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p426*-SaSgH

Plasmid

#136994

Purpose

Helper plasmid for SaCas9 gRNA cloning and expression

Depositor

Huimin Zhao

Article

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Type

Empty backbone

Expression

Yeast

Promoter

SNR52

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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EYFP-hCALML3

Plasmid

#47605

Depositor

Emanuel Strehler

Article

Rogers et al J Biol Chem. 2001 Apr 13;276(15):12182-9

Insert

CALML3 (CALML3 Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pmCherry-U6-RNF103-CHMP3

Plasmid

#140584

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA RNF103-CHMP3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGL1_39

Plasmid

#199046

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSa ORI plus NGS barcode 16

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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EZ-L560

Plasmid

#173028

Purpose

Encodes OptoAMP2 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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luxB:Venus+luxA/pRSETB Duet

Plasmid

#197582

Purpose

BRET lux luciferase with Venus for bacterial expression

Depositor

Takeharu Nagai

Article

Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

Insert

LuxA LuxBVenus

Use

E.coli expression

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLTR-RVG

Plasmid

#17577

Depositor

Jakob Reiser

Article

Mochizuki et al J Virol. 1998 Nov . 72(11):8873-83.

Insert

rabies virus glycoprotein (SAD B19 strain)

Use

Lentiviral

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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p426*-SpSgH

Plasmid

#136993

Purpose

Helper plasmid for SpCas9 gRNA cloning and expression

Depositor

Huimin Zhao

Article

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Type

Empty backbone

Expression

Yeast

Promoter

SNR52

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMUT2-pCadBA-mCherry

Plasmid

#192865

Purpose

pMUT2 based vector for sensing pH in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

pH sensitive promoter

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL852

Plasmid

#214252

Purpose

Expression TadCBEd_V106W in rice cells

Depositor

Yong Zhang

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Type

Empty backbone

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZA_CeTRIC-B1_Del

Plasmid

#87344

Purpose

Inducible expression of CeTRIC-B1 in P.Pichia strains

Depositor

Zhenfeng Liu

Article

Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

Insert

TRIC-B1

Tags

polyhistidine

Expression

Yeast

Mutation

Δ248-297

Promoter

AOX1

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTHSSd_14

Plasmid

#59952

Purpose

Triple split (at 67, 601-SZ)

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

Three-fragment T7* RNA polymerase

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pMLM802

Plasmid

#27203

Depositor

Keith Joung

Article

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pMLM800

Plasmid

#27202

Depositor

Keith Joung

Article

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

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EZ-L175

Plasmid

#173025

Purpose

Encodes OptoAMP1 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGL0_13 [pIP501 ORI]

Plasmid

#198927

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIP501 ORI

Use

Synthetic Biology

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMAH-pnGFP-Ultra

Plasmid

#157924

Purpose

Peroxynitrite sensor pnGFP-Ultra in mammalian cells (use with pMAH-POLY-eRF1(E55D))

Depositor

Huiwang Ai

Article

Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

Insert

pnGFP-Ultra

Expression

Mammalian

Promoter

cmv

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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FRP991_PACT1(-1-520)-LexA-ER-Gal4AD

Plasmid

#58438

Purpose

Expresses LexA-ER-Gal4AD from yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-Gal4AD

Expression

Yeast

Promoter

Act1 promoter (-520;1)

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pGEL851

Plasmid

#214251

Purpose

Expression TadCBEd in rice cells

Depositor

Yong Zhang

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Type

Empty backbone

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL855

Plasmid

#214255

Purpose

Expression Td-CBEmax in rice cells

Depositor

Yong Zhang

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Type

Empty backbone

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge