We narrowed to 7,668 results for: CCH

1. pHES873

   Plasmid

   #87945

   Purpose

   Expresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   pGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Human)
   

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB2875

   Plasmid

   #63640

   Purpose

   pCfB2875 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

   Depositor

   Irina Borodina, Jerome Maury

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Yeast

   Promoter

   none

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16

   Plasmid

   #89872

   Purpose

   expresses CB1R-iTango component in mammalian cells

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   CB1R-V2tail-TevN-BLITz1-TetR-VP16
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. BAS287 C terminal E2C hygBR

   Plasmid

   #74082

   Purpose

   S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, hygB resistance.

   Depositor

   Bassem Al-Sady

   Article

   Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

   Insert

   E2Crimson
   

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pMXs_FLAG-ODC1

   Plasmid

   #133256

   Purpose

   Express FLAG-tagged ODC1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   ODC1 (ODC1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in human cells

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-link-GFPIvy-SpHis5

   Plasmid

   #60783

   Purpose

   Yeast C-terminal tagging vector, which creates gene fusions with GFPIvy using SpHis5 selection

   Depositor

   Linda Huang

   Article

   Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

   Insert

   GFPIvy
   

   Use

   Yeast integrating

   Mutation

   S30R Y39N T65C Q69A S72A F99S N105T Y145F M153T V…

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)

   Plasmid

   #122067

   Purpose

   Expressing Dhh1-PA

   Depositor

   Daniel Klionsky

   Article

   Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

   Insert

   Dhh1-PA (DHH1 Budding Yeast)
   

   Tags

   PA

   Expression

   Yeast

   Mutation

   Aspartic acid 195 to alanine; Glutamic acid 196 t…

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. HsSPHK2

   Plasmid

   #118092

   Purpose

   Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

   Depositor

   Kevin Lynch

   Article

   Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

   Insert

   Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   GAL1, 10

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET151-Vps4(101-437)

   Plasmid

   #87735

   Purpose

   Bacterial expression of N-terminally truncated S. cerevisiae Vps4

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100

   Promoter

   T7

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pG4-E6s-luc

    Plasmid

    #46324

    Depositor

    Steven Brown

    Article

    Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

    Insert

    5xGal4
    

    Use

    Luciferase

    Tags

    E boxes and luciferase

    Expression

    Mammalian

    Mutation

    five copies of a multimerized GAL4 (5×GAL4) site …

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pHCas9-Nours

    Plasmid

    #107733

    Purpose

    Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Human optimized

    Promoter

    TEF1

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-FLAG-mTUT4(D1028A)

    Plasmid

    #60220

    Purpose

    Mammalian expression of FLAG-tagged catalytic mutant of TUT4

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

    Insert

    TUT4 (Tut4 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D1028A

    Promoter

    CMV

    Available Since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pGST-TEV-yVms1(1-417,delta(38-69))

    Plasmid

    #128739

    Purpose

    Bacterial overexpression and crystallization

    Depositor

    Chris Hill

    Article

    Nielson et al Mol Cell. 2017 Nov 16;68(4):673-685.e6.

    Insert

    Vms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    residues 1-417 with deletion of 38-69

    Promoter

    T7

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUAST mito roGFP2-Orp1

    Plasmid

    #64997

    Purpose

    for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophila

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-HJURP-CBP

    Plasmid

    #30393

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    HJURP CenpA Binding Domain (CENPA Budding Yeast, Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

16. NAX_CAGGS_CAS9_m4_VPR_EYFP

    Plasmid

    #167882

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pITGFP89

    Plasmid

    #83560

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter -yEGFP-CLN2PEST
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFS485

    Plasmid

    #89348

    Purpose

    Leu1 integrating plasmid with cdc25 genomic fragment

    Depositor

    Nick Rhind

    Article

    Keifenheim et al Curr Biol. 2017 May 22;27(10):1491-1497.

    Insert

    cdc25 (cdc25 Fission Yeast)
    

    Expression

    Yeast

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB2989

    Plasmid

    #63636

    Purpose

    pCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    none

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. IpO

    Plasmid

    #48233

    Purpose

    Base vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

    Inserts

    URA3 3' fragment
    URA3 5' fragment
    

    Use

    Routine cloning vector

    Mutation

    -242 upstream to URA3 ORF +495 and URA3 ORF from …

    Promoter

    URA3 promoter and none

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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