We narrowed to 10,337 results for: plasmids 101

1. PP_072_pAAV_hSyn_DIO-LR-Voltron2-P2A-LR-CheRiff-HA

   Plasmid

   #230988

   Purpose

   Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

   Depositor

   Adam Cohen

   Article

   Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

   Insert

   Cre-dependent, membrane-localized optopatch for neuronal dendrites
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. psPax2-D64V-NC-mNeon

   Plasmid

   #196509

   Purpose

   mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   mNeon
   

   Use

   Tags

   mNeon

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. plRL2

   Plasmid

   #163631

   Purpose

   Optimized M. tuberculosis CRISPRi plasmid; L5 integrating.

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet-On

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. plRL117

   Plasmid

   #163635

   Purpose

   Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet-On

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZac2.1 gfaABC1D-cyto-GCaMP6f

   Plasmid

   #52925

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Baljit Khakh

   Article

   Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1

   Has Service

   AAV5

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. HDM-Hgpm2

   Plasmid

   #204152

   Purpose

   Lentivirus helper plasmid coding for Gag/Pol

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   HIV-1 gag/pol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. HDM-tat1b

   Plasmid

   #204154

   Purpose

   Lentivirus helper plasmid coding for Tat

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   tat
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBluescriptSKII+ U6-sgRNA(F+E) empty

   Plasmid

   #74707

   Purpose

   Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

   Depositor

   Eugene Yeo

   Article

   Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

   Insert

   U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-DIO-EGFP-WPRE

   Plasmid

   #187103

   Purpose

   Double floxed EGFP under the control of EF1a promoter

   Depositor

   Yongling Zhu

   Article

   Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hsyn-GRAB_HA1h

    Plasmid

    #190473

    Purpose

    Express the high-affinity histamine sensor GRAB_HA1h in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

    Insert

    Histamine sensor GRAB_HA1h (high affinity)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-PGK-ABI-dCas9-P2A-mcherry

    Plasmid

    #121513

    Purpose

    expression of AB1-fused-dcas9 plasmids

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    AB1 and P2A-mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hsyn-GRAB_HA1m

    Plasmid

    #190474

    Purpose

    Express the medium-affinity histamine sensor GRAB_HA1m in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

    Insert

    Histamine sensor GRAB_HA1m (medium affinity)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pP2A_NLS_sfGFP

    Plasmid

    #63709

    Purpose

    Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

    Depositor

    Stanley Qi

    Article

    Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

    Insert

    Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
    

    Use

    Just a donor dna with nanog homologous arm for kn…

    Tags

    sfGFP

    Expression

    Mutation

    Promoter

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJZC588

    Plasmid

    #62315

    Purpose

    sgRNA with 2x MS2 (wt+f6) for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 2x MS2 (wt+f6) binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJZC518

    Plasmid

    #62277

    Purpose

    Yeast dCas9 expression plasmid

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Has mutations in the RuvC1 and HNH domains to ren…

    Promoter

    pTdh3

    Available Since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPR_DMS3g24

    Plasmid

    #89244

    Purpose

    Plasmid encodes a synthetic CRISPR containing 7 copies of a single spacer, which targets a region (gene 24) in the genome of bacteriophage DMS3

    Depositor

    Blake Wiedenheft

    Article

    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

    Insert

    CRISPR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGTPA-lb

    Plasmid

    #171528

    Purpose

    Plasmid contains the Cas12k gRNA for mutant library construction

    Depositor

    Quanjiang Ji

    Article

    Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

    Insert

    Cas12k gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Constitutive promoter J23119

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGTPA

    Plasmid

    #171525

    Purpose

    Plasmid contains the Cas12k gRNA and transposon donor cassette

    Depositor

    Quanjiang Ji

    Article

    Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

    Insert

    Cas12k gRNA, transposon donor cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Constitutive promoter J23119

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-L7-6-DD-T6B-WT-YFP

    Plasmid

    #235145

    Purpose

    Expresses the inducible T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoter

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    DHFR-fused T6B peptide
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    L7-6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-L7-6-FLAG-HA-T6B-WT-YFP

    Plasmid

    #235141

    Purpose

    Expresses the T6B peptide fused with YFP under the Purkinje cell-specific L7-6 promoter

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    T6B peptide
    

    Use

    AAV

    Tags

    FLAG/HA

    Expression

    Mutation

    Promoter

    L7-6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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