We narrowed to 8,515 results for: Set

1. AcrIIC1X

   Plasmid

   #128112

   Purpose

   AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9

   Depositor

   Dominik Niopek

   Article

   Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

   Insert

   AcrX (AcrIIC1 N3F/D15Q/A48I)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas9GG

   Plasmid

   #131009

   Purpose

   Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Inserts

   direct repeat of SpCas9
   promoter PJ23119
   mRFP expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_R0040 TetR

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMyc2ElbLuc,

   Plasmid

   #53244

   Purpose

   reporter plasmid containing 2 myc cDNA binding sites

   Depositor

   Roger Davis

   Article

   Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

   Insert

   pMyc2ElbLuc (MYC Human)
   

   Use

   Tags

   Expression

   Mutation

   To prepare plasmids with one, two, three, and fou…

   Promoter

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pMOD_C2519

   Plasmid

   #91088

   Purpose

   Module C, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   OsU3

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. KN302: pMVP/SB/eCFP-DEST

   Plasmid

   #121863

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. KK901: pMVP/SB/Zeo-DEST

   Plasmid

   #121868

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Zeo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330_ATP1A1_G3_Dual_sgRNA

   Plasmid

   #173205

   Purpose

   Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

   Plasmid

   #173214

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
   

   Use

   CRISPR; Prime editing (pe3)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. DCT.VV.Orf76

   Plasmid

   #12764

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jon Yewdell, Jack Bennink

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf76
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

10. DCT.VV.Orf98

    Plasmid

    #12786

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf98
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only