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YFP Rab4DN

Plasmid

#99019

Purpose

Localization and visualization of the dominant negative form of Rab4

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

Rab4 DN (Rab4 Synthetic, Fly)

Tags

YFP

Expression

Mammalian

Mutation

S22N Dominant negative

Promoter

CMV

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCAT024

Plasmid

#171636

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-BFP
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC6-GPT1-3xFLAG

Plasmid

#163448

Purpose

lentiviral overexpression vector for human GPT1

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

GPT1 (GPT Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-neo largeTcDNA delta434-444

Plasmid

#8582

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Mutation

deletion in part of the bipartite p53 binding dom…

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
GCN2-KD1-pGEX4T-1

Plasmid

#21876

Depositor

David Ron

Article

Harding et al Mol Cell. 2000 Nov . 6(5):1099-108.

Insert

GCN2 (Eif2ak4 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

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ELF5_pet28a

Plasmid

#131659

Purpose

Bacterial expression of ETS gene ELF5

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF5 (ELF5 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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3XFlag DVL2 (∂PY)

Plasmid

#24808

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

DVL2 (DVL2 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of the PY motif: PPPY

Available Since

June 22, 2010

Availability

Industry, Academic Institutions, and Nonprofits
wt HA-dynamin 1 pUHD10-3

Plasmid

#34688

Depositor

Sandra Schmid

Article

Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

Insert

dynamin 1 (DNM1L Human)

Tags

HA (variant)

Expression

Mammalian

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pT2ADW-lox-mCherry-hyBRET-ERK-NLS

Plasmid

#170290

Purpose

For generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

lox-mCherry-lox-hyBRET-ERK-NLS

Expression

Mammalian

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBS U6 WASp RNAi

Plasmid

#11775

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WASp RNAi (WAS Human)

Use

RNAi

Expression

Mammalian

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pLenti-hSyn-Gαq*-BERKY1

Plasmid

#158430

Purpose

BRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSyn

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-YFP-GRK2(RH)

Use

Lentiviral

Expression

Mammalian

Promoter

hSynapsin

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMX-Ascl1-puro

Plasmid

#198227

Purpose

Retroviral expression of Ascl1

Depositor

Li Qian

Article

Wang et al Cell Stem Cell. 2022 Oct 6;29(10):1491-1

Insert

Ascl1 (Ascl1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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mEmerald-MCM6-N-22

Plasmid

#54172

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM6

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pENTR-QK1

Plasmid

#16183

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Quaking (QKI Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pBK-CMV-mUBP43

Plasmid

#12459

Depositor

Dong-Er Zhang

Article

Liu et al Mol Cell Biol. 1999 Apr . 19(4):3029-38.

Insert

UBP43 (Usp18 Mouse)

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
KA1875_pGL4-UBCmin-luc2/Sox2_SRR2

Plasmid

#124198

Purpose

Luciferase reporter driven by the Sox2 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Sox2 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pWB-Flag-IKKe

Plasmid

#44115

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

IKKe (IKBKE Human)

Use

Retroviral

Tags

Flag

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pGL3E-hIL-17prom(-232)-Luc

Plasmid

#25628

Depositor

Sarah Gaffen

Article

Liu et al J Biol Chem. 2004 Dec 10. 279(50):52762-

Insert

human IL-17 promoter (IL17A Human)

Use

Luciferase

Available Since

June 25, 2010

Availability

Academic Institutions and Nonprofits only
C1-FKBP(F36V):mCherry-Akt2(K14A)

Plasmid

#72906

Purpose

Recruitable Akt2(K14A) mutant

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

RAC-beta serine/threonine-protein kinase (AKT2 Human)

Tags

FKBP(F36V), mCherry

Expression

Mammalian

Mutation

K14A mutation

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-tdTomato

Plasmid

#153424

Purpose

Retroviral (MSCV) expression of tdTomato fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

tdTomato

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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