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pHR_Pax6-RE_tBFP_PGK_mCitrine

Plasmid

#188388

Purpose

Lentiviral vector - tBFP reporter for Pax6-based SNIPRs with a constitutive mCitrine

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

Pax6-RE_tBFP_PGK_mCitrine

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL2-pCMV7.1

Plasmid

#155020

Purpose

N-terminal 3xFLAG-tagged CUL2 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 2 (CUL2 Human)

Tags

3xFLAG

Expression

Bacterial

Promoter

pCMV7.1

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGGF_RT_FAST_RFP

Plasmid

#136979

Purpose

Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

Depositor

Klaus Theres

Article

Rossmann et al Plant J. 2019 Oct 30. doi: 10.1111/tpj.1

Insert

seed coat marker RFP

Expression

Bacterial

Mutation

BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRARα.hRXRα

Plasmid

#135411

Purpose

Human Retinoic Acid Receptor-alpha and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Inserts

Human retinoic acid receptor-alpha
Human retinoid X receptor-alpha

Expression

Mammalian

Promoter

CMV and SV40

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBN267

Plasmid

#86723

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Prgef-1::FLP D5

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJG/BETA MHC promoter

Plasmid

#53963

Purpose

transgene expression driven by BETA MHC promoter

Depositor

Jeffrey Robbins

Article

Rindt et al J Biol Chem. 1993 Mar 5;268(7):5332-8.

Type

Empty backbone

Expression

Mammalian

Promoter

beta MHC

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-G9-5FLAG-kanMX6

Plasmid

#49181

Purpose

genomic G9-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

G9-5FLAG

Expression

Yeast

Available Since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pJRT.Agro.CLCrVA.010

Plasmid

#31810

Depositor

Niki Robertson

Article

Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi:

Insert

JRTCLCrVA.010

Use

Agrobacterium binary

Available Since

Nov. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-3HA

Plasmid

#39283

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Mutation

-1163 to +6 of nmt1 locus

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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myc-NLRC5 K234A

Plasmid

#37523

Depositor

Thomas Kufer

Article

Neerincx et al J Immunol. 2012 May 15;188(10):4940-50.

Insert

NLRC5 (NLRC5 Human)

Tags

myc

Expression

Mammalian

Mutation

K234A

Promoter

CMV

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
WZLneo-PPARγ2

Plasmid

#34566

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

PPARγ2 (Pparg Mouse)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

5'LTR

Available Since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only
TALEN1297

Plasmid

#32279

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting hey2 (hey2 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pBRIT Pax7 TAP

Plasmid

#17528

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

TAP

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pBS-M71-IRES-CreFNF

Plasmid

#15509

Depositor

Peter Mombaerts

Article

Li et al Nature. 2004 Mar 25. 428(6981):393-9.

Insert

M71 targeting vector with IRES CreFNF

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
3535 pET15b Bid p22

Plasmid

#8784

Depositor

Stanley Korsmeyer

Article

Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

Insert

Bid p22 (Bid Mouse)

Tags

His

Expression

Bacterial

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pFA6a-mScarletI-KanMX6

Plasmid

#136690

Purpose

Tagging genes in Cterm with mScarletI in fission yeast

Depositor

Julien Berro

Insert

mScarletI

Tags

mScarletI

Expression

Yeast

Promoter

None

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 tdTomato LIC cloning vector (6F)

Plasmid

#86963

Purpose

Empty LIC vector; adds a tdTomato gene to the C-terminus of your protein of interest.

Depositor

Chris Jeans

Type

Empty backbone

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mTagBFP2-Blasticidin

Plasmid

#167207

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mTagBFP2

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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LoxP1-His-H2B-Cherry-2A (SO290)

Plasmid

#99616

Purpose

To clone gene of interest downstream of LoxP1-His-H2B-Cherry-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

6xHis-H2B-Cherry

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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LoxP2-H2B-EGFP-V5-2A (SO107)

Plasmid

#99617

Purpose

To clone gene of interest downstream of LoxP2-H2B-EGFP-V5-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

H2B-EGFP-V5

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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ORF57 Pr pGL4.16

Plasmid

#120378

Purpose

Firefly luciferase reporter with KSHV ORF57 early gene promoter

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF57 Promoter

Use

Luciferase

Promoter

ORF57 Promoter

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(1)_T2ALexA

Plasmid

#125221

Purpose

Artificial phase 1 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

LexA

Use

Other

Tags

T2A

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(1)_T2AGAL4

Plasmid

#125212

Purpose

Artificial phase 1 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL4

Use

Other

Tags

T2A

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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B108-GFP-IRES-AP

Plasmid

#73988

Purpose

B108 enhancer of Blimp1 gene drives EGFP expression.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

B108 enhancer

Expression

Mammalian

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGKO1

Plasmid

#63611

Purpose

a binary Agrobacterium vector carrying the HSVtk gene as a negative selection marker against ectopic transformants.

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Binary fungal vector

Promoter

Lac Promoter

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pcrRNA.ind

Plasmid

#61284

Purpose

Arabinose-inducible repeat-only E. coli CRISPR array

Depositor

Chase Beisel

Article

Luo et al Nucleic Acids Res. 2014 Oct 17. pii: gku

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pSWEET-bgaB

Plasmid

#210272

Purpose

Xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.

Depositor

Eric Brown

Article

Bhavsar et al Appl Environ Microbiol. 2001 Jan;67(1):4

Insert

bgaB

Expression

Bacterial

Promoter

PxylA

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-FLPo-3xP3-RFP

Plasmid

#127561

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

FLPo

Use

CRISPR

Expression

Insect

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-11

Plasmid

#119990

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet-i

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBN312

Plasmid

#86734

Purpose

GFP with Frt sites for FLP-mediated knock-out of gene function in C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

GFP_Frt_KO

Expression

Worm

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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