We narrowed to 11,070 results for: aga

Showing: 6061 - 6080 of 11070 results

Results per page:

Sort results by:

His-PHD2-181-426

Plasmid

#223551

Purpose

His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

Depositor

Somshuvra Mukhopadhyay

Article

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-180.

Promoter

trc

Available Since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJEP325-pAAV-FullH1TO-SaCa9sgRNAi(CREB)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

Plasmid

#113702

Purpose

Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB. Followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV, CRISPR, and Mouse Targeting

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTT5-hSDC1-CS1/CS2

Plasmid

#52370

Purpose

expresses human Syndecan-1 point mutant at S184A and S194A in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Article

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Insert

SDC1 (SDC1 Human)

Expression

Mammalian

Mutation

R95Q, S184A and S194A (according to numbering in …

Promoter

CMV

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
pLX304-BCL-XL-Y195F

Plasmid

#203573

Purpose

Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

Depositor

Abhishek Chakraborty

Article

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

Changed Tyrosine 195 to Phenylalanine for partial…

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGLS3-5xHRE-p53(K120R/K164R)

Plasmid

#72556

Purpose

Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R/K164R.

Depositor

Ester Hammond

Article

Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

Insert

p53 (TP53 Human)

Tags

5XHRE

Expression

Mammalian

Mutation

K120R/K164R

Promoter

E1B minimal promoter

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-pegRNA-SNCA-A53T

Plasmid

#181738

Purpose

Transiently expressing a pegRNA to introduce SNCA-A53T mutation in human cells

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for SNCA-A53T (SNCA Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST53-LRRK2-3XKD-R1441C

Plasmid

#25050

Depositor

Mark Cookson

Article

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2-sgCD20

Plasmid

#209749

Purpose

Lentiviral transfer plasmid to express Cas9 and a gRNA targeting the human CD20 gene, MS4A1.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-PCAF (delta5-53a.a.)

Plasmid

#65388

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

PCAF (KAT2B Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

lost 13-159bp of the ORF

Promoter

CMV

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only
Lenti-sgRNA-Cre-GpNLuc-sgmcGAS

Plasmid

#208385

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine cGAS gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgcGAS (Cgas Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJHG003

Plasmid

#126454

Purpose

Expresses Cas9-XTEN-dTdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-XTEN-dTdT (DNTT Streptococcus pyogenes, Human)

Expression

Mammalian

Mutation

Added D343E and D345E to inactivate TdT.

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KIT gRNA (BRDN0001145740)

Plasmid

#77560

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIT

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KIT (KIT Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA216-CoV2-RBD-beta variant

Plasmid

#194598

Purpose

Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

Depositor

Ben Murrell

Article

Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

Insert

CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST53-LRRK2-3XKD-Y1699C

Plasmid

#25051

Depositor

Mark Cookson

Article

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
FUW-CAGGS-ERAS

Plasmid

#52416

Purpose

Lentiviral vector encoding constitutive expression of human ERAS cDNA

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

ERAS (ERAS Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CAGGS

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK8 gRNA (BRDN0001147976)

Plasmid

#78049

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK8 (MAPK8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSico_U6-Pan-PGC1a sgRNA

Plasmid

#165426

Purpose

Expression of gRNA against human Total PGC-1a variants

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

gRNA against human Total PGC-1a variants (PPARGC1A Human, Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-dCTNNA1

Plasmid

#176032

Purpose

A knock-out vector for dog CTNNA1 (alpha-1-catenin)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog CTNNA1 (alpha-1-catenin) gene and the cDNA of Cas9 (CTNNA1 )

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pChuk

Plasmid

#87033

Purpose

express IKKalpha in mammalian cells

Depositor

Georgios Stathopoulos

Article

Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

Insert

IKKalpha (Chuk Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pIS1 INSIG2

Plasmid

#60798

Purpose

Luciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and wild-type miR-155 sites

Depositor

David Bartel

Article

Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

Insert

INSIG2 3'UTR and wild-type miR-155 binding site (INSIG2 Human)

Use

Luciferase

Expression

Mammalian

Promoter

thymidine kinase (HSV-TK promoter)

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge