We narrowed to 16,915 results for: EMB

1. M-R742X (BF7-2)

   Plasmid

   #21372

   Depositor

   Gregory Germino

   Article

   Hanaoka et al Nature. . 408(6815):990-4.

   Insert

   autosomal dominant polycystic kidney disease type II (PKD2 Human)
   

   Use

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   changed R742 to stop codon

   Promoter

   Available since

   Feb. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

2. YIplac211-VPS16-iGFPx6

   Plasmid

   #105275

   Purpose

   Plasmid for pop-in/pop-out replacement of Vps16 with Vps16-iGFPx6.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Vps16 portion (VPS16 Budding Yeast)
   

   Use

   Tags

   iGFPx6

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDTnLAPP2A frame 0

   Plasmid

   #28226

   Depositor

   Harald von Melchner

   Article

   Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

   Insert

   Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
   

   Use

   Retroviral

   Tags

   EGFP and nLAP-tag

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pXL-BacII-CAG-Venus

   Plasmid

   #170518

   Purpose

   Plasmid containing piggyBac inverted terminal repeats (ITRs) sequences flanking CAG and Venus

   Depositor

   Joshua Sanes

   Article

   Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

   Insert

   CAG-Venus
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. C511-E02: attB5r-NanoLuc-nostop-attB1r

   Plasmid

   #162938

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Nanoluciferase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. C413-E33: attB4-UbCp>-attB1r

   Plasmid

   #162925

   Purpose

   Gateway attB4/attB1r entry clone for mammalian expression using the human Ubiquitin C promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   human Ubiquitin C promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C232-E29: attB2r-mClover3-attB3

   Plasmid

   #162906

   Purpose

   Gateway attB2r/attB3 entry clone for construction of C-terminal mClover3 fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   monomeric Clover3 fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. C232-E30: attB2r-mRuby3-attB3

   Plasmid

   #162907

   Purpose

   Gateway attB2r/attB3 entry clone for construction of C-terminal mRuby3 fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   monomeric Ruby3 fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. C234-E25: attB2r-SNAP-attB3

   Plasmid

   #162914

   Purpose

   Gateway attB2r/attB3 entry clone for construction of C-terminal SNAP tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   SNAP tag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TAL2218

    Plasmid

    #36677

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    MUTYH-TALEN_Left (Mutyh Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pDD397

    Plasmid

    #91822

    Purpose

    AID FP-slot donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Auxin-inducible degron (AID)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS416 Gal-RNQ1

    Plasmid

    #18684

    Depositor

    Susan Lindquist

    Article

    Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

    Insert

    Rnq1 (RNQ1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

13. C453-E02: attB4-CAGp>-attB5

    Plasmid

    #162972

    Purpose

    Gateway attB4/attB5 entry clone for mammalian expression using the CAG promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    chicken beta-actin/CMV enhancer promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. C453-E24: attB4-UbCp>-attB5

    Plasmid

    #162980

    Purpose

    Gateway attB4/attB5 entry clone for mammalian expression using the human Ubiquitin C promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    human Ubiquitin C promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C512-E23: attB5r-mRuby3-nostop-attB1r

    Plasmid

    #162945

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal mRuby3 fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    monomeric Ruby3 fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. C512-E22: attB5r-mClover3-nostop-attB1r

    Plasmid

    #162944

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal mClover3 fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    monomeric Clover3 fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. C238-E02: attB2r-BGHpolyA-attB3

    Plasmid

    #162916

    Purpose

    Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Bovine growth hormone polyadenylation sequence
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His6_BcLOV4_C292A_mCherry_BamUK

    Plasmid

    #119761

    Purpose

    Encodes mCherry-tagged BcLOV4 "dark" mutant with C292A mutation for expression in bacterial cells

    Depositor

    Brian Chow

    Article

    Glantz et al Methods Enzymol. 2019;622:249-270. doi:

    Insert

    BcLOV4-C292A_mCherry
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Mutated cysteine at position 292 to alanine

    Promoter

    T7

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMTB-Multibow-mfR

    Plasmid

    #60991

    Purpose

    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKate2.

    Depositor

    Sean Megason

    Article

    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

    Insert

    mKate2
    

    Use

    Zebrafish

    Tags

    membrane tag

    Expression

    Mutation

    Promoter

    Bactin2

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pFUS_B8

    Plasmid

    #31025

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge