We narrowed to 11,973 results for: SOM
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Plasmid#210224PurposeIntegrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.DepositorInsertmNeongreen Lac repressor fusion protein
UseTrypanosoma brucei expressionPromoterPol II readthrough transcription after integrationAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX307 ASC
Plasmid#163977PurposeMammalian expression vector encoding ASC (for example, to assess ASC-speck formation or inflammasome signalling assays)DepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-iRFP-EEA1
Plasmid#174453PurposeExpress iRFP with early endosome-targeting sequence in mammalian cellsDepositorInsertiRFP with early endosome-targeting sequence
Tagsearly endosomal antigen 1 (EEA1)ExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Q58L wgMDH
Plasmid#204246PurposeBacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationQ58LPromoterT5Available SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-POC5
Plasmid#136786PurposeMammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tagDepositorInsertSNAP-POC5 (POC5 Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-STIL
Plasmid#136795PurposeMammalian expression of the centrosomal protein STIL N-terminally fused to SNAP-tagDepositorInsertSNAP-STIL (STIL Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-WT
Plasmid#177614PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshiftingDepositorInsertSARS-CoV-2 FSE
UseLuciferaseExpressionMammalianAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MC177VSG221RNAi
Plasmid#59730PurposeFor use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.DepositorInsertVSG221 RNAi fragment
UseRNAiPromoteropposing T7 promotersAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus
Plasmid#182344PurposesgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.DepositorInsertSpacer 4
ExpressionWormAvailable SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBB HA cIAP1 H588A
Plasmid#38233DepositorAvailable SinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124K wgMDH
Plasmid#204258PurposeBacterial expression of R124K wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR124KAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124Q wgMDH
Plasmid#204259PurposeBacterial expression of R124Q wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR124QAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130E wgMDH
Plasmid#204267PurposeBacterial expression of R130E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR130EAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pEBTetBl-CLIP-STIL
Plasmid#136844PurposeMammalian expression of the centrosomal protein STIL N-terminally fused to CLIP-tagDepositorInsertCLIP-STIL (STIL Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-ChR2-Kv-P2A-H2b-mRuby2
Plasmid#89570PurposeChannelrhodopsin localized to neuronal soma with simultaneous nuclear labelingDepositorInsertsChannelrhodopsin
Histone 2B
UseAAVTagsmRuby2ExpressionMammalianMutationH134RPromotersynapsinAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
PLK1-cjun-FRET-PACT biosensor
Plasmid#106907Purposecentrosome-localized PLK1 FRET-activity biosensor (through PACT domain with 10aa linker)DepositorInsertPLK1-cjun-FRET-PACT biosensor with linker (PLK1 Human)
TagsPACT domainExpressionMammalianPromoterCMVAvailable SinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Centrin3
Plasmid#136806PurposeMammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tagDepositorInsertSNAP-Centrin3
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET17b-yebST
Plasmid#175804PurposeComplementation of yebST knockout in E. coli K-12DepositorInsertyebST
TagsNoneExpressionBacterialMutationsilent mutations to introduce restriction sitesPromoterT7Available SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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WT (REP10)
Plasmid#21368DepositorInsertautosomal dominant polycystic kidney disease type I (PKD1 Human)
ExpressionMammalianAvailable SinceJan. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196M wgMDH
Plasmid#204273PurposeBacterial expression of R196M wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR196MAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 D193E wgMDH
Plasmid#204269PurposeBacterial expression of D193E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationD193EAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAAV Syn ChR E90R-T159C 2A tDimer
Plasmid#52495PurposeHistorical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)DepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVExpressionMammalianMutationE90R, T159CPromoterhuman synapsin-1 and ribosomal skip sequenceAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-FOP
Plasmid#136796PurposeMammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tagDepositorInsertSNAP-FGFR
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry2-sgC9cen
Plasmid#198326PurposeExpresses a guide RNA against a repetitive locus found in the pericentromere of human chromosome 9, with mCherry2 reporterDepositorInsertChr9-CEN guide RNA
ExpressionMammalianPromoterhU6Available SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-FOP
Plasmid#136797PurposeMammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tagDepositorInsertSNAP-FGFR
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-Centrin3
Plasmid#136807PurposeMammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tagDepositorInsertSNAP-Centrin3
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
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TPC2-mCherry
Plasmid#240468PurposeRed-tagged lysosomal channelDepositorInsertTPC2N2 (TPCN2 Human)
TagsmCherryExpressionMammalianMutationMutated aspartate 276 to lysine.PromoterCMVAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-IvfChr-citrine-KV2.1
Plasmid#221615PurposeA recombinant AAV2 plasmid encoding vfChrimson-citrine with trafficking enhancement and soma targeting with KV2.1 motif.DepositorInsertvfChrimson-citrine with membrane trafficking enhancement and soma targeting
UseAAVMutationvfChrimson-citrine with membrane trafficking enha…PromoterHuman SynapsinAvailable SinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM-601[insert(+1) TA-rich]
Plasmid#221196Purpose601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601DepositorInsert601[insert(+1)TA-rich]
UseUnspecifiedMutation601 has an additional mutation added 22 bp from d…Available SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G121A wgMDH
Plasmid#204252PurposeBacterial expression of G121A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationG121AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196E wgMDH
Plasmid#204272PurposeBacterial expression of R196E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR196EAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123A wgMDH
Plasmid#204254PurposeBacterial expression of P123A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationP123AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130Q wgMDH
Plasmid#204268PurposeBacterial expression of R130Q wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR130QAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127F wgMDH
Plasmid#204264PurposeBacterial expression of G127F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationG127FAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only