Addgene

We narrowed to 13,669 results for: Egf

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pCMV5-Flag-Ago2-FDM3

Plasmid

#72210

Purpose

Mammalian expression of aa 227-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-226

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM1

Plasmid

#72208

Purpose

Mammalian expression of aa 1-372 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 373-860

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM2

Plasmid

#72209

Purpose

Mammalian expression of aa 1-517 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 518-860

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM4

Plasmid

#72211

Purpose

Mammalian expression of aa 373-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-372

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM5

Plasmid

#72212

Purpose

Mammalian expression of aa 518-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-517

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pHR-ZIP(FF)

Plasmid

#27135

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Tyrosines in ITAMS 1-3 mutated to phenylalanines …

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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KDR gRNA (BRDN0001146819)

Plasmid

#77755

Purpose

3rd generation lentiviral gRNA plasmid targeting human KDR

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KDR (KDR Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KDR gRNA (BRDN0001146648)

Plasmid

#77756

Purpose

3rd generation lentiviral gRNA plasmid targeting human KDR

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KDR (KDR Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KDR gRNA (BRDN0001144938)

Plasmid

#77757

Purpose

3rd generation lentiviral gRNA plasmid targeting human KDR

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KDR (KDR Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FLT1 gRNA (BRDN0001145564)

Plasmid

#75557

Purpose

3rd generation lentiviral gRNA plasmid targeting human FLT1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FLT1 (FLT1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FLT1 gRNA (BRDN0001147168)

Plasmid

#75559

Purpose

3rd generation lentiviral gRNA plasmid targeting human FLT1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FLT1 (FLT1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FLT1 gRNA (BRDN0001145335)

Plasmid

#75556

Purpose

3rd generation lentiviral gRNA plasmid targeting human FLT1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FLT1 (FLT1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS1162

Plasmid

#29077

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple94 (GPR88 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Aug. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-TACC3KDP∆682-688-shTACC3

Plasmid

#69116

Purpose

Dual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
shTACC3

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of amino acids 682-688 (region containin…

Promoter

CMV

Available since

Oct. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pJet-CMV-IARS1[W435C]-GFP

Plasmid

#212323

Purpose

EGFP-tagged IARS1 Trp435Cys

Depositor

Sabine Fuchs

Article

Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

Insert

IARS (IARS1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Trp435cys

Promoter

CMV

Available since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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macroH2A2-GFP (pc2191)

Plasmid

#223709

Purpose

Expression of macroH2A2 tagged N-terminal to GFP

Depositor

Cristina Cardoso

Article

Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

Insert

macroH2A2-GFP (MACROH2A2, H2AFY2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgFlt4#2/Cre

Plasmid

#193216

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Flt4 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgFlt4#2 (Flt4 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgFlt4#1/Cre

Plasmid

#193215

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Flt4 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgFlt4#1 (Flt4 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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px458-AMBRA1 #3

Plasmid

#172607

Purpose

Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

gRNA against AMBRA1 (AMBRA1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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RG6-BAP1_E11

Plasmid

#154025

Purpose

Bichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 11

Depositor

Samuel Peña-Llopis

Article

Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

Insert

BAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)

Use

Splicing reporter

Tags

DsRed1, FLAG, SV40 NLS, and eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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RG6-BAP1_E7

Plasmid

#154023

Purpose

Bichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 7

Depositor

Samuel Peña-Llopis

Article

Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

Insert

BAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)

Use

Splicing reporter

Tags

DsRed1, FLAG, SV40 NLS, and eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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FLT4 gRNA (BRDN0001147158)

Plasmid

#75629

Purpose

3rd generation lentiviral gRNA plasmid targeting human FLT4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FLT4 (FLT4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FLT4 gRNA (BRDN0001147213)

Plasmid

#75630

Purpose

3rd generation lentiviral gRNA plasmid targeting human FLT4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FLT4 (FLT4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FLT4 gRNA (BRDN0001149037)

Plasmid

#75632

Purpose

3rd generation lentiviral gRNA plasmid targeting human FLT4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FLT4 (FLT4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FLT1 gRNA (BRDN0001146782)

Plasmid

#75558

Purpose

3rd generation lentiviral gRNA plasmid targeting human FLT1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FLT1 (FLT1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

Plasmid

#162877

Purpose

Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Insert consists of AA1-170

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.GFP

Plasmid

#57818

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

EFS and hU6

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-α-cateninA-

Plasmid

#229698

Purpose

Transient expression of GFP-alpha-cateninA- in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L785A, I792A, V796A

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-FLT4

Plasmid

#116745

Purpose

Lentiviral expression of FLT4

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FLT4 (FLT4 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-α-cateninΔβH

Plasmid

#229705

Purpose

Transient expression of GFP-alpha-catenin-delta-beta-H in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge