We narrowed to 17,014 results for: EMB

1. pMaCTag-09

   Plasmid

   #119988

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with mVenus

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mVenus
   

   Use

   Pcr template

   Tags

   mVenus

   Promoter

   None

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMTB-Multibow-mR

   Plasmid

   #60990

   Purpose

   Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound dTomato

   Depositor

   Sean Megason

   Article

   Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

   Insert

   dTomato
   

   Use

   Zebrafish

   Tags

   membrane tag

   Promoter

   Bactin2

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pOGG031

   Plasmid

   #113994

   Purpose

   Level 0 module. IPTG promoter for inducible expression

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   IPTG-inducible promoter
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOGG016

   Plasmid

   #113990

   Purpose

   Vector construction module. Endlinker/terminator module to circularize plasmid following Part 6

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Endlinker/terminator Part 6
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMMBAD16

   Plasmid

   #20176

   Depositor

   Hideo Iwai

   Article

   Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

   Insert

   src homology domain 3 (CRK Human)
   

   Tags

   NpuDnaEc16

   Expression

   Bacterial

   Available Since

   Aug. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHJ386

   Plasmid

   #20679

   Depositor

   Stephen Shaw

   Article

   Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

   Insert

   Moesin (MSN Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Lys 253, 254, 262, 263 to Asn; Changed Th…

   Available Since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pc3XB-ZF70

   Plasmid

   #13193

   Depositor

   Daniel Voytas

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Insert

   ZF70
   

   Expression

   Mammalian

   Available Since

   Oct. 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMaCTag-29

   Plasmid

   #120008

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with Strep-II

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Strep-II
   

   Use

   Pcr template

   Tags

   Strep-II

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMB-TLEr

   Plasmid

   #61188

   Purpose

   Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   MtRAB7A2
   

   Tags

   mCherry

   Expression

   Plant

   Promoter

   MtBCP1

   Available Since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2-AcrIIA4

    Plasmid

    #174034

    Purpose

    In vitro transcription of anti-CRISPR AcrIIA4 mRNA

    Depositor

    James Gagnon

    Article

    Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

    Insert

    AcrIIA4
    

    Use

    In vitro transcription

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2-AcrIIA2

    Plasmid

    #174033

    Purpose

    In vitro transcription of anti-CRISPR AcrIIA2 mRNA

    Depositor

    James Gagnon

    Article

    Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

    Insert

    AcrIIA2
    

    Use

    In vitro transcription

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWZ414-F55

    Plasmid

    #23079

    Depositor

    Sharon Dent

    Article

    Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

    Insert

    yeast Histone H3-2 and Histone H4-2
    

    Expression

    Yeast

    Mutation

    Histone H3 changed Lysine 9 to Arginine, H3 K9R …

    Available Since

    March 3, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pFA1

    Plasmid

    #46789

    Purpose

    To express Fun30 in trans in budding yeast

    Depositor

    Patrick Varga-Weisz

    Article

    Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

    Insert

    FUN30 (FUN30 Budding Yeast)
    

    Tags

    V5

    Expression

    Yeast

    Promoter

    PGAL1

    Available Since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pFA6a-kanMX6-P41nmt1-mYFP

    Plasmid

    #105141

    Purpose

    Yeast gene targeting

    Depositor

    Thomas Pollard, Jian-Qiu Wu

    Article

    Wu et al Dev Cell. 2003 Nov;5(5):723-34.

    Insert

    kanMX6-P41nmt1-mYFP
    

    Tags

    41nmt1-mYFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    41nmt1

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcryBFP_Umyo18bGFP

    Plasmid

    #172527

    Purpose

    alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish Myo18b.

    Depositor

    Joachim Berger

    Article

    Berger et al Hum Mol Genet. 2017 Mar 15;26(6):1146-11

    Insert

    myo18b
    

    Expression

    Mammalian

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Venus-7aa-MAO-pEGFP-C3

    Plasmid

    #166765

    Purpose

    To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.

    Depositor

    David Andrews

    Insert

    MAO tail anchor (MAOA Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCherry-C1-DH

    Plasmid

    #67898

    Purpose

    Expresses a fusion of mCherry with the DH domain (155-347) of p63RhoGEF in mammalian cells

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    mCherry-DH
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTAL4

    Plasmid

    #31035

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    TALEN-FokI nuclease backbone
    

    Tags

    AcV5 and FokI nuclease

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. TAL2453

    Plasmid

    #36842

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    RUNX1-TALEN_Right (Runx1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

20. C512-E04: attB5r-mCherry-nostop-attB1r

    Plasmid

    #162941

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal mCherry fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    monomeric Cherry fluorescent protein
    

    Use

    Synthetic Biology

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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