We narrowed to 15,601 results for: LEA
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pMGF172
Plasmid#96998Purposeish1 mCherry insertion cassette with ura4+ geneDepositorInsertish1 (ish1 Fission Yeast)
UseTagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + ura4(…PromoterN/AAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(WT)
Plasmid#86576PurposeExpresses cleavable human full-length Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130PromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-AVITEV-GFP
Plasmid#74052Purposeretroviral expression plasmid for GFP with N-terminal BirA-biotinylation signal and TEV celavage siteDepositorInsertAVITEV-GFP
UseRetroviralTagsAVI-TEV-tag N-terminal to the GFPExpressionMammalianMutationPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LINXa4-Bre1
Plasmid#73617PurposeControl of the E3 ligase Bre1 with LINXa in yeast.DepositorInsertLINXa4-Bre1dNLS
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11APromoterADH1Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase delta 55
Plasmid#59824PurposeAllows the integration of myc Separase delta 55 in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationdelta 55Promoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-Cb2
Plasmid#53529PurposeExpresses an insect mitochondrial arginyl-tRNA synthetase in E.coliDepositorInsertmitochondrial arginyl-tRNA synthetase
UseTagsHis Tag and ThioredoxinExpressionBacterialMutationchanged Ser4 to Ala4 to permit NcoI cloningPromoterT7Available sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
XE224 human flag-Chibby-pCS3+FLT
Plasmid#16824DepositorInsertflag-Chibby (CBY1 Human)
UseXenopus expressionTagsflagExpressionMutationPromoterAvailable sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-196a-2SM-PGK-GFP-Blast
Plasmid#31512DepositorInsertmiR-196a2 (Mir196a-2 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationseed mutant controlPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
HOXB6-FLAGA-pSP65
Plasmid#20994DepositorInsertHOXB6 (HOXB6 Human)
UseIn vitro trans/transTagsFLAGExpressionMutationPromoterAvailable sinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-rB90
Plasmid#18959DepositorInsertBrevican C-terminus (Bcan Rat)
UseTagsExpressionMammalianMutationRat brevican C-terminus: Signal peptide aa 1-22 …PromoterAvailable sinceDec. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDD2000
Plasmid#101197PurposeName: pBluescript-LANApi,K14. Bidirectional reporter driving LANApi side [green luciferase isoform from pCBG68] and K14 side [red luciferase isoform from pCBR] in pBluescript backbone.DepositorInsertLANApi side [green luciferase isoform of pCBG68], K14 side [red luciferase isoform of pCBR]
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2alpha D291N mutant
Plasmid#128813PurposeTo make virus expressing Doc2alpha D291 mutantDepositorInsertDoc2alpha (Doc2a Rat)
UseLentiviralTagsIRES-EGFPExpressionMutationD291NPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2beta D303N mutant
Plasmid#128815PurposeTo make virus expressing Doc2beta D303N mutantDepositorInsertDoc2beta (Doc2b Rat)
UseLentiviralTagsIRES-EGFPExpressionMutationD303NPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2alpha Halotag
Plasmid#128817PurposeTo make virus expressing Doc2alpha HalotagDepositorInsertDoc2alpha (Doc2a Rat)
UseLentiviralTagsHaloTag-IRES-EGFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2beta Halotag
Plasmid#128818PurposeTo make virus expressing Doc2beta HalotagDepositorInsertDoc2beta (Doc2b Rat)
UseLentiviralTagsHaloTag-IRES-EGFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub(T/A)-RLuc8N-NES
Plasmid#138212PurposeEncodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertPKAsub(T/A)-RLuc8N-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLVX-XBP1 mNeonGreen NLS
Plasmid#115968PurposeER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreenDepositorInsertX-box binding protein 1 (XBP1 Human, Synthetic)
UseLentiviralTagsHA tag, c-myc NLS, and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (Sce)
Plasmid#45576PurposeCodes for the SSA-TLR with the target site for I-Sce I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with I-Sce I TS
+3 mCherry
3' iRFP arm
UseLentiviralTagsExpressionBacterial and MammalianMutationembedded I-Sce I TS from 163-185bp, truncated 25 …PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only
