We narrowed to 17,014 results for: EMB

1. pMaCTag-18

   Plasmid

   #119997

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with mKeima8.5

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mKeima8.5
   

   Use

   Pcr template

   Tags

   mKeima8.5

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-ERas-IH

   Plasmid

   #15688

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

   Insert

   ERas (Eras Mouse)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pMXs-EGFP-Rheb SSVM-IP

   Plasmid

   #13832

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

   Insert

   Rheb SSVM (Rheb Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C181 changed to serine

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pFUS_B8

   Plasmid

   #31025

   Depositor

   Adam Bogdanove, Daniel Voytas

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   LacZ + BsaI restriction sites
   

   Available Since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUS_B9

   Plasmid

   #31026

   Depositor

   Adam Bogdanove, Daniel Voytas

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   LacZ + BsaI restriction sites
   

   Available Since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSG75-β2AR-T4L-sfGFP

   Plasmid

   #102461

   Purpose

   Expresses a fusion protein of β2 adrenergic receptor/T4 lysozyme chimera and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.

   Depositor

   Richard Murray

   Article

   Guo et al bioRxiv 104455

   Insert

   β2AR
   

   Tags

   His tag and sfGFP

   Expression

   Bacterial

   Promoter

   OR2-OR1-Pr

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. SNAP-EF(N)-30nmSAH-GFP(1-10)

   Plasmid

   #61662

   Purpose

   30nm SAH scaffold with EF Hand and split GFP attachment sites

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   SNAP-EF(N)-30nmSAH-GFP(1-10)
   

   Expression

   Mammalian

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cry2PHR - IBAR

   Plasmid

   #176602

   Purpose

   Cry2PHR with C-terminal IBAR domain

   Depositor

   Robert Hughes

   Article

   Wurz et al J Biol Chem. 2022 Aug 18;298(10):102388.

   Insert

   Cry2PHR-mCherry-IBAR
   

   Expression

   Mammalian

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMaCTag-01

   Plasmid

   #119980

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with moxBFP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   moxBFP
   

   Use

   Pcr template

   Tags

   moxBFP

   Promoter

   None

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS426-GFP-Num1-PH

    Plasmid

    #71374

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Num1-PH
    

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain; aa2563-2692

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

11. GGE021

    Plasmid

    #120778

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position2-3. When using the GG platform for expressing only two gene (T2-3_Lip2)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    T2-3_Lip2
    

    Use

    Cloning/assembly plasmid

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACEBac1-NEDD1

    Plasmid

    #178073

    Purpose

    Insect cell expression of NEDD1

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    NEDD1 (NEDD1 Human)
    

    Expression

    Insect

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUS_B7

    Plasmid

    #31024

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TOPO Venus

    Plasmid

    #68493

    Purpose

    TOPO construct expressing Venus for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    split Venus
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. C453-E23: attB4-TRE3Gp>-attB5

    Plasmid

    #162979

    Purpose

    Gateway attB4/attB5 entry clone for inducible mammalian expression using the TRE3G promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Tet-regulated TRE3G minimal promoter
    

    Use

    Synthetic Biology

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDD398

    Plasmid

    #91832

    Purpose

    AID NT-tag linker donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Auxin-inducible degron (AID)
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSP64-H2B-GFP

    Plasmid

    #135605

    Purpose

    Expression of H2B-GFP mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

    Depositor

    Mamiko Yajima

    Article

    Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

    Insert

    H2B-GFP
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    GFP

    Promoter

    Unknown

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac GST-STIL

    Plasmid

    #80265

    Purpose

    baculovirus creation plasmid for GST-STIL

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Tags

    GST

    Expression

    Insect

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. TAL2452

    Plasmid

    #36841

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    RUNX1-TALEN_Left (Runx1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

20. EZ-L498

    Plasmid

    #131771

    Purpose

    PPGK1_FUSN_Citrine _PixE_TCYC1

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PPGK1_FUSN_Citrine _PixE_TCYC1
    

    Use

    Integration into his3 locus

    Expression

    Yeast

    Promoter

    PGK

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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