We narrowed to 58,550 results for: tra

1. pCJR01

   Plasmid

   #187888

   Purpose

   Escherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.

   Depositor

   Gaylen Uhlich

   Article

   Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

   Insert

   DNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
   

   Expression

   Bacterial

   Promoter

   none

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSPPac-hp

   Plasmid

   #48123

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

   Insert

   signal peptide of Pac
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. Zebrabow-GateDest

   Plasmid

   #118221

   Purpose

   For cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.

   Depositor

   Albert Pan, Alex Schier

   Article

   Pan et al Development. 2013 Jul;140(13):2835-46. d

   Insert

   Brainbow 1.0 L - ccdB survival cassette
   

   Use

   Zebrafish expression

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-Flavidin

   Plasmid

   #89881

   Purpose

   Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling

   Depositor

   Mark Howarth

   Article

   Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

   Insert

   Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
   

   Tags

   6 glutamate tag (C terminal on insert)

   Expression

   Bacterial

   Mutation

   K80R, K121R, K132R, K134R

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-TPD54

   Plasmid

   #172448

   Purpose

   Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP tag

   Depositor

   Stephen Royle

   Article

   Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

   Insert

   TPD54 (TPD52L2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe-miR-133

   Plasmid

   #172392

   Purpose

   Retroviral expression vector for direct cardiac reprogramming

   Depositor

   Li Qian

   Article

   Zhou et al Cell Stem Cell. 2019 Jul 3;25(1):149-164

   Insert

   miR-133
   

   Use

   Retroviral

   Available Since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-CD9-(TEV)-tTA

   Plasmid

   #162596

   Purpose

   human CD9-TEV cleavage site-tTA

   Depositor

   Masaharu Somiya

   Article

   Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

   Insert

   human CD9 (CD9 Human)
   

   Tags

   TEV protease cleavage site and tTA

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. gRNA NIA Null

   Plasmid

   #198728

   Purpose

   Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AtNIA1 gRNAs
   

   Expression

   Plant

   Promoter

   pU6-26, pU6-29

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LC001: pMVP/PB/Hygro-DEST

   Plasmid

   #121876

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

    Plasmid

    #159808

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. dTomato_LD 0_CS M_tTA-BFP

    Plasmid

    #58875

    Purpose

    MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGI3EM17

    Plasmid

    #210569

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
    

    Use

    Luciferase

    Tags

    NanoLuc luciferase

    Expression

    Bacterial and Yeast

    Promoter

    SpH2Bpro

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-hSyn-Axon-jGCaMP8f

    Plasmid

    #192615

    Purpose

    AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8f.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    Axon-jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.hSynapsin.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209664

    Purpose

    Expression of iATPSnFR2, medium affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95K.HaloTag
    

    Use

    AAV

    Mutation

    A95K

    Promoter

    hSyn

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TAK11

    Plasmid

    #227095

    Purpose

    Luciferase reporter vector with HIV-1 TAR region cloned in pGL3 Basic vector

    Depositor

    Farah Mustafa

    Article

    Khader et al Commun Biol. 2024 Nov 16;7(1):1521. doi:

    Insert

    CMV Promoter and HIV-1 TAR region
    

    Use

    Luciferase

    Promoter

    CMV

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-TPD54

    Plasmid

    #172449

    Purpose

    Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry tag

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

    Insert

    TPD54 (TPD52L2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-rab9 DN

    Plasmid

    #12664

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB9 (Homo sapiens) (RAB9A Human)
    

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    changed serine 21 to asparagine

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIG-VP16 RARa [TJ#389]

    Plasmid

    #16287

    Depositor

    Thomas Jessell

    Article

    Novitch et al Neuron. 2003 Sep 25. 40(1):81-95.

    Insert

    RAR alpha (RARA Human)
    

    Tags

    VP16

    Expression

    Mammalian

    Available Since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

19. Cav1-mRFP

    Plasmid

    #14434

    Depositor

    Ari Helenius

    Article

    Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

    Insert

    Caveolin-1 (CAV1 Canis familalis)
    

    Tags

    mRFP

    Expression

    Mammalian

    Available Since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)

    Plasmid

    #193049

    Purpose

    Encodes G-418 resistance

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    prsp-27::NeoR::unc-54 3’ UTR
    

    Expression

    Worm

    Promoter

    prsp-27

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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