We narrowed to 18,681 results for: tec

1. pCellFree_G03 ISG15

   Plasmid

   #67054

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   ISG15 (ISG15 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pPSP6

   Plasmid

   #48150

   Purpose

   Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TAL2344

   Plasmid

   #36764

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   PTEN-TALEN_Left (Pten Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

4. TAL2345

   Plasmid

   #36765

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   PTEN-TALEN_Right (Pten Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

5. EYFP-Clathrin-19

   Plasmid

   #56584

   Purpose

   Localization: Clathrin Vesicles, Excitation: 513, Emission: 527

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Clathrin (CLTB Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pGWB527

   Plasmid

   #74869

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. boP58-pBabe.puro(notag)

   Plasmid

   #21885

   Depositor

   David Ron

   Article

   Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

   Insert

   P58 (DNAJC3 )
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 8, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMXs-ms-Sox1

   Plasmid

   #50778

   Purpose

   retroviral expression of mouse Sox1

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

   Insert

   Sox1 (Sox1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TAL2362

   Plasmid

   #36775

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   EZH2-TALEN_Left (Ezh2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

10. dTomato_LD 0_CS G_tTA-BFP

    Plasmid

    #58871

    Purpose

    MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDUAL_2_T5

    Plasmid

    #155175

    Purpose

    see pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGWB525

    Plasmid

    #74867

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJL1-EPA-DNNNS-DQNRT

    Plasmid

    #128397

    Purpose

    In vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Exotoxin A
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    242DNNNS246 and 384DQNRT388 engineered glycosylat…

    Promoter

    T7

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCellFree_G03 GATA1

    Plasmid

    #67068

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GATA1 (GATA1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pNAS1b split mCherry 14-3-3β

    Plasmid

    #111880

    Purpose

    Mode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Type

    Empty backbone

    Use

    Tags

    Split mCherry system: 14-3-3β fused to C-terminal…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOCC193

    Plasmid

    #118903

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Use

    Tags

    MBP, cleavable with 3C protease; mGFP and TwinStr…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHCas9-Nours

    Plasmid

    #107733

    Purpose

    Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Human optimized

    Promoter

    TEF1

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TAL2363

    Plasmid

    #36776

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    EZH2-TALEN_Right (Ezh2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCfB3020(gRNA X-2)

    Plasmid

    #73282

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCellFree_G03 TNF

    Plasmid

    #67062

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TNF (TNF Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only