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Addgene

We narrowed to 10,047 results for: yeast

Showing: 6101 - 6120 of 10047 results
  1. pTP-PCR C3

    Plasmid
    #189620
    Purpose
    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Insert
    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Removed repeat region, 10-kbp deletion, and minor…
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. BB3cH_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104907
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pCRISPEY-GAL-Kan-ADE2

    Plasmid
    #232105
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pV1524

    Plasmid
    #111431
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)
    Depositor
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pG2J

    Plasmid
    #102882
    Purpose
    Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.
    Depositor
    Inserts
    OsTIR1
    mGFP-AID
    Use
    Tags
    AID(71-114)
    Expression
    Yeast
    Mutation
    Promoter
    ADH1 and TEF1
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. p403 – TDH3-MTS-mCherry-GFP1-10-His

    Plasmid
    #177730
    Purpose
    mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker
    Depositor
    Insert
    mCherry-GFP1-10
    Use
    Tags
    Mitochondrial Targeting Sequence
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. p415 TEF roGFP2‐Tsa2ΔCPΔCR

    Plasmid
    #83239
    Purpose
    The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.
    Depositor
    Insert
    roGFP2-Tsa2dCpdCr
    Use
    Tags
    roGFP2 fusion with Tsa2dCpdCr
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
  9. pCRISPEY-GAL-Nat-ADE2

    Plasmid
    #232103
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 6101 - 6120 of 10047 results