We narrowed to 728 results for: ELP
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Plasmid#124618PurposeExpresses SpyDock to bind SpyTag non-covalently for affinity purificationDepositorInsertSpyDock
TagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-SAM
Plasmid#102559PurposePiggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.DepositorInsertsMS2-P65-HSF1_T2A_Hygro
dCAS9(D10A, N863A)-VP64_T2A_Blast
UseCRISPR; Piggybac transposonExpressionMammalianMutationD10A and N863A in Cas9 and N55K in MS2PromoterCAG and EF1AAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKS016 - pCAGGS-3XFLAG-mKate2-Pds5a-bpA
Plasmid#156439PurposeFor transient expression of mouse PDS5A tagged with mKate2DepositorAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA
Plasmid#156440PurposeFor transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2DepositorAvailable SinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-MFA-NFAPoptim
Plasmid#221090PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ERDepositorTypeEmpty backboneExpressionYeastAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
WT USP7 catalytic
Plasmid#227201PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationaa 207-560PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA
Plasmid#156445PurposeFor transient expression of mouse RAD21 (SCC1) tagged with mKate2DepositorAvailable SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA
Plasmid#156436PurposeFor transient expression of human PDS5B tagged with mKate2DepositorAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA
Plasmid#156441PurposeFor transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2DepositorAvailable SinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS2-TVA-eGFP-N2cG
Plasmid#175439PurposeCre-on (DIO) helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoerDepositorInsertTVA-P2A-eGFP-P2A-N2cG
UseAAV and Cre/LoxAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-MFA-NFAPoptim
Plasmid#221095PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ERDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRedCmOcr
Plasmid#176226PurposeA helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.DepositorInsertLambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyExpressionBacterialPromoterPrhaB and PLAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW17 - pAdapter
Plasmid#174664PurposeConstitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system.DepositorInsertMS2 coat protein
TagsλCIExpressionBacterialMutationV30I, A81G, positions 68-80 deleted (VATQTVGGVELP…PromoterSynthetic Constitutive PromoterAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
p35u4
Plasmid#174786PurposeConstitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid systemDepositorInsertMS2 coat protein
TagsλCIExpressionBacterialMutationV30I, A81G, positions 68-80 deleted (VATQTVGGVELP…PromoterSynthetic Constitutive PromoterAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330-EN1082_Rad21_STOP
Plasmid#156450PurposeFor transient expression of Cas9 and sgRNA targeting mouse RAD21 stop codonDepositorAvailable SinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHDR-eGFP (pXK-1647)
Plasmid#208824PurposeThe HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDRDepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS5-TVA-eGFP-N2cG
Plasmid#175440PurposeCre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoerDepositorInsertTVA-P2A-eGFP-P2A-N2cG
UseAAV and Cre/LoxAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Reckleen_3_multieditdropout
Plasmid#233458PurposeLevel 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniaeDepositorInsertLevel 2 RECKLEEN plasmid, Ptac lambda Red operon, Ptet SpG cas9, J23117 acrIIA4
UseCRISPRAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCiGSΔΨ
Plasmid#108312PurposeFoamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.DepositorInsertgag
ExpressionMammalianMutationpCiGSΔΨ is a newer version of the pCiGS Gag expre…PromoterCMVAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKS037 - pCAGGS-3XFLAG-Smc3-mKate2-bpA
Plasmid#156447PurposeFor transient expression of mouse SMC3 tagged with mKate2DepositorAvailable SinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only