Promoter

    CMV

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEMS1626

    Plasmid

    #29281

    Purpose

    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple155-Intron-LacZ-NLS
    

    Use

    High copy number homologous recombination plasmid…

    Expression

    Mammalian

    Promoter

    MiniPromoter Ple155 from the human PCP2 gene

    Available Since

    June 10, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pDONR223 SARS-CoV2 TEV-ORF3B

    Plasmid

    #154417

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF3B
    

    Use

    Gateway-compatible entry vector, with insert of o…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    July 7, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. AT1G66830 X005_pECIA14

    Plasmid

    #114810

    Purpose

    Prey vector AT1G66830 X005_pECIA14 should be used with bait vector AT1G66830 X005_pECIA2.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT1G66830 (AT1G66830 Mustard Weed)
    

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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