We narrowed to 8,785 results for: Ott

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pCS2-MCRS1-GFP-IRES-mCherry

Plasmid

#231231

Purpose

MCRS1 stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MCRS1 (MCRS1 Human)

Expression

Mammalian

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-ChloC

Plasmid

#179841

Purpose

donor plasmid for cardiac-specific CCND2-T2A-Chloc expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

chloride-conducting ChR (ChloC)

Expression

Mammalian

Promoter

Troponin T promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-iChloC

Plasmid

#179842

Purpose

donor plasmid for cardiac-specific CCND2-T2A-iChloc expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

improved chloride-conducting ChR (iChloC)

Expression

Mammalian

Promoter

Troponin T promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-luciferase

Plasmid

#179844

Purpose

donor plasmid for cardiac-specific CCND2-T2A-luciferase expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR, Luciferase, and TALEN ; Donor plasmid

Tags

luciferase

Expression

Mammalian

Promoter

Troponin T promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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LMPd Amt PHGDH

Plasmid

#209409

Purpose

Retroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffold

Depositor

Russell Jones

Article

Ma et al Immunity. 2019 Nov 19;51(5):856-870.e5.

Insert

Phgdh shRNA (Phgdh Mouse)

Use

Retroviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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SITS-MmCav1-APEX2

Plasmid

#170526

Purpose

Vector expression of MmCav1-APEX2 in Leishmania cell free system. Parton lab clone IYW

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Caveolin1 (Cav1 Mouse)

Use

Leishmania cell free expression clone

Tags

APEX2 and Lifeact

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-PARK7H126A

Plasmid

#115180

Purpose

Gateway cloning of PARK7H126A into any destination vector

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7H126A (PARK7 Human)

Use

Gateway cloning into appropriate destination vect…

Mutation

p.H126A

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-PARK7E163K

Plasmid

#115181

Purpose

Gateway cloning of PARK7E163K into any destination vector

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7E163K (PARK7 Human)

Use

Gateway cloning into appropriate destination vect…

Mutation

p.E163K

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2

Plasmid

#115192

Purpose

Lentiviral transduction and expression of PDHA2 into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PDHA2WT (PDHA2 Human)

Use

Lentiviral

Promoter

EF1alpha

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S291A

Plasmid

#115196

Purpose

Lentiviral transduction and expression of PDHA2S291A into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PDHA2S291A (PDHA2 Human)

Use

Lentiviral

Mutation

p.S291A

Promoter

EF1alpha

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S291A/S293A

Plasmid

#115198

Purpose

Lentiviral transduction and expression of PDHA2S291A/S293A into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PDHA2S291A/S293A (PDHA2 Human)

Use

Lentiviral

Mutation

p.S291A/S293A

Promoter

EF1alpha

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-PARK7V51G

Plasmid

#115178

Purpose

Gateway cloning of PARK7V51G into any destination vector

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7V51G (PARK7 Human)

Use

Gateway cloning into appropriate destination vect…

Mutation

p.V51G

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-PARK7C53A

Plasmid

#115179

Purpose

Gateway cloning of PARK7C53G into any destination vector

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7C53A (PARK7 Human)

Use

Gateway cloning into appropriate destination vect…

Mutation

p.C53A

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPHAGE-EF1α-Luc-C20orf24-3’UTR-VAR

Plasmid

#128507

Purpose

Lentiviral constitutive expression of Firefly luciferase under control of 3'UTR of human C20orf24 with variant from COX deficiency patient.

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA-NONO-1

Plasmid

#127654

Purpose

Knock-out of human NONO (guide only)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

Insert

NONO sgRNA (NONO Human)

Expression

Mammalian

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARMA2sh-delta458-500

Plasmid

#97074

Purpose

Expresses mutant CARD14/CARMA2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

Insert

CARD14 isoform 3 (CARD14 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 458-500

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Gfi1-exon5 (F3)-GFP

Plasmid

#91884

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F2)-GFP

Plasmid

#91877

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F1)-GFP

Plasmid

#91883

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
Lenti-LucA

Plasmid

#174047

Purpose

Expresses mALG8 (ITYTWTRL) antigen as a fusion to luciferase and Cre recombinase

Depositor

Tyler Jacks

Article

Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.

Insert

LucA

Use

Cre/Lox, Lentiviral, and Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

ALG8 alanine 506 to threonine

Promoter

Human Ubiquitin C

Availability

Academic Institutions and Nonprofits only

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