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pRK172-α-syn-TEV-GFP

Plasmid

#166671

Purpose

Bacterial expression of alpha synuclein coupled to GFP via a TEV site for uptake studies

Depositor

Iris Lindberg

Article

Jarvela et al PLoS One. 2021 Jan 26;16(1):e0241161. do

Insert

mouse synuclein (from V. Lee) (Snca Mouse)

Use

Tags

GFP

Expression

Bacterial

Mutation

added a TEV cleavage site sequence between synucl…

Promoter

CMV

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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YFP-S100A10

Plasmid

#107200

Purpose

Expresses YFP-human S100A10 fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Zobiack et al J Cell Sci. 2002 Jan 1;115(Pt 1):91-8.

Insert

S100A10 (S100A10 Human)

Use

Tags

Yellow Fluorescent Protein

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCALNL-Rheb(S16H)

Plasmid

#194204

Purpose

Cre/LoxP-dependent expression of a constitutively active form of Rheb (S16H). Expression of Cre excises a STOP codon before Rheb(S16H), facilitating expression of a constitutively active form of Rheb.

Depositor

Angelique Bordey

Article

Lin et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

Ras Homolog Enriched Protein in Brain (S16H) (RHEB Human)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

S16H

Promoter

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK

Plasmid

#25033

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

Rck (DDX6 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7

Plasmid

#62276

Purpose

mammalian expression of human Alpha7 (CHRNA7)

Depositor

Sherry Leonard, Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRNA7 (CHRNA7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pT7-EGFP-C1-HsEDC3

Plasmid

#25032

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

EDC3 (EDC3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pCDNA3-V5-PTPN14-wild type

Plasmid

#61003

Purpose

Expresses wild type human PTPN14 in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 (PTPN14 Human)

Use

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
pIND(V5)EcoDam

Plasmid

#59201

Purpose

Mammalian DamID vector used to fuse gene of interest to V5-tagged E.coli Dam. Can also express Dam alone as negative control.

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Mutation

Promoter

Heat Shock Minimal Promoter

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2s-L-Venus

Plasmid

#19966

Depositor

Jonathan Javitch

Article

Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

Insert

D2 short variant-monomeric Venus (DRD2 Human, Aequorea Victoria)

Use

Tags

Flag and monomeric Venus

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2009

Availability

Academic Institutions and Nonprofits only

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canxTM-mEmerald (cytosolic)

Plasmid

#186962

Purpose

Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

CANX (CANX Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Only express calnexin (482-end) and add ER signal…

Promoter

CMV Promoter

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLgw EcoDam-V5-RFC1

Plasmid

#59209

Purpose

Mammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloning

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Lentiviral; Damid

Tags

Dam and V5

Expression

Mammalian

Mutation

Promoter

Heat Shock Minimal Promoter

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7A-mCherry

Plasmid

#62635

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mCherry fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

Expression

Mammalian

Mutation

mCherry inserted into M3-M4 loop

Promoter

CMV

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pDK637

Plasmid

#134203

Purpose

bacterial expression of human CENP-N 1-240

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-N 1-240-His (CENPN Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD-Myc

Plasmid

#201166

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

MESV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pWzyE-distrepII

Plasmid

#196667

Purpose

Expression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Use

Tags

TEV-cleavable di-strep tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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integrin beta6 pcDNA1 neo

Plasmid

#13580

Depositor

Dean Sheppard

Article

Weinacker et al J Biol Chem. 1994 Mar 4. 269(9):6940-8.

Insert

integrin beta 6 (ITGB6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7-YFP

Plasmid

#62630

Purpose

mammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

hAlpha7 (CHRNA7 Human)

Use

Tags

Expression

Mammalian

Mutation

YFP inserted in M3-M4 loop

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2s-L-V1

Plasmid

#19967

Depositor

Jonathan Javitch

Article

Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

Insert

D2 short variant-N terminal Venus (DRD2 Human, Aequorea Victoria)

Use

Tags

Flag and N terminal Venus

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2009

Availability

Academic Institutions and Nonprofits only
pClneoMyc human MST2

Plasmid

#37022

Depositor

Yutaka Hata

Article

Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

Insert

MST2 (STK3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-flex-TVA

Plasmid

#69618

Purpose

Express recepter TVA for envA with AAV

Depositor

Naoshige Uchida

Article

Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

Insert

Avian sarcoma and leukemia virus receptor 950 (CD320 quail, Synthetic)

Use

AAV and Cre/Lox

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pClneoMyc human CIN85

Plasmid

#47935

Purpose

expresses Myc tagged human CIN85 in mammalian cells

Depositor

Yutaka Hata

Article

Kawata et al J Biochem. 2006 May;139(5):931-9.

Insert

CIN85 (SH3KBP1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

contains amino acids 2-665 of human CIN85; D114G

Promoter

CMV

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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mEmerald(luminal)-canxTM

Plasmid

#186961

Purpose

Expresses mEmerald connected with the N-terminus of Calnexin transmembrane domain.

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

CANX (CANX Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Only express calnexin (482-end) with ER signal pe…

Promoter

CMV Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-V5-PTPN14-PPxY

Plasmid

#61006

Purpose

Expresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 PPxY mutation (PTPN14 Human)

Use

Tags

V5 tag

Expression

Mammalian

Mutation

PPxY mutation

Promoter

CMV

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
pINDgw RFA-V5-EcoDam

Plasmid

#59204

Purpose

Mammalian DamID vector for fusing gene of interest with Dam-V5 using Gateway cloning

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Mutation

Promoter

Heat Shock Minimal Promoter

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsDCP1a_G

Plasmid

#146400

Purpose

Mammalian Expression of HsDcp1a

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDcp1a (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T237C and G1716T compared to…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7A-mGFP

Plasmid

#62633

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mEGFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

mEGFP inserted in M3-M4 loop

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc Separase Non-Cleavable

Plasmid

#59826

Purpose

Allows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Non-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pST_AfrLEA3m (pBS0911)

Plasmid

#185285

Purpose

For the mammalian expression of the brine shrimp protein AfrLEA3m. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfrLEA3m

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2s-L-V2

Plasmid

#19968

Depositor

Jonathan Javitch

Article

Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

Insert

D2 short variant-C terminal Venus (DRD2 Human, Aequorea Victoria)

Use

Tags

C terminal Venus and Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2009

Availability

Academic Institutions and Nonprofits only
pKM296

Plasmid

#134180

Purpose

Mammalian expression of CENP-C and KNL2 CDK SA

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

mcherry-CENP-C 510-end-KNL2 CDK SA (CENPC Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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