We narrowed to 5,149 results for: pcDNA3.1+

1. pcDNA3.1 FLIPglu-3.2mDelta13V

   Plasmid

   #18011

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   FLIPglu F16A S112A Delta13
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   F16A, S112A in binding protein

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rat NCX1.7 (pcDNA3.1+)

   Plasmid

   #75192

   Purpose

   Mammalian expression of SLC8A1

   Depositor

   Jonathan Lytton

   Article

   Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

   Insert

   SLC8A1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-HA-mSTING (V154M)

   Plasmid

   #214159

   Purpose

   Transient expression of gain-of-function STING mutant

   Depositor

   Nan Yan

   Article

   Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

   Insert

   STING (V154M) (Sting1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N153S

   Promoter

   CMV

   Available since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pY012 (pcDNA3.1-hCMtCpf1)

   Plasmid

   #69984

   Purpose

   Expresses humanized CMtCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hCMtCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. mcherry Gamma 9 ---pcDNA3.1

   Plasmid

   #64203

   Purpose

   G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

   Depositor

   Narasimhan Gautam

   Article

   Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

   Insert

   G protein Gamma 9 (GNGT2 Human)
   

   Use

   Tags

   6xHis and mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

6. Mouse NCKX3 (pcDNA3.1+)

   Plasmid

   #75204

   Purpose

   Mammalian expression of SLC24A3

   Depositor

   Jonathan Lytton

   Article

   Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

   Insert

   SLC24A3 (Slc24a3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)_Granulin 2+linker3

   Plasmid

   #176920

   Purpose

   express Granulin 2 with linker 3 in mammalian cells

   Depositor

   Thomas Kukar

   Article

   Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

   Insert

   Granulin 2+linker3 (GRN Human)
   

   Use

   Tags

   Twin-Strep and FLAG tags encoded after Signal Pep…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 myc-PITPalpha

   Plasmid

   #58277

   Purpose

   mammalian expression of human PITP alpha

   Depositor

   Shamshad Cockcroft

   Insert

   PITP alpha (PITPNA Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1(+)-ExRai-AKAR(T/A)

   Plasmid

   #118406

   Purpose

   Negative-control mutant for ExRai-AKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR(T/A)
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pF153 pcDNA3.1(+)SOD1G37R

    Plasmid

    #26399

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G37R

    Promoter

    Available since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1-Renilla-ccdB

    Plasmid

    #87068

    Purpose

    Mammalian Gateway-compatible expression vector backbone with an N-terminal Renilla luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1/Hygro(-)-B2-TS3

    Plasmid

    #104353

    Purpose

    Expresses human beta 2 integrin tension sensor

    Depositor

    Pontus Nordenfelt, Timothy Springer

    Article

    Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

    Insert

    Human integrin b2 with tension sensor (ITGB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AA1-733 ITGB2 present

    Promoter

    CMV

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 MYC dLZ

    Plasmid

    #176048

    Purpose

    Expresses Myc Mutant (delta leucine zipper) in Mammalian cells

    Depositor

    Linda Penn

    Article

    Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

    Insert

    Myc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted amino acids 414-433

    Promoter

    CMV

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pY017 (pcDNA3.1-hPcCpf1)

    Plasmid

    #69989

    Purpose

    Expresses humanized PcCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPcCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-aDTX-lumitoxin

    Plasmid

    #51686

    Purpose

    Expression vector for aDTX-lumitoxin

    Depositor

    Edward Boyden

    Article

    Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

    Insert

    aDTX-lumitoxin
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+)-Rab10-FLAG

    Plasmid

    #135559

    Purpose

    Express mouse Rab10 in mammalian cells, FLAG at N terminal

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Rab10 (Rab10 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F

    Plasmid

    #159132

    Purpose

    Expresses BACH1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    BACH1 (BACH1 Human)
    

    Use

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Y11F

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)-TTF-1-HDD

    Plasmid

    #52063

    Purpose

    It encodes a mutant of TTF-1 in which the homeodomain is deleted

    Depositor

    David Mu

    Article

    Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

    Insert

    Thyroid Transcription Factor 1 (NKX2-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Homeodomain deleted

    Promoter

    Available since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1-CHRFAM7A-mGFP

    Plasmid

    #62633

    Purpose

    mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mEGFP fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    CHRFAM7A (CHRFAM7A Human)
    

    Use

    Tags

    mEGFP inserted in M3-M4 loop

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pY005 (pcDNA3.1-hLb3Cpf1)

    Plasmid

    #69977

    Purpose

    Expresses humanized Lb3Cpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLb3Cpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA3.1+_LN-HA-AGO2-WT

    Plasmid

    #92009

    Purpose

    Expresses Lambda-N-HA-AGO2 (WT)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Tags

    Lambda-N-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1(+)-FluoSTEP-AKAR

    Plasmid

    #181844

    Purpose

    Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    FluoSTEP-AKAR
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-alpha4 wt (Hygro)

    Plasmid

    #80017

    Purpose

    Mammalian expression of human integrin alpha4, Hygro selection

    Depositor

    Chinten James Lim

    Article

    Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

    Insert

    integrin alpha4 (ITGA4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pY117 (pcDNA3.1-huMb3Cpf1)

    Plasmid

    #92293

    Purpose

    Expression of humanized Mb3Cpf1

    Depositor

    Feng Zhang

    Insert

    huMb3Cpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-MS2-HA-HsNeuroguidin_AA

    Plasmid

    #148237

    Purpose

    Mammalian Expression of HsNeuroguidin

    Depositor

    Elisa Izaurralde

    Insert

    HsNeuroguidin (NGDN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-Hyg-BFP

    Plasmid

    #214363

    Purpose

    Expression of the Blue Fluorescent Protein

    Depositor

    Bertrand Collet

    Article

    Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

    Insert

    BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pY120 (pcDNA3.1-huFbCpf1)

    Plasmid

    #92296

    Purpose

    Expression of humanized FbCpf1

    Depositor

    Feng Zhang

    Insert

    huFbCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Rat NCX1.3 (pcDNA3.1+)

    Plasmid

    #75191

    Purpose

    Mammalian expression of SLC8A1

    Depositor

    Jonathan Lytton

    Article

    Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

    Insert

    SLC8A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1_mGFP-FGFR3-mScarlet

    Plasmid

    #192509

    Purpose

    Expression of N-terminally mGFP and C-terminally mScarlet labelled wildtype FGFR3 (isoform IIIc)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc-mScarlet
    

    Use

    Tags

    mGFP and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1_mGFP-FGFR3-G380R

    Plasmid

    #191755

    Purpose

    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-G380R mutation (associated with ACH; target mutation: c.1138G>A; silent mutation added on purpose: c.1131C>T)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    c.1138G>A (G380R) + c.1131C>T (silent mutat…

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge