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evoBxb1_IVT

Plasmid

#222341

Purpose

Plasmid for evolved Bxb1 mRNA in vitro transcription template

Depositor

David Liu

Publication

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved Bxb1

Use

Template for in vitro transcription

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z23

Plasmid

#120066

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Halo

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Halo

Use

Pcr template

Tags

Halo

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Flp-DOG

Plasmid

#80084

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

Depositor

Connie Cepko

Publication

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

Flp-DOG

Expression

Mammalian

Promoter

CAG

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pErbB2-ECFP

Plasmid

#66945

Purpose

Mammalian expression of rat ErbB2 tagged with ECFP

Depositor

Mads Lerdrup, Bo van_Deurs

Publication

Lerdrup et al J Cell Sci. 2006 Jan 1;119(Pt 1):85-95.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ErbB2 (Erbb2 Rat)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pDONR223-PRKAR1B

Plasmid

#23376

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAR1B (PRKAR1B Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
RCAS(A) mPea3 FL (CT#1119)

Plasmid

#13908

Depositor

Cliff Tabin

Publication

Brent et al Development. 2004 Aug . 131(16):3885-96.

Insert

Pea3 (Etv4 Mouse)

Use

Retroviral; Avian expression

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
ZFP36L2

Plasmid

#156149

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ZFP36L2 (ZFP36L2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223 SARS-CoV-2 ORF10wu

Plasmid

#149332

Purpose

Gateway-compatible Entry vector, with insert of ORF10wu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF10wu (ORF10 SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 ORF6_nostop

Plasmid

#149323

Purpose

Gateway-compatible Entry vector, with insert of ORF6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF6 (ORF6 SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 NSP2_nostop

Plasmid

#149305

Purpose

Gateway-compatible Entry vector, with insert of NSP2 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP2 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSMART-Mev1

Plasmid

#65815

Purpose

NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Inserts

mvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
mvaS: hydroxymethylglutaryl-CoA synthase

Expression

Bacterial

Promoter

insulated mipAp from E. coli and insulated waaHp…

Available since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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F2-bio-His

Plasmid

#52179

Purpose

Expresses full-length Prothrombin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Publication

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

F2 (F2 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1mLCAD_FLAG

Plasmid

#24484

Depositor

Eric Verdin

Publication

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Tags

FLAG

Expression

Mammalian

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
GFP Snail 4SA

Plasmid

#16227

Depositor

Mien-Chie Hung

Publication

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

4SA: S108A, S112A, S116A, S120A

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-PSDΔ1,2-FRBx3

Plasmid

#225827

Purpose

A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

Depositor

Haruo Kasai

Publication

Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

Insert

PSDΔ1,2-FRBx3

Use

AAV

Expression

Mammalian

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pSH824-ssr

Plasmid

#198027

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Publication

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Apr resistance cassette

Expression

Bacterial

Promoter

AprR promoter

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-CCSP:GFP

Plasmid

#68487

Purpose

Retrovirus expressing GFP driven by CCSP promoter

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

CCSP and LTR

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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MXS_BiTet

Plasmid

#62423

Purpose

MXS_chaining vector with BiTet

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Bidirectional tetracycline inducible promoter

Use

Synthetic Biology

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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M-tdTom-ST1

Plasmid

#48678

Purpose

Mammalian tdTomato activation reporter for ST1 with GTCCCCTCCACCCCACAGTG protospacer

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, ST1-PAM, and tdTomato reporter. Compatible with S. thermophilus #1 Cas9

Use

CRISPR

Promoter

Sp6

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGEX-6P-Cdh1wt (570)

Plasmid

#39877

Depositor

Jonathon Pines

Insert

FZR1 (FZR1 Human)

Tags

GST

Expression

Bacterial

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles