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Showing: 6081 - 6100 of 41828 results

  1. pTS2307-Tier1-2xcHS4-PPGK-NLuc_TB

    Plasmid
    #169550
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    cHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Expression
    Mammalian
    Promoter
    PPGK
    Available Since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169550-plasmid-map-sequence-id-366597

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  13. pAS134

    Plasmid
    #211505
    Purpose
    AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Insert
    Venus
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Expression
    Mammalian
    Available Since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211505-plasmid-map-sequence-id-417792

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  17. pAWPSG-Po-nCas9-BE

    Plasmid
    #195740
    Purpose
    Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Po (phoenol-inducible promoter)
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195740-plasmid-map-sequence-id-386428

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  18. pEG302 22aa SunTag NtDRMcd (noNLS) nog

    Plasmid
    #119554
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    NOS_NLS_GB1_noNLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    119554-plasmid-map-sequence-id-238815

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  20. pAS133

    Plasmid
    #211504
    Purpose
    AAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Insert
    mTagBF2
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Expression
    Mammalian
    Available Since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211504-plasmid-map-sequence-id-417794

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Showing: 6081 - 6100 of 41828 results