We narrowed to 51,173 results for: son

1. pEJS2703-CMV-iNme2Smu-ABE8e-nt

   Plasmid

   #228724

   Purpose

   Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   N-terminal ABE8e with iNme2Smu nCas9 (D16A)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS2711-CMV-Nme2-ABE8e-i1-L10-D56R

   Plasmid

   #228732

   Purpose

   Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2 nCas9 (D16A)-i1-L10-D56R
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A D56R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10

   Plasmid

   #228735

   Purpose

   Expresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid iNme2Smu nCas9 (D16A)-i1-L10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R

   Plasmid

   #228731

   Purpose

   Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2 nCas9 (D16A)-i1-L10-E932R
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEJS2713-CMV-iNme2-ABE8e-i1-L10

   Plasmid

   #228734

   Purpose

   Expresses domain inlaid iNme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid iNme2 nCas9 (D16A)-i1-L10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS2424_Nme2.Smu-ABE8e-i8

   Plasmid

   #201522

   Purpose

   Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i8

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2.Smu-ABE8e-i8
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWR76

   Plasmid

   #202776

   Purpose

   episomal fluorescent reporter plasmid for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   TDH3 promoter (S. stipitis)
   coCherry
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   

   Expression

   Yeast

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p0521s

   Plasmid

   #62862

   Purpose

   Episome vector for Phaeodactylum tricornutum (pCC1BAC-based)

   Depositor

   Hamilton Smith

   Article

   Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Episomal vector for phaeodacty…

   Available Since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDOC-GG

   Plasmid

   #149377

   Purpose

   Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

   Depositor

   Mark Pallen

   Article

   Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

   Inserts

   Fragment containing a kanamycin resistance cassette
   Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
   Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
   Fragment containing an I-SceI recognition sequence
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPtPuc3

    Plasmid

    #62863

    Purpose

    Episome vector for Phaeodactylum tricornutum (pUC-based)

    Depositor

    Hamilton Smith

    Article

    Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Episomal vector for phaeodacty…

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX Rin1-SH2

    Plasmid

    #46474

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Rin1 (RIN1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 54-204

    Promoter

    Tac

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEmerald-LaminB1-10

    Plasmid

    #54140

    Purpose

    Localization: Nuclear Envelope, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. mTagRFP-T-Mito-7

    Plasmid

    #58023

    Purpose

    Localization: Mitochondria, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

    Insert

    Mito (COX8A Human)
    

    Tags

    mTagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. mTagBFP2-ER-5

    Plasmid

    #55294

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    ER (CALR Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pTol1:uas-myc-act-map2k2b-2a-tdTomato

    Plasmid

    #104721

    Purpose

    UAS-driven expression of activated MEK

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    activated map2k2b (map2k2b Zebrafish)
    

    Use

    Zebrafish transposon

    Tags

    2A-tdTomato and myc tag

    Mutation

    serines 221 and 225 to aspartic acid

    Promoter

    10xUAS

    Available Since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-TAG-ERN

    Plasmid

    #80476

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and neomycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Expression

    Mammalian

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. mTagBFP2-TFR-20

    Plasmid

    #55327

    Purpose

    Localization: Transferin Receptor, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    TFR (TFRC Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pEJS614_pTetR-P2A-BFPnls/sgNS

    Plasmid

    #108650

    Purpose

    Non-specific Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Non-specific sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. mRuby2-ZO1-C-14

    Plasmid

    #55916

    Purpose

    Localization: Tight Junctions, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    ZO1 (TJP1 Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP

    Plasmid

    #169332

    Purpose

    Nuclear expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag9 (H2BC21 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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