We narrowed to 7,109 results for: 11

1. CD4-MKII-f-hLOV1-TEVcs(ENLYFQ/M)- Gal4

   Plasmid

   #163027

   Purpose

   FLiCRE TF with fast kinetics for HEK293T expression

   Depositor

   Alice Ting

   Article

   Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

   Insert

   CD4-MKII-f-hLOV1-TEVcs-Gal4
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

   Plasmid

   #112157

   Purpose

   Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.

   Depositor

   Ben Giepmans

   Article

   de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261

   Insert

   anti-GFP FLIPPER-body
   

   Use

   Tags

   His and Thrombin cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVenusNfN

   Plasmid

   #128564

   Purpose

   Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

   Depositor

   Oliver Vugrek

   Article

   Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

   Insert

   Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVenusCfC

   Plasmid

   #128567

   Purpose

   Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

   Depositor

   Oliver Vugrek

   Article

   Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

   Insert

   Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pVenusCfN

   Plasmid

   #128566

   Purpose

   Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

   Depositor

   Oliver Vugrek

   Article

   Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

   Insert

   Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX6P1-UBE2S-UBD

   Plasmid

   #66713

   Purpose

   Expresses the UBE2S-UBD fusion protein in E. coli

   Depositor

   David Komander

   Article

   Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

   Inserts

   e2-epf (UBE2S )
   isot (USP5 )
   

   Use

   Tags

   GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pNL(CMV)TK2ALuc2/CMV/WPREdU3

   Plasmid

   #163965

   Purpose

   Co-expression of HSV-1 TK and firefly luciferase in mammalian cells

   Depositor

   Jakob Reiser

   Article

   Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

   Insert

   HSV-1 TK and FLuc
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. ARH1-c003

   Plasmid

   #173093

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   ADPRH (ADPRH Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   E2-L357, codon optimized

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. XE118 CAMKII-T286D-CS2+

   Plasmid

   #16736

   Depositor

   Randall Moon

   Article

   Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

   Insert

   CAMKII-T286D (Camk2a Rat)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   threonine 286 mutated to aspartate

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pQTEV-PSMD11

    Plasmid

    #31333

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ALC1-c005

    Plasmid

    #173092

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    CHD1L (CHD1L Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    L613-K879, codon optimized

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CMV-NGLY1-Puro

    Plasmid

    #181916

    Purpose

    Lentiviral plasmid that directs the expression of NGLY1 in mammalian cells.

    Depositor

    Scott Dixon

    Article

    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    N-glycanase 1 (NGLY1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a(+) 6xHis-3xFlag-IFIT5

    Plasmid

    #53561

    Purpose

    bacterial expression of IFIT5

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT5 (IFIT5 Human)
    

    Use

    Tags

    3xFLAG, 6xHIS, and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

14. MacroH2A.2-c002

    Plasmid

    #173089

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROH2A2 (MACROH2A2 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    K167-K372

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AlkB-D135T

    Plasmid

    #160433

    Purpose

    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr

    Depositor

    Tao Pan

    Article

    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

    Insert

    E. coli AlkB
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 135 to Threonine; deleted t…

    Promoter

    T7

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ARH3-c002

    Plasmid

    #173095

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    ADPRS (ADPRS Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    A14-S363

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-4T-1 3xFlag-ERK2

    Plasmid

    #47573

    Purpose

    Bacterial expression plasmid for GST-3xFlag-tagged ERK2

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    3xFlag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

18. ZNF668-Flag

    Plasmid

    #42636

    Depositor

    Shiaw-Yih Lin

    Article

    Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

    Insert

    ZNF668 (ZNF668 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

19. Pb459-mU6-sgRNA-EF1a-PuroR

    Plasmid

    #195507

    Purpose

    piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    Puromycin
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBluescript GPX-1

    Plasmid

    #13038

    Depositor

    Peter Newburger

    Article

    Shen et al J Biol Chem. 1993 May 25. 268(15):11463-

    Insert

    GPX-1 (GPX1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Additional GCG upstream of codon 11. Codon 92 is …

    Promoter

    Available since

    Nov. 7, 2006

    Availability

    Academic Institutions and Nonprofits only