We narrowed to 12,476 results for: pcdna

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pcDNA-Ran-Q69L-mRFP1-polyA

Plasmid

#104560

Purpose

Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran (Ran Mouse)

Tags

mRFP1

Expression

Mammalian

Mutation

Q69L

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA6-IgK-hFRa(23-234)C11S-th-Fc

Plasmid

#177841

Purpose

Mammalian Expression of Folate Receptor

Depositor

Karsten Melcher

Article

Chen et al Nature. 2013 Aug 22;500(7463):486-9. doi

Insert

Folate receptor alpha (FOLR1 Human)

Expression

Mammalian

Mutation

all 11 C mutated to S

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3/flag-pBirA(1-G78)-FRB

Plasmid

#154447

Purpose

expresses pBirA(1-G78)-FRB/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

flag-pBirA(1-G78)-FRB

Expression

Mammalian

Promoter

T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Venus-Flag (865)

Plasmid

#40998

Depositor

Jonathon Pines

Article

Sedgwick et al EMBO J. 2013 Jan 23;32(2):303-14. doi: 1

Type

Empty backbone

Tags

3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S

Plasmid

#166794

Purpose

Mammalian cell expression vector for Flag-tagged DPP9S

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4-RI1-332-MYC-His6

Plasmid

#185351

Purpose

Mammalian expression of Ribophorin I

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Ribophorin I (RPN1 Human)

Tags

Myc, 6xHis

Expression

Mammalian

Mutation

aa 1-332 only

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-dCas9-p300 Core (Y1467F)

Plasmid

#61362

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter

Depositor

Charles Gersbach

Article

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Insert

S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)

Use

CRISPR and Synthetic Biology

Tags

Flag, HA, and SV40 NLS

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 CMV 3xFG eIF4G1 +MIC

Plasmid

#158776

Purpose

pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1 (with microexon)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-tdMCP-msfGFP(V206K)-GBI

Plasmid

#205163

Purpose

express tdMCP-msfGFP(V206K)-GBI

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

tdMCP-msfGFP(V206K)-GBI

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mouse PKA-RIbeta-mEGFP

Plasmid

#45526

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RI beta tagged by mEGFP

Tags

His

Expression

Mammalian

Available Since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

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pEEF1D-FLAG (pcDNA 3.1+) LG6-1

Plasmid

#37365

Depositor

David Litchfield

Article

Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

Insert

EEF1D (EEF1D Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
(294) pcDNA3.1-myc-OGA(1-400)

Plasmid

#168095

Purpose

Myc tagged truncated human OGA catalytic domain of residues 1-400

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

myc-OGA(1-400)

Tags

myc

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (+) -myc-OGA(GS-544)C181

Plasmid

#193996

Purpose

plasmid encoding myc-tagged OGA(GS-544)C181

Depositor

Christina Woo

Article

Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

Insert

myc-OGA(GS-544)C181

Tags

myc

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-3xFLAG-SRSF1 siRNA-resitant

Plasmid

#218974

Purpose

Vector for expressing siRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGGAGUUUGUACGGAAA)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

SRSF1 cDNA

Expression

Mammalian

Mutation

siRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGG…

Promoter

CMV

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag mTOR S2035T D2357E

Plasmid

#26605

Depositor

Jie Chen

Article

Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

Insert

mTOR (MTOR Human)

Tags

Flag

Expression

Mammalian

Mutation

S2035T D2357E

Available Since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-3myc-6His-EZH2 21A

Plasmid

#42663

Depositor

Mien-Chie Hung

Article

Cha et al Science. 2005 Oct 14;310(5746):306-10.

Insert

EZH2 (EZH2 Human)

Tags

3Myc and 6His

Expression

Mammalian

Mutation

S21A

Available Since

July 17, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA4c/His-Xpress-HA-hBIG1(E793K)

Plasmid

#79433

Purpose

Expresses N-terminally His6, Xpress and HA-tagged BIG1(E793K) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

Insert

BIG1 (ARFGEF1 Human)

Tags

HA, His6, and Xpress

Expression

Mammalian

Mutation

changed Glutamic acid 793 to Lysine

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

Plasmid

#158953

Purpose

Express human RAD51(T309A) mutant in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

RAD51 (RAD51 Human)

Tags

2X FLAG 2X STREP

Expression

Mammalian

Mutation

changed threonine 309 to alanine

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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(189) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4)

Plasmid

#160739

Purpose

Expresses a truncated OGT (4.5 TPRs) fused to a GFP nanobody

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nGFP-(EAAAK)4-OGT(4)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA6A-EGFR ICD (645-1186)

Plasmid

#42667

Depositor

Mien-Chie Hung

Article

Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

Insert

EGFR ICD (645-1186) (EGFR Human)

Tags

6xHis and Myc

Expression

Mammalian

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only